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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... in a QuantStudio 7 qPCR instrument (Applied Biosystems). Cp values for each target gene (primers HCCS_qF1 ...
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bioRxiv - Cell Biology 2024Quote: ... on the QuantStudio 7 Pro system (ThermoFisher Scientific). Relative gene expression was calculated by the ΔΔ Ct method ...
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bioRxiv - Cancer Biology 2024Quote: ... the CellEvent Caspase3/7 Green Detection Reagent (Invitrogen) was added to each well at a final concentration of 2μM ...
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bioRxiv - Neuroscience 2024Quote: ... on a ViiA 7 system (Thermo Fisher Scientific). ATP5O and TUBB3 were employed as calibrator genes ...
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bioRxiv - Cell Biology 2024Quote: ... Sept-7 1/800 (Thermo scientific, PA5-54755), GAPDH 1/5000 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 μg CRISPR plasmid and 7 μg non-linearized HDR template using Lipofectamine 3000 according to the instructions (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... and the cell pellet was resuspended in annexin buffer with Annexin V-FITC and 7-AAD according to the manufacturer’s specifications (FITC Annexin V 7-AAD-Thermo Fisher®). Flow cytometry was performed using Attune flow cytometer (Thermo Fisher®) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with FACS buffer and incubated with the live-dead marker 7-aminoactinomycin D (7-AAD; Thermo Fisher Scientific) for 10 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis induction was determined by activation of Caspase 3 and 7 using the CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen).H460 cells were plated at 5 × 103 cells/well in black 96 well plates with clear bottoms (Costar ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were treated for 1h at 37 °C with DNase I (1U per mg of RNA; Ambion), extracted again with Trizol LS and precipitated.
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bioRxiv - Genomics 2020Quote: ... Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D, Invitrogen, ThermoFisher Scientific). Final DNA was purified with MinElute (#28004 ...
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bioRxiv - Genomics 2020Quote: ... Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D, Invitrogen, ThermoFisher Scientific). Final DNA was purified with MinElute (#28004 ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 cells were infected at an MOI of 0.01 for 1h in serum-free DMEM (Thermo Fisher) at 33°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... cells were centrifuged at 38,720g for 1h using a Sorvall RC 3C Plus centrifuge (Thermo Scientific, Waltham, MA). The supernatant that contained the soluble fusion protein was filtered over Millex-GP syringe-driven 0.22-µm PES membranes (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: 5µm-pore transwell inserts (Costar 3421) have been coated for 1h at 37°C with gelatin (Gibco S006100) and 10µg/ml bovine fibronectin protein (Thermo Fisher 33010018) ...
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bioRxiv - Plant Biology 2024Quote: ... before being incubated for 1h in solution containing 10 μM Alexa 488-azide (Invitrogen A10266, Thermo Fisher Scientific) and 100 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... before being incubated for 1h in solution containing 10 μM Alexa 488-azide (Invitrogen A10266, Thermo Fisher Scientific) and 100 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... endogenous peroxidases were first quenched by incubating the material for 1h in a peroxidase suppressor solution (Thermo Scientific). After six baths of 5min in 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were collected and pre-cleared for 1h at room temperature with Dynabeads protein G magnetics beads (Invitrogen) blocked with yeast tRNA (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 1h with primary antibodies followed by 30 minutes with secondary antibodies (Alexa Fluor, Thermo Fisher Scientific) in 1% BSA ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly isolated MNCs were washed and re-suspended in PBS and stained for 10 min at 37°C with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) at a concentration of 5 µM (Thermo Fisher Scientific, Waltham, MA, USA). H2DCFDA is a non- fluorescent dye which is cleaved inside cells to 2’,7’-dichlorofluorescein (H2DCF) ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Physiology 2022Quote: ... The mitochondria membrane potential was also assessed in cells stained with 50 nM tetramethyl rhodamine ethyl ester (TMRE, ThermoFisher, T669) and subsequently collected in HBSS containing 5 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... y229Gt (Tg(tnks1bp1:EGFP)) transgenic larvae were exposed to 250 nM tetramethylrhodamine ethyl ester perchlorate (TMRE, Thermo Fisher, Cat# T669) diluted in EM for 25 minutes while wrapped in foil ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of ion-pairing agent (0.1% o-toluidine blue, Carolina Biological) and 3 µL of ethyl acetate (ACS grade, Fisher Scientific). The mixture was vortexed and centrifuged briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher, C10423) in 96 well plates ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reactions for the data corresponding to Figure 7 and Figure 7 – figure supplement 1 were carried out in 384-well plates on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... and undigested chromatin were adjusted to 810μl with MCN-digestion buffer with 0.005M EGTA and cross-linked by adding 135 μl of buffered 7 % formaldehyde solution (7 % CH2O, 140 mM Na2HPO4 pH 8.67) using 16 % CH2O methanol-free ampules (Thermo Scientific Cat #28906), to a final concentration of 1 % ...
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bioRxiv - Cancer Biology 2021Quote: ... QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... in ViiA 7 Real-time PCR Instrument (Applied Biosystems). The Standard deviation calculation and quantification were done from three independent experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Claudin-7 (Invitrogen, 34-9100, Carlsbad, CA, USA), anti-VDR (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... Human hepatocellular carcinoma cells (Huh-7; Thermo Fisher Scientific) and African green monkey kidney cells (Vero E6 ...
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bioRxiv - Developmental Biology 2021Quote: ... on a ViiA 7 real-time PCR System (ThermoFisher). The list and sequences of the primers used are presented in Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... containing 7% gamma-irradiated fetal bovine serum (FBS, Gibco). SS2-1 stored in 50% glycerol was kept in JAAS-IVM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... for 7 min and washed in PBS (Thermo Fisher Scientific ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... for 7 minutes to a nitrocellulose membrane (Invitrogen, IB301002). Membranes were subsequently blocked with a 5% milk solution in tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Plant Biology 2019Quote: ... in ViiA 7 Real-Time PCR System (Applied Biosystems). Relative expression was calculated in enzyme or 17-β-estradiol treated leaves with respect to mock/control (buffer or 0.1% DMSO ...