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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al ...
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bioRxiv - Molecular Biology 2023Quote: ... or 5 mM dimethyl succinate (SUC, CAS # 150731000, Thermo Fisher Scientific, New Jersey, USA) for the indicated times.
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bioRxiv - Molecular Biology 2022Quote: ... followed by selection with 10 µg/ml blasticidin or 5 µg/ml puromycin (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Plates were then blocked with a 5% powdered milk phosphate buffered saline (PBS) (ThermoFisher) solution for at least 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.25 μl of Dream taq DNA polymerase (5 U/μl, Thermo scientific lab) in deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen) for 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl were injected using a Vanquish Horizon UPLC (Thermo Fisher Scientific; Waltham, MA) and compounds were separated on a Zorbax Eclipse Plus C18 guard (2.1 × 50 mm and 1.8 μm particle size ...
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bioRxiv - Cell Biology 2023Quote: ... The following siRNA target sequences were used: GTPBP5 siRNA: 5’-CGGUGGACACGUCAUUCUGTT-3’ (134621, Ambion); GTPBP7 siRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Amplification was performed on a QuantStudio™5 Real-Time PCR System (ThermoFisher Scientific) using the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated in blocking buffer (5% goat serum (Gibco, ThermoFisher Scientific, Waltham, MA) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid pIP/VSV-G (5 µg) (ViraPowerTM Lentiviral Packaging Mix, Thermo Fisher Scientific), 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Biophysics 2023Quote: ... at 37 °C and 5% CO2 in DMEM medium with L-glutamine (ThermoFisher, 11965092) containing 10% FBS (BI ...
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bioRxiv - Cell Biology 2023Quote: ... cells were differentiated for 5 days in media consisting of DMEM (Gibco #11995-065) supplemented with 1% v/v penicillin-streptomycin (Gibco #15140-122) ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were stained with 5 nM CellTrace™ Violet Cell (C34557, Thermo Fisher) for 8 minutes in 37L°C water bath ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Gibco, ThermoFisher Scientific, Waltham, MA, USA), 1% L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... stained with DAPI 5 min and mounted using Prolong Diamond Antifade Mountant (Invitrogen, P36961). Cells were examined as described above.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 5% FBS (HyClone) ...
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bioRxiv - Developmental Biology 2023Quote: ... The backbone medium for the first 5 days of differentiation was BE1: MCDB131 (Gibco) supplemented with 0.8 g/L cell-culture-tested glucose (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 hours and 5 hours using a Floid Cell Imaging Station (Thermo Fisher Scientist), and then manually counted for quantification ...
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bioRxiv - Developmental Biology 2023Quote: ... 5–6 trophectodermal cells were biopsied and their ploidy was assessed by Thermo Fisher Scientific’s NGS technology ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 g/L (34 mM) L-glutamine (Gibco, 200mM, cat. no. 25030-024) – hereafter called “imaging medium” ...
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bioRxiv - Biophysics 2023Quote: ... The cell suspension was taken up in 5 mL of pre-warmed DMEM (Invitrogen) containing 10% fetal calf serum (FCS ...
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bioRxiv - Developmental Biology 2023Quote: ... Monocyte differentiation was induced from HSC for 5-7 days in RPMI (Gibco, #11875093), 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... The thinning was conducted by the automated milling software AutoTEM 5 (Thermo Fisher Scientific) in a stepwise manner from current 0.5 μA to 30 pA at 30 kV ...
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bioRxiv - Biochemistry 2023Quote: ... The 5’-capped transcripts were synthesized in vitro using mMessage mMachine T7 (ThermoFisher Scientific), followed by incubation with DNase for 15 min at 37°C to remove the template DNA ...
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bioRxiv - Neuroscience 2023Quote: ... was used to perform real-time PCR on a QuantStudio 5 thermocycler (Applied Biosystems). The abundance of each transcript was normalized to GAPDH as ΔΔCt ...
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bioRxiv - Microbiology 2023Quote: qRT-PCR was performed using QuantStudio 5 real-time PCR System (Thermo Fisher Bioscientific) and Luna Universal one-step qRT-PCR kit (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were maintained at 37°C and 5% CO2 in DMEM (ThermoFisher) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... 5 μL of cDNA were mixed with High-Fidelity Platinum PCR Supermix (Thermo Fisher) containing 50 nM forward primers specific for the framework region 1 of VH1 ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Guanosine-5-diphosphate (GDP) and unlabeled GTPγS were purchased from Fisher Scientific (Waltham, MA) and Sigma-Aldrich (St ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Microbiology 2023Quote: ... Host cell nuclei were stained with Hoechst 33342 (5 μg/ml, Thermo Fisher Scientific) for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were assembled with a 5′ cytomegalovirus (CMV) promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of glutathione magnetic agarose beads (Pierce Glutathione Magnetic Agarose Beads, Thermo Fisher) were equilibrated with wash buffer (100 mM sodium phosphate pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in 5 µl ProLong™ Diamond Antifade Mountant solution (Thermo Fisher). The mounting solution containing stained yeast or hyphae was transferred to glass slides and covered with a coverslip ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 5 µg/ml NeutrAvidin (Thermo Fisher Scientific), then blocked in PBS containing 1% BSA for 1 hour at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated in blocking buffer (5% goat serum (Gibco, ThermoFisher Scientific, Waltham, MA) in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... containing 5 µL Fast SYBR Green Master Mix (Applied Biosystems by Thermo Fisher Scientific), 500 nM reverse and forward primers designed to amplify 80–120 bp of the genes of interest ...
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bioRxiv - Plant Biology 2023Quote: ... containing 5 µL Fast SYBR Green Master Mix (Applied Biosystems by Thermo Fisher Scientific), 500 nM reverse and forward primers designed to amplify 80–120 bp of the genes of interest ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 45 min and 1% formaldehyde for 5 min (Thermo Scientific 28908). The reaction was quenched with 0.125 M glycine ...
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bioRxiv - Pathology 2023Quote: The cDNA was then diluted by 1:5 in nuclease-free water (#10526945, ThermoFisher). qPCR was performed using 2x PCRBIO Sygreen Blue Mix Hi-ROX (#PB20.16-20 ...
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bioRxiv - Immunology 2023Quote: ... and bound fractions were eluted with 5× Lane Marker Reducing Sample Buffer (Thermo Fisher). For detection of biotynilated DNA in the IP products ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblotted with anti-ubiquitin antibody 1:500 in 5% BSA TBST (13-1600, Invitrogen) and reprobed with anti-C-cadherin antibody 1:100 in 5% non-fat milk TBST (Developmental Studies Hybridoma Bank ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts of cDNA and 5 μL of SYBR green master mix (Thermo Scientific). qPCR was performed on a Lightcycler 480 ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR was performed with 5 µL 2x SYBR Green PCR Master mix (Applied Biosystems), 1 µL ChIP DNA ...
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bioRxiv - Microbiology 2023Quote: ... and were cultured at 37°C and 5% CO2 in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... aspiration of supernatant and resuspension in 5× Lane Marker Reducing Sample Buffer (Thermo Fisher). Samples were then subjected to SDS-PAGE followed by immunoblot analysis with the indicated antibodies ...
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bioRxiv - Immunology 2023Quote: ... The resin was then treated with 5× Lane Marker Reducing Sample Buffer (Thermo Fisher). To generate the Triton X-100-soluble and -insoluble fractions ...