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Citations for Thermo Fisher :
6301 - 6350 of 10000+ citations for Recombinant Human PENK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The supernatants containing total cortex protein lysate were quantified using the Pierce™ BCA Protein Assay kit (ThermoFisher). Immunoblotting was performed using standard protocols with 10μg of protein loaded onto SDS-PAGE pre-cast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein content was quantified using the Pierce bicinchoninic acid (BCA) Protein-Assay kit (23225, Thermo Fisher Scientific Inc.) and using a Mithras LB 940 Multimode Microplate Reader (Berthold Technologies) ...
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bioRxiv - Genetics 2021Quote: ... bound to 50 μL/sample (or 1.5 mg/sample) protein G-coated magnetic beads (Dynabeads Protein G, Invitrogen). The subsequent steps of immunoprecipitation and washes were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein quantification was performed using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, catalog no. 10678484)) ...
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Thermo-Fisher Scientific). Protein samples were sent for trypsin digestion and LC-MS/MS analysis (Aberdeen Proteomics Core Facility (www.abdn.ac.uk/ims/facilities/proteomics/)) ...
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bioRxiv - Immunology 2021Quote: ... The protein contents of the isolated MLBs were determined by a Pierce BCA protein assay kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The protein content of the hypotonic lysates was measured by Micro BCA protein assay kit (Thermo Scientific, 23235) and the free heme levels were expressed per mg of protein ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentrations in BAL fluid were measured by BCA Protein Assay according to the manufacturer’s instructions (ThermoFisher). Turbidity was determined following gentle mixing by diluting 25ul of sample with 75ul DPBS and measuring the optical density of 600nm and multiplying by the dilution factor ...
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bioRxiv - Microbiology 2021Quote: ... The values were normalized with total cellular protein determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Total protein concentrations were determined using the EZQ® Protein Quantitation Kit (Molecular Probes, Inc. Eugene, OR, USA) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using NuPAGE 4-12% precast Bis-Tris protein gels in MOPS SDS running buffer (Invitrogen), transferred onto Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Genetics 2020Quote: Up to 10μg of total protein was resolved on NuPAGE™ 3-8% Tris-Acetate Protein Gels (Invitrogen) in 1x NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was estimated using a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The nitrocellulose membrane and Whatman filters (all GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... and the protein concentration of each sample was measured using the BCA Protein Assay kit (Thermo Scientific #23227) according to the manufacturer’s instructions ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Total protein concentration was determined for cell lysates with the Pierce BCA protein assay kit (Thermo Fisher Scientific), utilizing 50 μg of cell lysate for each sample ...
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bioRxiv - Microbiology 2022Quote: ... Total protein concentration in the resulting supernatant was quantified using a Coomassie (Bradford) Protein Assay Kit (Thermo Scientific) according to the manufacturer’s Standard Microplate Protocol and a BioTek Synergy HT Microplate Reader ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations were measured using the BCA Protein Assay kit according to the manufacturer’s instructions (Thermo Scientific Pierce). Whole protein (30μg/lane ...
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bioRxiv - Cancer Biology 2022Quote: ... Beads were prepared by incubating them in 0.5% BSA in PBS and antibodies overnight (100μL of Dynabeads Protein A or Protein G (Invitrogen) plus 20μL of antibody) ...
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bioRxiv - Developmental Biology 2022Quote: Global novel protein synthesis was assessed using the Click-iT Plus OPP Protein Synthesis Assay Kit (Life Technologies, #C10456 for green fluorescence ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody-DNA complex was precipitated with protein A/G magnetic beads or protein A sepharose beads (Invitrogen). After washing ...
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bioRxiv - Cell Biology 2022Quote: The protein concentration of each fraction was measured using the Pierce BCA Protein Assay Kit (#23227, Thermo Scientific™ ...
