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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and utilized Alt-R CRISPR-Cas9 crRNA/trRNA/Hi-Fi Cas9 ribonucleotide-protein complex (IDT) and Neon electroporation (ThermoFisher) to deliver the complex to the iPSC ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were visualized using Ponceau stain as well as Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Protein detection was performed by incubation with a 6x-His Tag mouse monoclonal antibody (clone: HIS.H8; Thermo Fisher Scientific), followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N ...
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bioRxiv - Immunology 2023Quote: ... Proteins containing a His-tag were purified by affinity chromatography using HisPur Ni-NTA resin (Thermo scientific, Cat#88222). Proteins were then eluted with 250 mM imidazole in 50 mM Tris-HCl and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... and the 3C protease was removed using Dynabeads magnetic beads designed for His-tagged protein isolation (Thermo Fisher, 10103D). The supernatant from the magnetic bead pulldown was then concentrated using Amicon 10 kDa centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content of each pre-cleared sample was determined using Pierce bicinchoninic acid assay protein assay (Thermo Fisher Scientific). To measure luciferase activity ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were then measured by bicinchoninic acid (BCA) assay (Micro BCA™ Protein Assay Kit, Cat # 23235; Thermo Scientific). The resulting supernatant yielded low volumes (30-40 µl of supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... the protein concentration of each fraction was determined using a bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific). Samples were stored at −80 °C until further use.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Total protein concentrations of the samples were determined by the Pierce™ bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, USA), and samples were aliquoted and stored at −80°C until further use ...
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bioRxiv - Microbiology 2022Quote: ... trichloroacetic acid was performed as described previously 37 and protein concentration was determined by BCA protein assay kit (Thermo scientific). Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentrations of the lysates were then quantified using a bicinchoninic acid protein kit (Thermo Fisher Scientific, Rockford, IL). Aliquots containing 12 µg of total cellular lysates for each sample were denatured at 100 °C for 5 min and separated using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biochemistry 2021Quote: ... The remaining soluble protein fraction was collected and quantified for protein concentration via bicinchoninic acid (BCA) assay (Pierce Thermo Fisher). The soluble protein concentration was then used as an indication of lysis efficiency/cell mass to determine the amount of 8 M urea (dissolved in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of EL fractions and TL were determined using the Bicinchoninic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Neuroscience 2022Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Molecular Biology 2022Quote: The protein concentrations of renal samples were determined with a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). Protein samples (50 μg ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/ml bFGF recombinant human protein (Thermo Fisher Scientific, USA). From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Human recombinant epidermal growth factor (hEGF) protein was purchased from Life Technologies and dissolved in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 ng/mL Human TGF-β 1 recombinant protein (Cat# PHG9204, Gibco) and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2020Quote: ... The protein content was determined using the bicinchoninic acid (BCA) assay (Thermo Fisher Scientific). To prepare inactivated concentrated viruses ...
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bioRxiv - Microbiology 2020Quote: ... The protein content was determined using the bicinchoninic acid (BCA) assay (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations of brain lysates were determined with the bicinchoninic acid (Thermo Fisher, 23225) assay and normalized to 2 mg/ml ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... The protein concentration of the lysate was quantified using bicinchoninic acid (BCA) assay (ThermoFisher). 100 μg of protein from each sample were transferred and final concentration of 20 mM ammonium bicarbonate ...
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bioRxiv - Neuroscience 2019Quote: ... Pierce Bicinchoninic Acid (BCA) protein assay kit was obtained from Thermo Scientific (Rockford, IL), and ProSignal Blotting Film were purchased from Genesee Scientific (El Cajon ...
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bioRxiv - Physiology 2022Quote: ... and the amount of protein was measured using the bicinchoninic-acid assay (BCA; ThermoFisher) and a freshly prepared bovine serum-albumin (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the BCA (bicinchoninic acid) assay (Thermo Fisher Scientific, USA).
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were quantified using the bicinchoninic Acid (BCA) assay (Pierce, Thermo Fisher 23225). Protein samples were normalized and prepared in 1X LDS Sample Buffer (NuPAGE LDS Sample Buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Opti-MEM and Pierce BCA (bicinchoninic acid) protein assay kit were from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was then determined using a bicinchoninic acid (BCA) assay kit (ThermoFisher Scientific). The lysate was diluted to 8 mg/mL with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, 23225). Supernatants were re-suspended in NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... The protein concentrations were determined by the bicinchoninic acid (BCA) method (Thermo Scientific, USA). The immunoblotting assays were conducted as described (63 ...
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bioRxiv - Biochemistry 2020Quote: ... EVs protein content was quantified by bicinchoninic acid (BCA) assay (Thermo Scientific Pierce™)27 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were evaluated using Brachidonic acid assay kit (Thermo Fisher Scientific, Cat# 23227). Equal loads of proteins were separated on Mini-PROTEAN TGX gel (Bio-rad ...
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bioRxiv - Cancer Biology 2019Quote: ... and protein concentration was determined by Pierce BCA (bicinchoninic acid) assay (Thermo Fisher Scientific). 8-30μg of whole-cell extract was separated on precast 4 to 12% bis tris protein gel (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... through the use of Pierce Bicinchoninic Acid Assay (BCA) Protein Assay Kit (Thermo Scientific) method ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilized proteins in the supernatant were quantified using Bicinchoninic acid (BCA) assay (Life Technologies), 650μg-1mg of the lysate was added to Protein A-Sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Sample protein content was quantified in triplicates by BCA (Bicinchoninic Acid) assay kit (ThermoFisher) at 590nm using a microplate reader (Synergy HT ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined using the bicinchoninic acid (BCA) method (Thermo Scientific, cat # 23227). Gel samples were prepared by mixing 30μg of protein with LDS sample buffer (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with the bicinchoninic acid (BCA) reagent (Thermo Scientific, Waltham, MA). 20-30 μg of protein extract was loaded onto 4 to 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...