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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... HEPES (10 nM, 15630080, Gibco), hydrocortisone (1 μg∕ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... containing 10% FBS (Thermo Fisher), 100 units/mL penicillin (Corning) ...
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TAK1 operates at the primary cilium in non-canonical TGFB/BMP signaling to control heart developmentbioRxiv - Developmental Biology 2024Quote: ... supplemented with 10% FBS (Gibco) and 1% Penicillin-Streptomycin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FBS (Gibco). HT29 CDH3 knockout cell line was made with a viral vector that expressed Cas9 and a pCAD guide RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FBS (Gibco). All cells were cultured at 37°C under 5% CO2 and passaged before reaching confluency up to 25 passages ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% FBS (Gibco) and 100 U/ml penicillin/streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 10% FBS (Thermo Fisher Scientific), 2 mM Glutamax (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 10% tryptose phosphate broth (Gibco), 100 U/mL of penicillin–streptomycin ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10 % FBS (Gibco), 1 % penicillin/streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... The secondary antibodies used for detection were horseradish peroxidase (HRP)–conjugated goat anti-mouse and goat anti-rabbit IgG (1:5,000; Thermo Scientific). The blots were incubated with Luminata Western HRP substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... was used as secondary antibody at 1:10,000 dilution and the detection was achieved with the ECL Femto reagent (Thermo Fisher). The V5 tag was detected with an anti-V5 antibody coupled with HRP (SantaCruz ...
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bioRxiv - Cell Biology 2020Quote: ... primary antibody at a dilution of 1:100 and secondary detection was performed using donkey anti-rabbit Alexa 488 antibodies (Invitrogen) at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... primary antibody at a dilution of 1:100 and secondary detection was performed using donkey anti-mouse Alexa 647 antibodies (Invitrogen). pRB staining was performed following EdU staining by first permeabilizing the cells and then blocking in donkey serum for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using SYBR green Supermix (OZYME) in an ABI7500 Prism SDS Real-Time PCR Detection System (Applied Biosystems). The mRNA content was normalized to β-actin mRNA and quantified using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: All of the PCR reactions were performed using an ABI Prism 7900HT Sequence Detection system (Thermo Fisher Scientific, Waltham, Massachusetts). PCR was performed using the Power SYBR™ Green PCR Master Mix (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Cycling was performed in duplicate using a Step One Plus™ Real Time detection system (Applied Biosystems, Foster City, CA). Gene-specific amplification was normalized to GAPDH as an internal reference gene and gene expression was normalized relative to uninfected MDSC controls using the formula 2-ΔΔCt.
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems), using System SYBR-green fluorescence (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... and detection carried out with fluorescent-labeled secondary antibodies (goat anti-rat, 1:800, Alexa Fluor 488, A-101650, Invitrogen). For the detection of mCherry endogenous fluorescent protein animals were anesthetized with Avertin ...
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bioRxiv - Cancer Biology 2021Quote: Feature detection and peak alignment from .Raw files were performed using Compound Discoverer 3.2 (Thermo Fisher Scientific, Waltham, MA, USA). Files were also converted to .mgf format using MSConvert software (ProteoWizard ...
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bioRxiv - Neuroscience 2020Quote: Glutamate concentration in microdialysis samples was quantified using isocratic high-performance liquid chromatography (HPLC) with electrochemical detection (Thermo Fisher Scientific). O-pthalaldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Both membranes were subsequently incubated for 1 hour at RT with the Clean-Blot IP Detection Reagent (HRP, Thermo Scientific). After washing the membrane three times with 1x TBST ...
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bioRxiv - Neuroscience 2021Quote: ... was used for the PCR reaction was used according to the manufacturer’s protocol in a QuantStudio3 Detection System (Applied Biosystems). Each sample was run in duplicate and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Cell Biology 2020Quote: To detect proliferation/cell cycle entry we utilized the EdU/click-it detection platform to visualize cells that contained newly synthesized DNA (Cat nb. C10337 or C10340, Invitrogen, Thermofisher). For experiments performed immediately post FACS ...
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bioRxiv - Cell Biology 2021Quote: ... MS-HRM-PCR analysis was done in 7500 FAST Real Time ABI Prism Sequence Detection System (Applied Biosystems, Boston, EUA) using temperature ramp and acquisition of fluorescence in accordance with the manufacturers’ recommendation ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with PBS and detection was performed using an appropriate fluorescent secondary antibody (Alexa Fluor 488, 568, or 647, Invitrogen). Samples were mounted in DAPI containing antifade mounting medium ...
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bioRxiv - Bioengineering 2022Quote: ... conjugated to horseradish peroxidase (HRP) were used for detection of proteins using ECL Western Blot Substrate Reagent (Thermo Fisher Scientific). Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 more times in 1x PBS before DNA detection with 1 µg/ml Hoechst 33342 (Invitrogen, H3570) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were visualized using the Pierce ECL 2 Western Blotting Substrate detection method (0.5 pg, lower limit sensitivity; Thermo Scientific). The images were obtained and analyzed with ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time RT-PCR measurements were performed using the Step One Plus Real-Time Detection System (Thermo Scientific, Waltham, USA). Reactions were performed in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... or mouse antibody to Myc (1:2000, Sta. Cruz) and Clean-Blot IP detection reagent (HRP) (1:5000, Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... All QPCR reactions were performed in duplicate on the 7500 ABI Prism Sequence detection System (Applied Biosystems, Foster City, CA). The cycling conditions included a denaturation step of 95°C for 10 mins followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Microbiology 2022Quote: ... then washed three times with PBS and incubated with secondary antibodies (Table 4) and phalloidin (F-actin detection) (1:100) (Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in an ABI StepOnePlus instrument with the SYBR green detection system (Invitrogen). PCR thermal profiles were 40 cycles with 10 s at 95 °C and 30 s at 60 °C each cycle ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Microbiology 2020Quote: ... The thermal cycle and fluorescence detection were performed with the QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... The arrays were washed again with distilled water (G biosciences) and incubated with a detection buffer with Cy5-streptavidin (ThermoFisher) at 1:1000 for 20 minutes at RT protected from light ...
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: All quantitative PCR (qPCR) experiments were conducted using an ABI 7900HT sequence detection system (Perkin-Elmer Applied Biosystems, Warrington, UK). Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunofluorescence detection in confocal and SIM microscopy were Alexa Fluor Rabbit-anti-Mouse 568 (Invitrogen, A11004) and Donkey-anti-Mouse 647 (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated overnight in a shaker protected from light at 4°C with respective detection reagents (DyLight 488-conjugated streptavidin at 1:400 dilution, Invitrogen #21832 ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Real Time qRT-PCR was performed using SYBR-green based detection on a Quant Studio 3 real time PCR system using PowerUP Syber green MasterMix (ThermoFisher). Primers for GAPDH and beta-actin were used as endogenous controls to which RNA levels of target genes were normalized to ...
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bioRxiv - Evolutionary Biology 2022Quote: HPLC with mass spectrometry detection was done on a quadrupole LC-MS (Ultimate 3000 UPLC/ISQ EC, Thermo Fisher Scientific) in Single Ion Monitoring (SIM ...