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bioRxiv - Cancer Biology 2022Quote: ... Total concentration of proteins in cell lysates was measured by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the recommendations from the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration of the cleared lysate was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). The CatchPoint cAMP ELISA was then performed according to the manual using 0.8 µg protein as input ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration in the supernatant was determined using Pierce 660 nm protein assay (Thermo Fisher Scientific, 22660) and the amounts of protein (~0.5 μg ...
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bioRxiv - Genomics 2022Quote: ... Protein concentration in each fraction extract was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Following separation on precast SDS-PAGE gels (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total protein concentration was assessed using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA), and toxin levels reported as OD450nm/mg total protein ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL).
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bioRxiv - Microbiology 2022Quote: Protein concentrations were determined using a Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) and a Benchmark Plus Microplate Reader (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Protein concentration of all cellular lysates were first determined using the Pierce BCA Protein Assay Kit #PI23227 (ThermoFisher) prior to SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... The amount of protein was determined with the PierceTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Relative protein amount for each sample was determined using the Micro BCA Protein Assay Kit (ThermoFisher; Cat# 23235), and the ELISA readouts were normalized to account for the differences in total protein loaded into each well ...
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bioRxiv - Microbiology 2024Quote: ... and the protein concentration was determined using Pierce™ BCA Protein Assay Kits (Thermo Fisher Scientific, cat# 23225). The lysates were subsequently reduced with 1 × Protein Loading buffer (TransGen ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was measured by Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Rock ford, IL, USA). The peptides mixtures were clean-up to obtain detergent-free samples with the same kit ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein concentration was measured using the Qubit Protein Assay Kit and Qubit 3 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Total protein amounts per lane were stained with No-stain™ Protein Labelling Reagent (Thermo Fisher Scientific; A44449) and used for lane or dot normalization ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and protein concentration was determined by the Pierce BCA protein assay kit (ThermoFisher, 23225). 250μg of each cell lysate were reduced with 5 mM DTT for 1 h at 37 °C and alkylated with 10 mM iodoacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Protein precipitate was solubilized in 8M urea and Bicinchoninic (BCA) protein assay kit (Thermo Fisher Scientific, MA, USA) was used to determine the protein concentrations of each sample.
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bioRxiv - Neuroscience 2022Quote: ... brain lysates were assayed for total protein concentration using Pierce BCA protein assay (Thermo Fisher Scientific, Rockford, Illinois) as per manufacturer protocol (50-fold dilution) ...
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bioRxiv - Bioengineering 2022Quote: ... Sample protein concentration was measuring using a Bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, MA, USA), with bovine serum albumin standards ranging from 20-2000 μg/mL.
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Samples were diluted to 1.2 µg/µl with RIPA buffer and mixed with the respective amount of 6x Laemmli buffer (375 mM Tris pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein Thermal Shift dye and Tris buffer according to the Protein Thermal Shift™ Dye Kit (ThermoFisher Scientific) protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined from cleared lysates with either the PierceTM BCA Protein Assay Kit (Thermo Scientific #23225) or the PierceTM Rapid Gold BCA Protein Assay Kit (Thermo Scientific #A53225PR) ...
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bioRxiv - Biophysics 2024Quote: ... Protein samples were separated using pre-cast 4-12% Bis Tris Protein Gels (Thermo-Fisher Scientific, Waltham MA) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting protein concentrations were determined with a Bicinchonic acid assay (BCA - Pierce BCA Protein Assay Kit, ThermoFisher, Germany) and 20ug of protein were filled up to 140ul with 100mM HEPES pH 8 and transferred to a 96 deep-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... pierce BCA protein assay kit (Cat# 23227) and page ruler protein ladder (Cat# 26620) were purchased from Thermofisher.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the samples were determined with a standard BCA protein assay (Thermo Fisher Scientific, Waltham, USA) and 4 µg of each sample was pipetted into a final volume of 50 µl in AMBIC for the tryptic peptide preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of total extracts was measured by the BCA Protein Assay Kit (Thermo Scientific™, #23225). The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration for each sample was measured using a BCA Protein Assay kit (cat # 23225, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... total protein loading on the PVDF membrane was stained with No-StainTM Protein Labeling reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...