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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... After 3 washes Hoechst 33258 nuclear stain (ThermoFisher H3569, 1:1500 for 5 min) was used to counter stain the nuclei ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Developmental Biology 2019Quote: ... with 5 % fetal calf serum (PAN-Biotech) and 1 % Penicillin/Streptomycin (Thermo Fisher Scientific). The human Atoh8 expression plasmid [30] was transfected by electropulsing using the Cell Line Nucleofector System (Lonza ...
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bioRxiv - Bioengineering 2021Quote: ... The composition of 1 L MM2 (pH = 6.9) was 5 g glucose (Acros Organics), 2.5 g of (NH4)2SO4 (Lach-Ner) ...
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bioRxiv - Plant Biology 2021Quote: ... or 1/5 SuperSignal™ West Femto Maximum Sensitivity Substrate (34095, Thermo Fisher Scientific) was done using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... in (i) 300μl MTH buffer along with MitoProbe™ DiIC1(5) (1:300; Invitrogen) or (ii ...
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bioRxiv - Cell Biology 2021Quote: ... and CellMask DeepRed (1:1000 dilution from 5 mg/ml stock solution, Invitrogen C10046) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 1× Halt Protease Inhibitor Cocktail and 5 mM EDTA (Thermo Fisher Scientific). Lysate was clarified by centrifugation and the total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... cells were passaged 1:5 to uncoated Petri dishes by adding trypLE (Life Technologies) for 5 minutes to dissociate the cells before being resuspended in growth medium and plated.
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bioRxiv - Cell Biology 2019Quote: ... counterstained with DAPI (1:2000) for 5 minutes and mounted in ProLong Gold (Invitrogen). Actin was visualized by phalloidin-AF647 staining (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... silencer siRNAs (5 nM) for indicated genes or Negative Control siRNA #1 (Ambion, AM4611) were transfected with Lipofectamin RNAi MAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Triethylamine and 5-hehyn-1-ol 97% were purchased from Acros Organics (Geel, Belgium). Deuterium oxide (D2O ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μM DAF-FM diacetate and 1 μM LysoTracker Red DND-99 (Life Technologies), respectively ...
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bioRxiv - Bioengineering 2022Quote: ... consisting of 5 μL TaqMan Fast Virus 1-Step Master Mix (cat# 4444432, Thermofisher), 1.2 μL forward primer (0.6 μm) ...
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bioRxiv - Microbiology 2022Quote: ... approximately 1 gram of snottite biofilm was preserved in 5 parts RNAlater (Ambion, USA) within 4 hours of collection ...
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bioRxiv - Molecular Biology 2022Quote: ... and high dose RNAse I (Thermo Fisher #AM2295; 1:5 diluted in cold DPBS) or low dose RNAse I (1:50 dilution for neurons ...
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bioRxiv - Cell Biology 2023Quote: ... or TMTpro™ 16 plex Label Reagent Set 1 x 5 mg (ThermoFisher: A44520) for whole-cell protein quantitation ...
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bioRxiv - Immunology 2024Quote: ... or with Deep Red Anthraquinone 5 (DRAQ5, dilution 1:500 in PBST 0,05%; Invitrogen), before mounting with ProLong Gold anti-fade reagent (Invitrogen).
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bioRxiv - Genetics 2023Quote: ... samples were washed and incubated in 1:10000 DAPI (Invitrogen, D1306, 5 mg/mL) for 5min ...
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bioRxiv - Immunology 2023Quote: ... TriLink) and all four nucleotides with 1-methylpseudouridine-5’-triphosphate (m1ΨTP; Thermo Fisher Scientific) substituting for uridine-5’-triphosphate (UTP ...
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bioRxiv - Neuroscience 2023Quote: ... and DAPI (D1306, Thermo Fisher Scientific, 1:500 from a 5 mg/ml stock). All reactions were performed in FACS buffer and incubated for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 hour of blocking with normal goat serum (NGS) (5%, Invitrogen, #10000C). Primary antibodies raised against NaV1.1 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 45 min and 1% formaldehyde for 5 min (Thermo Scientific 28908). The reaction was quenched with 0.125 M glycine ...
