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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for En 1948 4 Who Dioxin Like Pcb "Starter Kit" since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The isolated total RNA was quantified using a Qubit 4 fluorometer and the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, USA), and the RNA quality was assessed using a Bioanalyzer 2100 System (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: 500ng of PCR product per sample was pooled and PCR product pool was quantified on Qubit 4 Fluoremeter using Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific) and finally diluted to a 10nM solution for sequencing on Illumina HiSeq2500 (FaDu ...
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bioRxiv - Neuroscience 2020Quote: ... Extracts were centrifuged at 15.000g for 10 min at 4°C and supernatants were collected and quantified with Pierce™ BCA Protein Assay Kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured neurons were transfected with plasmids (1.5 μg of plasmid DNA per well) at 4 - 5 d in vitro (DIV) using a commercial calcium phosphate transfection kit (Thermo Fisher Scientific) as previously described21,24.
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bioRxiv - Plant Biology 2021Quote: DNA was extracted from 4 week old M82 plants using GeneJET Plant Genomic DNA Purification Mini Kit (Thermo Scientific, Waltham, MA, USA). DNA-Seq libraries were prepared based on BrAD-seq (Townsley et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
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bioRxiv - Cell Biology 2021Quote: Indicated cells were labelled with 20 μM 5-ethynyl-2′-deoxyuridine (EdU) for 4 h and processed following the manufacturer’s protocol (Click-iT® EdU Alexa Fluor® 488 Imaging Kit, Thermo Fisher). Prior to imaging cells were then stained with 5ng/ml Hoecsht 3342 for 15 minutes ...
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bioRxiv - Physiology 2021Quote: Total RNA from around 20 whole flies of both sexes at 4-7 days old was extracted using a PureLink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific, USA). Reverse transcription (RT ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from each sample was converted to cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368813). Primer pairs used for this study are listed below.
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Genomics 2021Quote: ... The concentrations of the libraries were measured on a Qubit 4 fluorometer using the ds DNA HS Assay Kit (Thermo Fisher Scientific). The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: Indicated cells were labelled with 20 µM 5-ethynyl-2’-deoxyuridine (EdU) for 4 h and processed following the manufacturer’s protocol (Click-iT® EdU Alexa Fluor® 488 Imaging Kit, Thermo Fisher). Prior to imaging cells were then stained with 5ng/ml Hoecsht 3342 for 15 minutes ...
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was cleared by centrifugation at 20,000 x g for 30 min at 4 °C and purified by using GE Healthcare His GraviTrap™ Kit (Cat. No. 45-002-016, Fisher Scientific). After elution ...
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bioRxiv - Cancer Biology 2023Quote: ... spun at 13,000 rpm for 30 minutes at 4°C and supernatant protein concentration determined using the BCA assay kit (#23225; Thermo Fisher Scientific). 30-50 μg aliquots of lysate proteins were resolved on sodium dodecyl sulfate-7.5% polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Microbiology 2023Quote: ... and quantified using the Invitrogen Qubit 4 Fluorometer with the High Sensitivity Qubit kit following the manufacturer’s guidelines (Invitrogen, Carlsbad, CA, USA). Each of the 60 purified PCR products per strain were normalized ...
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bioRxiv - Microbiology 2023Quote: ... Isolated gDNA was quantified using the Invitrogen Quibt 4 Fluorometer with the Broad Range Qubit kit following the manufacturer’s guidelines (Invitrogen, Carlsbad, CA, USA). The integrity of gDNA was determined by running 5 µl of each gDNA sample on a 1 % agarose gel.
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... 4 μg of total RNA per sample was used for ribosomal RNA depletion using Dynabeads mRNA Purification Kit (Thermo Fisher Scientific, USA) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and cells were spun onto glass slides using a Cytospin 4 Cytocentrifuge (Thermo Scientific), dried for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were migrated on precast Novex 4–20% or 4–12% polyacrylamide gels (Thermo Fisher Scientific), then transferred to Novex nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... only 4 μL of 1 mM FM 4-64 (Cat. number T13320, Thermo Fisher, Waltham, MA) was injected ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native-PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific). To induce expression of the hGRAD and TRIM21 systems ...
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bioRxiv - Biochemistry 2024Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Biophysics 2020Quote: ... 4 mM glutamine GlutaMAX™ (Gibco) and 1x non-essential amino acids (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 mM L-glutamine (Life Technologies), 1 mM sodium pyruvate (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μL of Lipofectin (Life Technologies) and 1 μg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... Isopropanol (Thermo Fisher Scientific - A416-4), LDL (Alfa Aesar - J65039) ...
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bioRxiv - Neuroscience 2021Quote: ... and 4% FBS (10042682; Fisher Scientific) (Placzek et al. ...
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bioRxiv - Immunology 2021Quote: ... and 4 units of RNaseOUT (ThermoFisher). Following sorting ...
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bioRxiv - Bioengineering 2019Quote: ... 4 mM L-glutamine (Life Technologies), 10 mM HEPES (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... 100ng/ml IL-4 (Gibco, USA) for 48h ...
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bioRxiv - Neuroscience 2019Quote: ... and 4-12% NuPAGE (Thermo Scientific) Bis-Tris gels for ECC proteins ...
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bioRxiv - Cell Biology 2020Quote: ... 4 nM Calyculin A (PHZ1044; Invitrogen) or 1 mM 4-hydroxyacetophenone (278564 ...
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bioRxiv - Developmental Biology 2021Quote: ... on the Qubit 4 fluorometer (Invitrogen). RNA quality was assessed using the RNA 6000 Nano kit total RNA assay (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/ml IL-4 (Gibco), 10 ng/ml IL-10 (Gibco) ...