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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 4 4 Bromophenylsulfonyl morpholine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... ∼4×10^4 hESCs were plated onto a single well of a Vitronectin-coated 12-well dish (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Immunology 2024Quote: ... The cells were cultured for 2 weeks and then washed and fixed in 4% paraformaldehyde (4% PFA; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads; Invitrogen) were washed on a magnetic stand (ThermoFisher ...
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bioRxiv - Genetics 2021Quote: ... and cytocentrifuged on clean slides (using a Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific, at 900 rpm for 4 min). Slides were then immersed in liquid nitrogen for 5 min ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... for 20 min at 4 °C and were then centrifuged at 444g for 4 min and dissociated to single cells using StemPro Accutase (Invitrogen). Cells were subsequently fixed using 4% paraformaldehyde (PFA ...
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Six hundred μg of the supernatant were incubated for 4 hours under rotation at 4°C with 20 μl of protein A Dynabeads (Invitrogen) containing anti-UNR antibody or rabbit IgG as control in a final volume of 600 μl ...
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bioRxiv - Plant Biology 2021Quote: ... GUS activity was determined by measuring the fluorescence (excitation wavelength 365 nm and emission wavelength 460 nm) of produced 4-Methylumbelliferon (4-MU) in a 96well polystyrene microwell plate (Nunc F96 black flat bottom, ThermoFisher) by the Tecan Infinite 200 PRO microplate reader ...
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bioRxiv - Immunology 2020Quote: ... 4-incubation with 4 μg/mL secondary goat anti rabbit conjugated with AlexaFluor(AF)488 (goat IgG H+L, Invitrogen) in microscopy buffer for 1 h at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Stained lenses were washed three times in 4°C Medium 199 and then 200 μL 4°C PBS (Gibco, MA) was added to each well prior to measuring DHR fluorescence intensity at Ex/Em of 507/529 nm and Hoescht 33342 at Ex/Em of 360/460 nm with a microplate reader (Spectramax M3 ...
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bioRxiv - Neuroscience 2022Quote: Brains were post-fixed overnight at 4’C in 4% PFA then cryoprotected in 30% (w/v) sucrose (Fisher Scientific) solution in PBS for at least 48 hours prior to processing ...
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bioRxiv - Immunology 2022Quote: ... and 125) with TSt-4 (B cells) or TSt-4/DLL1 stromal cells (T cells) in RPMI (Thermo Fisher Scientific) supplemented with 10% BSA (093001 ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Neuroscience 2022Quote: ... and the samples were first postfixed overnight in 4 % PFA (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 d for decalcification ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then denatured by incubating samples at 95°C for 5 min in 4 x Laemmli buffer and dithiothreitol (DTT) before loading onto 4 to 12% NuPAGE gradient precast gels (ThermoFisher). Gels were run for 10 min at 110 V ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated overnight in 30% sucrose in PBS buffer after overnighted fixation at 4°C in 4% paraformaldehyde in PBS buffer (Invitrogen) until the intestinal tissues completely sink to the tube bottom ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed three times for 10 min in 1X PBS and incubated for 4 h at 4°C with the fluorescently tagged secondary antibodies (Invitrogen; AlexaFluor 647 goat anti-mouse and AlexaFluor 488 donkey anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed with 4% paraformaldehyde and stored at 4°C until blocked with 10% donkey serum in Phosphate-Buffered Saline (PBS) (Invitrogen) and 0.25% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Samples were normalized by BCA quantifications and then loaded in 4 12% or 4-20% Novex Tris-glycine gels (Invitrogen) and run at 165V for ∼1 h in Tris-glycine buffer (25 mM Tris and 192 mM glycine ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Spleen tissues were fixed in 4% formaldehyde overnight at 4°C and then cut with a microtome (Micron KS34 ThermoFisher) to obtain 30 μm tissue sections86 that were then stained for 10 min with 0.3 µM DAPI in DPBS (Thermo Scientific™ #62247) ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Neuroscience 2024Quote: ... 3% dilution, 4 µl,), Fluoro-ruby (FR, case BC, (10% solution, mixture of 10 kDA and 3 kDA, 4 µl; Invitrogen), and Biotinylated Dextran Amine (BDA ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Molecular Biology 2024Quote: The redox state of Trx3 was measured by covalent modification with the thiol-reactive probe 4-acetamido-4’maleimidyldystilbene-2,2’-disulfonic acid (AMS; Molecular Probes) as described previously 49 ...
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bioRxiv - Physiology 2024Quote: ... Exendin-4-BSA-AF647 (Ex-4-BSA-AF647) was obtained by conjugation of Ex-4-BSA with Alexa Fluor 647 NHS ester (#A20006, Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... were run over Novex Wedge well 4–12% Tris-Glycine and NuPAGE 4–12% Bis-Tris and polyacrylamide gels (both from Invitrogen), respectively ...
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bioRxiv - Biophysics 2024Quote: ... and blotted for 4-6 s at 4°C and 100% humidity using the Vitrobot Mark IV system (Thermofisher Scientific), before plunging immediately into liquid ethane for vitrification ...