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bioRxiv - Pathology 2023Quote: The cDNA was then diluted by 1:5 in nuclease-free water (#10526945, ThermoFisher). qPCR was performed using 2x PCRBIO Sygreen Blue Mix Hi-ROX (#PB20.16-20 ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblotted with anti-ubiquitin antibody 1:500 in 5% BSA TBST (13-1600, Invitrogen) and reprobed with anti-C-cadherin antibody 1:100 in 5% non-fat milk TBST (Developmental Studies Hybridoma Bank ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... cells were incubated for 5 minutes with CellMask orange (1:40000, Thermo Fisher Scientific) and 5 minutes with SPY650 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-Keratin 5 (1:200; clone EP1601Y; MA5-14473; RRID:AB_10979451; Thermo Fisher Scientific), rabbit anti-SFTPC (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% FBS and 1% penicillin-streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 hour in blocking solution containing Hoechst 33342 (Invitrogen; 5 µg/ml) and secondary antibodies labeled with AlexaFluor 488 or AlexaFluor 555 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 minutes before treating with 1 µM of 5-Ph-IAA (Fisher Scientific) for 2h and harvesting for immunoblotting as well as ChEC-seq2.
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bioRxiv - Neuroscience 2024Quote: ... larvae at 5 dpf were incubated in 3 µM FM 1-43 (Thermofisher, T3163) in E3 media for 35 s ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-AIM2 antibody (MA5-38442, 1:1000 in 5% BSA, Thermo Fisher Scientific). Following primary antibody incubation overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... and PASH-1::GFP was co-IP’d with 5 ug GFP antibody (Invitrogen, A11120) for 90 minutes at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
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bioRxiv - Immunology 2019Quote: ... in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP) or 1 ml (48WP) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...
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bioRxiv - Microbiology 2020Quote: ... The UPLC system consisted of 0.1% formic acid (mobile phase A) in ultrapure water and a mobile phase B consisting of 0.1% formic acid (Fisher Scientific, Loughborough, UK) in acetonitrile ...
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bioRxiv - Genetics 2022Quote: ... FRAP was performed on 3T3-L1 preadipocytes transiently transfected with GFP-tagged CIDEC were incubated with 200 μM of oleic acid and stained with Bodipy 558/568 C12 fatty acid (Molecular Probes, D3835) for 15 hours ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were then stained for 1 minute in 5% Phosphomolybdic/Phosphotungstic acid solution and immediately transferred to 2.5% light green SF yellowish in 2.5% acetic acid (Fisher Scientific, cat# A38S-500) for 4 minutes ...
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bioRxiv - Microbiology 2024Quote: Total nucleic acids were extracted from 200 μL of inoculated viral transport medium using the MagMax™ Express 96 Nucleic Acid Extractor (Applied Biosystems) and the MagMax Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s minimum essential medium supplemented with 20% heat-inactivated fetal bovine serum and 1% non-essential amino acids (Gibco, Waltham, MA), and cultured in 10% CO2/90% air ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H2SO4) and the optical density was measured at a wavelength of 450 (OD450) (MULTISKAN FC, Thermo scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain, Thermo Scientific). Each lane from the gel was cut and placed in 1.5 ml tubes ...
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bioRxiv - Immunology 2022Quote: ... L981F amino acid changed compared to WA-1) or S variant genes (Wang et al., 2021a) using Lipofectamine 3000 transfection reagent (ThermoFisher, CA). Forty-eight hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... the gels were fixed by 40% ethanol/10% acetic acid for 1 hr and stained overnight in 200 mL of Krypton Fluorescent gel stain (Life Technologies) diluted 1/10 in water ...
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bioRxiv - Cell Biology 2019Quote: ... The gel was fixed with 40% methanol/10% acetic acid and then stained for 1 h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). After in-gel digestion ...
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bioRxiv - Biophysics 2019Quote: ... 0.1% formic acid) gradient over 205 min and analyzed using an EasySpray source and Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). Wide isolation single ion monitoring (WiSIM ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...