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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for Recombinant Mouse ALCAM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The gel was re-used for protein quantification using SYPRO Ruby Protein Gel Stain (Themo Fisher Scientific, Loughborough, UK) following manufacturer’s instructions and imaged using a UV transilluminator (Ultra-Violet Products Ltd ...
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bioRxiv - Cancer Biology 2023Quote: ... The total amount of protein was quantified using the Qubit Protein Assay Kit and a Qubit Fluorometer (Thermo Fisher). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... 20 mg total protein in 200 ml NLB was precleared with 10 ml protein A Dynabeads (Thermo Fisher Scientific) for 20 min at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Protein content in cell lysates and EVs was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Between 5 and 20 μg of total protein (kept consistent for individual experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of total protein were loaded on NovexTM 10% Zymogram Plus (Gelatin) Protein Gels (Thermo Fisher Scientific: ZY00102BOX) using 2x non-reducing sample buffer (1M Tris-HCl pH 6,8 (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration in the samples was quantified with the Pierce™ BCA Protein Assay Kit (23227, Thermo Scientific) and equal amount of proteins were loaded on the gel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations in the lysates were measured with the Pierce BCA Protein Assay Kit (Thermo Scientific™, CAT: 23227). Lysate mixed (10-25 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein was measured using Pierce BCA Protein Assay Kit according to the manufacture’s microplate protocol (Thermo Fisher 23225). 1X Laemmli sample buffer (Bio-Rad 161-0747 ...
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bioRxiv - Genomics 2022Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop ND1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentrations of solubilized protein precipitates were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Bremen, Germany). For LC-MS/MS ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected and the protein concentration was quantified using bicinchoninic acid protein assay (BCA assay; Pierce, Thermo Scientific). 50 μg of protein lysate was denatured and were run on a 4-12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein concentration was determined using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher #53226). 30 μg of total protein sample was mixed with Laemmli buffer (180 mM DTT (Sigma #D9779) ...
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bioRxiv - Genomics 2023Quote: ... Protein quantification for each fraction was done using manufacturer’s protocol for Pierce 660nm protein assay reagent (Thermo Scientific, USA). Each Fraction was subjected to iCLIP protocol as described in Huppertz et al 201441 using rabbit-CLAMP antibody to immuniprecipitate bound RNAs which were extracted using proteinase K and phenol:chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... Protein bands were visualised by Coomassie blue staining using QuickBlue Protein Stain (LuBio, Zürich, Switzerland) and accompanied by either a SeeBlueTM Plus2 Pre-stained Protein Ladder (Invitrogen) or a PageRuler Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified and proteins were immunoprecipitated using α-FLAG M2 magnetic beads or protein G-conjugated Dynabeads (ThermoFisher) pre-incubated with rabbit α-GFP antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The protein lysates were separated using Bolt 4–12% Bis-Tris Plus Gels (Burlington ...
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bioRxiv - Synthetic Biology 2022Quote: ... The content of protein in the samples was determined with the A280 Total Protein Assay (Thermo Scientific, Wilmington, DE). A volume equivalent to 100 μg protein per sample was taken through a modified filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations of the EV isolates were determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) and 10 ug aliquots were prepared and stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration of the supernatant was then determined using Pierce™ BCA Protein Assay Kit (# PI23225, Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Protein concentrations of all samples were determined using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Results were normalized by total protein concentrations as determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog #23225). PBMC lysates were not diluted for this assay ...
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bioRxiv - Cancer Biology 2023Quote: Bicinchoninic acid (BCA) assay protein concentration determination: The Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, cat no. 23225) was used for protein quantification ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein content in the supernatant was determined using a colorimetric micro–BCA Protein Assay kit (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein served as the loading control by covalently labeling lysine residues (No-Stain Protein Labeling Reagent, Thermo Fisher) following the manufacturer’s protocol and visualized by fluorescence imaging (488 nm) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein abundance was determined using the protein area calculated by the Proteome Discoverer software (Thermo Fisher Scientific; version 1.4).
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bioRxiv - Cell Biology 2024Quote: ... and normalized to protein levels determined in the same lysate by Pierce BCA Protein Assay Kit (Thermo Scientific, USA).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined with the MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific, United Kingdom).
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... Proteins from isolated RGCs were extracted using T-PER™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, Cat.# 78510) supplemented with HALT protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The ladders used were Novex Sharp Pre-Stained Protein Standard and SeeBlue Plus2 Pre-Stained Protein Standard (Life Technologies). The gels were transferred on to a 0.2µm nitrocellulose membrane (Thermo-Scientific ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined with a BCA assay (Pierce™ BCA Protein Assay Kit; #23225, ThermoFisher, Carlsbad, CA, USA). Total protein (5∼15 µg ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA). After mixing 400 μL of supernatant with 400 μL of chloroform and 320 μL of water ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the extract was determined using a BCA protein assay kit (Thermo Fisher Scientific, cat. no. 23225) and adjusted to 2 μg/μL using the IP lysis buffer.
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bioRxiv - Immunology 2023Quote: ... Purified P.ch MSP1 protein and purified P.y MSP1 protein were biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-PE (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of lysates was quantified using the PierceTM Protein Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and measured for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentration in the samples was determined with a BCA assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Microbiology 2024Quote: ... The concentrations of the protein extracts were determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a verification of the protein extracts was carried out on a 12.5% acrylamide SDS-PAGE gel stained with Coomassie brilliant blue R-250 ...
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration of each cell lysate was determined with a BCA protein assay kit (Thermo Fisher, # 23225). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified protein samples were incubated with NeutrAvidin® Agarose resin (50 µL per 1 mg protein, Thermo Scientific) for 2 hours at RT ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein content was quantified using PierceTM BCA protein assay kit as per the supplier’s protocol (Thermo Scientific, USA). For albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl (for ChIP) or 100 μl (for ChIP-seq) Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 3 µg (for ChIP ...