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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 4 2 Methylimidazol 1 ylmethyl phenylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... S2P6 was serially diluted 1:4 starting at 80 μg/ml in infection media (DMEM supplemented with 2% FBS and 20mM HEPES (Gibco, 15630-080)) and incubated with replication-competent VSV-SARS-CoV-2 (23 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2024Quote: ... Cell concentrations were assessed by acquiring 30 μL of resuspended cells diluted 1:5 in PBS-4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) without any prior washing steps ...
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bioRxiv - Cancer Biology 2024Quote: ... on a rotating wheel for 2 hours at 4°C and 1 hour more incubation with 1.5mg Dynabeads protein G (Thermo Fisher Scientific, 10004A). IP fractions were washed four times with lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were detected by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA).
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bioRxiv - Molecular Biology 2020Quote: ... and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific) at 1:2,000 dilution at RT for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were counterstained in 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D1306) for 10 minutes at room-temperature and mounted in ProLong Gold Antifade (Thermo fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were labelled using 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Invitrogen) and coverslipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: Cultured DRG neurons were loaded with 4 μM Fura-2 AM (Life Technologies) in culture medium at 37°C for at least 60 minutes before use ...
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bioRxiv - Cell Biology 2021Quote: LX-2 and TWNT-4 human HSCs were lysed in TRIzol (Life Technologies) and total RNA was purified following the supplied protocol ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Life Technologies) at a dilution of 1:5000 in 1 X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counterstained using 4’ ,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 mL of 2 mM disuccinimidyl glutarate (DSG,Life Technologies, #20593, Carlsbad, CA) prepared in 1X PBS was added per 1 × 107 cells to the conical tube and the solution was mixed thoroughly by pipetting to remove clumps ...
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bioRxiv - Microbiology 2020Quote: ... Samples were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei and finally washed with PBS.
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bioRxiv - Biophysics 2020Quote: ... 2 mM MgCl2) and 4 μM of the phosphobinding protein (PBP) (Life Technologies) to detect the inorganic phosphate (Pi ...
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bioRxiv - Cancer Biology 2021Quote: ... The nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probes) at 1 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were staining with DAPI (4′,6-diamidino-2-phnylindole) from Molecular Probes. Confocal images were acquired using a Leica Sp8 confocal microscope and processed using Imaris image analysis software (version 9.3.1).
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bioRxiv - Cancer Biology 2022Quote: ... sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH; 22589, Thermo Fisher Scientific), 3-(Acryloyloxy)propyltrimethoxysilane (L16400 ...
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bioRxiv - Immunology 2022Quote: ... Nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI) dihydrochloride (Life Technologies), and lung sections were mounted on glass microscopy slides using fluorescence mounting medium (Dako) ...
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bioRxiv - Cancer Biology 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI, 0.1 μg/mL; #D1306, Invitrogen) and mounted with Prolong Gold antifade medium (#P10144 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with 4′,6-diamidino-2-pheny-lindoldihydrochloride (DAPI, Thermo Fisher Scientific #D3571) diluted in DPBS for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole dihydrochloride; Molecular Probes). Pictures were taken using a TCS SP5 Inverted confocal (Leica ...
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bioRxiv - Genetics 2021Quote: ... Tissue slides were also stained with DAPI (4′,6-diamidino-2-phenylindole, Invitrogen) to visualize the total number of nucleated myocardial cells within each section ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were incubated in 4’,6-diamidino-2-phenylindole (DAPI, Acros Organics–Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... cells were counterstained using 4’,6-Diamidino-2-Phenylindole (DAPI – Life Technologies, D1306) diluted in PBS before being mounted on microscope slides with ProLong Gold Antifade Mountant (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... P2X7a 451L or P2X7a 451P were loaded with 2 μM Fluo-4 (Invitrogen) for 20 min at 4°C and 10 min at 37°C ...
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bioRxiv - Genomics 2021Quote: ... the DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) diluted 1:10,000 with PBST ...
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bioRxiv - Cell Biology 2021Quote: ... Fluoromount-G mounting medium with 4′,6-diamidino-2-phenylindole (Invitrogen, Waltham, MA) was used to mount the samples.
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bioRxiv - Developmental Biology 2020Quote: ... XEN cells were passaged every 2–4 days using TrypLE Express (Thermo Fisher) up to passage 20.
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bioRxiv - Microbiology 2023Quote: ... Cells were mounted with ProLong and 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen) and imaged using a Zeiss Imager M2 Plus wide field fluorescence microscope ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated with 4’,6-diamidino-2-phenylindole (DAPI Thermo Fisher Scientific) and AlexaFluor 555 anti-mouse (Thermo Fisher Scientific ...