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Citations for Thermo Fisher :
6301 - 6350 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Wells were then incubated with a secondary goat anti-human IgG labeled with horseradish peroxidase (HRP) (1:5000 Invitrogen) TMB substrate (SIGMA) ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen). Biotinylated lectins were precomplexed (on ice ...
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bioRxiv - Molecular Biology 2022Quote: CHO-K1 cells stably expressing human RXFP4 (hRXFP4-CHO) or RXFP3 (hRXFP3-CHO) were maintained in DMEM/F12 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... slides were incubated for 1 hr with goat anti-human 555 (Thermo Fisher Scientific, A-21433; 1:1000 dilution) and goat anti-rabbit 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen human postmortem brain tissues were sectioned at 16-25 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes on dry ice and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Molecular Biology 2023Quote: ... The MS/MS spectra were searched against the target-decoy Human RefSeq database (release Jan. 21, 2020, containing 80,872 entries) in Proteome Discoverer 2.1 interface (Thermo Fisher) with Mascot algorithm (Mascot 2.4 ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Neuroscience 2022Quote: ... conditioned neuronal media was diluted and handled according to the protocol of the Human Aβ42 Ultrasensitive ELISA Kit (Invitrogen) followed by colorimetric readout at 450 nm.
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bioRxiv - Molecular Biology 2022Quote: Human AAA/ control tissue samples were mounted on poly-L-lysine pre-coated SuperFrost Plus slides (Thermo Fisher Scientific). Human OCT-embedded frozen tissue was cut into 8μm thick slides ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (human)-Alexa Fluor 594 (T13343) and Wheat Germ Agglutinin (WGA)-Alexa Fluor 594 (W11262) were obtained from ThermoFisher Scientific (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by washing and addition of 100 µL/well of goat anti-human IgG-HRP conjugate (Invitrogen, 62-8420) at 1:500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was analyzed for gene expression changes using a microarray analysis (Clariom S Human HT, Thermo Fisher Scientific). Each replicate (100ng of total RNA ...
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bioRxiv - Biophysics 2024Quote: The human melanoma cell line MV3 [32] was cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, 11504496) containing L-Glutamine and Sodium Pyruvate ...
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bioRxiv - Biochemistry 2024Quote: ... followed by staining with Alexa Fluor 647-conjugated Goat anti-Human antibody (Thermo Fisher Scientific, A-21445, 1:500).
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bioRxiv - Cell Biology 2024Quote: IMR90 fibroblasts were differentiated to myofibroblasts with 5ng/mL Recombinant Human Transforming Growth Factor -β1 (TGF-β1) (Invitrogen, #PHG9204) for 48h in serum free media ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... a solution of 10-3 mg/ml PE- conjugated anti-human CD62E (clone P2H3, Thermofisher Scientific, #12-0627-42) has been introduced in the chips to stain the endothelium for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... The levels of soluble and insoluble human Aβ40 and Aβ42 in these extracts were quantified using ELISA kits (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of HAs was performed with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen) and incubated on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Genetics 2023Quote: ... 500 ng of PCR products were saturated with 2 μg of unlabeled Cot1 human DNA (Invitrogen, cat. 15279-011) and 0.2 nmol of oligonucleotides corresponding to the universal Illumina adaptors (see above ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Cancer Biology 2024Quote: ... human mammary fibroblast (MF) and cancer associated fibroblast (CAF) were maintained in DMEM/F-12 (Thermo Fisher Scientific, #21331046) supplemented with 2 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 myotubes and biopsy- derived human primary myotubes were transfected with plasmids using lipofectamine 3000 (Thermofisher, Waltham, MA, USA) following the supplier’s instructions.
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney 293T (HEK293T) cells were grown in complete DMEM (Dulbecco’s Modified Eagle’s Medium (DMEM, Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2022Quote: ... at 10ng/ml final concentration along with dynabeads at a ratio of 25µl Human anti CD3/28 labelled Dynabeads/10 million cells (#11132D, Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Genomics 2023Quote: ... cDNAs from the human KZFPs were codon-optimized and synthesized using the GeneArt service from ThermoFisher (former Life Technologies). Sequences were cloned into the doxycycline inducible expression vector pTRE-3HA which yields a C-terminally tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Bioengineering 2023Quote: ... The human dermal fibroblasts were cultured in a reconstituted Complete medium composed of DMEM medium (Dulbecco’s Modified Eagle Medium Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37 °C for 1 hour followed by adding anti-human Alexa Fluor-647 (Invitrogen) secondary Abs for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... for activated cells with human PLK1-specific siRNAs (#4390824, s448) and negative control siRNA (#4390846) (Invitrogen, Waltham, Massachusetts, USA) (150 ng/106 cells) ...
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bioRxiv - Immunology 2023Quote: ... The human embryonic kidney 293T/17cells (ATCC, CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (Gibco Life Technologies) containing 10%FCS ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled RNA were hybridized to Affymetrix Gene Chip Human Gene 1.0 ST arrays (Thermo Fisher Scientific, Meerbusch, Germany, 901086). cDNA syntheses ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to prepare samples and to facilitate hybridization to the human Clariom™ D arrays (Thermo Fisher Scientific). Transcriptome Analysis Console (TAC ...
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bioRxiv - Microbiology 2023Quote: ... the human microbiome database was used as described by Li et al.30 and analyzed using Proteome Discoverer 2.5 (Thermofisher). For subsequent data analysis ...
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bioRxiv - Microbiology 2023Quote: A549 human epithelial cells (ATCC ref# CCL-185) and A549 stable cell lines were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: The cDNA of human ERGIC-53 was subcloned from a cDNA library from HeLa cells and inserted into the pcDNA3.1 vector (Invitrogen). Subsequently ...