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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 1 4 Bromo 3 methylphenyl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-mouse Alexa-Fluor 488, 1:500, Thermofisher Scientific, 4 hrs at RT; Th: goat anti-rabbit Alexa-Flour 488, 1:500, Thermofisher Scientific, 4 hrs at RT). Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs).
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bioRxiv - Cell Biology 2020Quote: ... plasmids (1 μg of guide RNAs +/- 3 μg donor) were diluted in 1 ml OptiMem (Gibco) and 20 ul PEI (1 mg/ml) ...
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bioRxiv - Bioengineering 2021Quote: ... Caspase 3/7 assay solution was mixed 1:1 with αMEM without phenol red (ThermoFisher Scientific) (caspase 3/7 lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... 1/10 volume of 3 M sodium acetate and 1 μL glycogen 10 mg/mL (Thermofisher). Pellet obtained by centrifugation 15000 x g ...
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bioRxiv - Cell Biology 2024Quote: ... mIMCD-3 cells were cultured in 1:1 DMEM high-glucose pyruvate (41966052, GIBCO, Life Technologies): F-12 Nutrient Mixture (21765037 ...
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bioRxiv - Cell Biology 2024Quote: ... mIMCD-3 cells were cultured in 1:1 DMEM high-glucose pyruvate (41966052, GIBCO, Life Technologies): F-12 Nutrient Mixture (21765037 ...
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bioRxiv - Immunology 2024Quote: ... cells were diluted 1:3 in PBS with 2 mM EDTA and 1:1000 DAPI (ThermoFisher). Cells were counted using Forward Scatter (FSC ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 70 µm nylon colander (Falcon) with 3-4 ml of Ca2+- and Mg2+-free HBSS medium containing 10 % fetal bovine serum (FBS, Gibco), 1 % MEM-vit (Gibco ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Molecular Biology 2021Quote: ... COCS were incubated for 3 days at 4°C with secondary antibodies Alexa594-conjugated goat anti-rabbit IgG (Life Technologies) or Alexa647-conjugated goat anti-rat IgG (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then centrifuged at 300 g for 3 min and fixed with 4 mM 3,3’– dithiobis(sulfosuccinimidyl propionate) (DTSSP, Life Technologies) in PBS at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: Group 3 MB cells (3×105) were seeded overnight and subsequently serum-starved for 4 hours prior to transient transfection using Lipofectamine 2000 (Invitrogen) for 6 hours in serum-free media with miR-212-3p mimic (25 nM and 50 nM ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM MEK inhibitor PD0325901 (Wako, Tokyo, Japan), 3 μM GSK3 inhibitor CHIR99021 (Wako, Tokyo, Japan) and Penicillin-Streptomycin (Invitrogen). Blastocyst outgrowths were disaggregated and passaged onto the new wells plated with feeder cells in the same medium condition ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... DTT concentration in the buffer was reduced by dialysis against TEDG buffer with DTT 0.1 mM at 4 ºC overnight using a 3 KDa pore size dialysis cassette (ThermoFisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... reinhardtii cells were grown at room temperature for 3-4 days in the light in 20 mL of TAP media (ThermoFisher). The algae were collected by centrifugation at 10,000 x g for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 1.3163 g/cm3) and ultracentrifuged for 3 hours at 180,000 × g (average) at 4°C (TH-641 rotor, Thermo Fisher, thinwall polypropylene tube with 13.2 ml capacity ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Biophysics 2022Quote: ... were washed three times in sterile PBS and opsonized overnight at 4°C in 3 mg/mL mouse IgG (Invitrogen). To remove excess antibody ...
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bioRxiv - Microbiology 2020Quote: ... or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16, Thermo Fisher Scientific) in a modified SC medium ...
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bioRxiv - Plant Biology 2021Quote: ... TRV:NRC2/3/4 plasmid (Wu et al., 2016; Wu et al., 2017) by Phusion High-Fidelity DNA Polymerase (Thermo Fisher) using primers listed in SI Appendix ...
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed thrice in PBS and individually incubated with MCDB131 growth medium supplemented with 4 µM Fluo 3-AM (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and 143B cybrid cells were passaged every 3-4 days at 80% confluence and trypsinised using 0.25% trypsin-EDTA (Gibco #25200056). Similarly ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Immunology 2021Quote: ... A force of -4 was used to blot excess liquid for 3 s using a Vitrobot Mark IV (Thermo Fisher) followed by plunge-freezing with liquid ethane ...
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bioRxiv - Immunology 2020Quote: ... The excess liquid was blotted for 3 seconds with a force of −4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...