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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... and the Qubit dsDNA BR Assay Kit was used to determine its amount (Invitrogen, Catalogue number: Q32850). The DNA samples were delivered to Novogene ...
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bioRxiv - Cancer Biology 2024Quote: ... Predesigned TaqMan assay (Polq Assay ID: Mm00712819_m1) and TaqMan gene expression master mix (Applied Biosystems Assay ID# 4369016) were used for quantitative real-time RT-PCR ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation of GFP-tagged DSB-1 was performed with the same amount of lysate determined by BCA kit (Pierce BCA protein assay kit #23225, Thermo Scientific, Waltham, Massachusetts, USA) from wild type and pph-4.1 RNAi animals ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were determined using a BCA kit according to the manufacturer’s protocol (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, IL, USA). Citrate synthase (CS ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cleaned with the Qiagen PCR Purification Kit (Valencia, CA) and quantified using a Qubit fluorometer (dsDNA HS Assay Kit, ThermoFisher Scientific, Grand Island, NY). Samples were diluted in 10 mM Tris buffer (pH 8.5 ...
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bioRxiv - Systems Biology 2020Quote: ... Nucleic acid extracts were quantified with the Quant-iT™ HS ds-DNA assay kit and the Quanti-iT™ RNA HS kit with a Qubit™ fluorometer (Invitrogen, USA).
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bioRxiv - Pathology 2021Quote: ... kept on glass slides overnight at 45°C and then in situ hybridization was performed using the Affymetrix ViewRNA ISH Tissue Assay kit (QVT0050) and ViewRNA Chromogenic Signal Amplification kit (QVT0200) (Thermo Fisher Scientific, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd). The filtrate obtained from untreated SM was designated as SM_NT and the filtrate obtained from untreated IMF was designated as IMF_NT.
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bioRxiv - Biophysics 2021Quote: ... We experimentally determined the precise membrane position using DiD membrane staining dye (Thermo Fisher, D7757). We found that after thresholding the 488ex channel image by Li’s algorithm and then eroding the binary mask by 3 px ...
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bioRxiv - Microbiology 2020Quote: ... DNA was transferred to positively charged nylon membrane (BrightStar-Plus Positively Charged Nylon Membranes; Invitrogen) using a Semidry Electroblotting System (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were transferred to a nitrocellulose membrane via the iBlot™ Gel Transfer Device (Invitrogen). Membranes were blocked overnight with 3% BSA (ALB001.100 ...
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bioRxiv - Immunology 2020Quote: ... and quantified by the Bicinchoninic Acid Assay (BCA) assay (Thermo Fisher). Equivalent masses (15ug ...
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bioRxiv - Neuroscience 2020Quote: ... Actin assay Act5C (assay ID Dm02361909_s1) was acquired from Thermo Fisher Scientific.
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bioRxiv - Genetics 2021Quote: ... All qPCR assays used were TaqMan Gene Expression Assays (Life Technologies). Ct values for each gene were normalized to the house keeping gene GAPDH ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined using the Pierce assay 660 assay (ThermoFisher). 1 mg of protein was used for each condition and brought up to a total volume of 1 ml in IP buffer ...
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bioRxiv - Immunology 2023Quote: ... and quantified using Pierce Bicinchoninic Acid Assay assay (Thermo Fisher, 23225). When indicated ...
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bioRxiv - Immunology 2024Quote: ... A bicinchoninic acid assay (BCA) protein assay (Thermo Fisher Scientific, PI23235), was used to quantify protein and 10 to 40 μg total protein were loaded into 10% SDS-polyacrylamide gels and transferred onto 0.22 μM polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial membrane potential of the sperm samples was assessed with the JC-1 fluorescent probe using the kit “MitoProbe™ JC-1 Assay Kit for Flow Cytometry (M34152, Invitrogen by Thermo Fisher Scientific). Diluted sperm in SFMM was incubated for 15 min at 20 °C with JC-1 at a final concentration of 0.5 µM ...
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bioRxiv - Cell Biology 2020Quote: ... resolved proteins were transferred to nitrocellulose membrane (Amersham Biosciences) and immunoblotting was performed using the Western Breeze Chemiluminescence Anti-Rabbit kit (Invitrogen) and anti-GFP polyclonal antibody (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were incubated in reaction mix prepared from the highly sensitive SuperSignal West Dura extended substrate kit (Thermo scientific, 34075), and chemiluminescent signals were recorded using ImageQuant RT ECL Imager (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion) and primers T7_nsiR6_probe fwd/rev and T7_nblA_probe fwd/rev as described previously (79) ...
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bioRxiv - Physiology 2024Quote: ... The samples were filtered through a silicon membrane (1 μm retention limit, Microplastic Sample Preparation Kit, Thermo Fisher Nepean, ON) using glass vacuum filtration funnel ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed after secondary antibody incubation before protein detection using SuperSignal West Pico Chemiluminescence Detection kit (Thermo Fisher Scientific). Images were acquired with a Bio-Rad Chemidoc XRS+ gel imaging system and analyzed with ImageJ/Fiji software (National Institutes of Health) ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were then processed using stringency washes and developed using the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoFisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Biophysics 2021Quote: ... 2005) at 280nm and TCF7L2 proteins concentration was measured using the Pierce™ BCA protein Assay Kit (ThermoFisher) ion-exchange-ninhydrin analysis performed by Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound UHMW DNA was resuspended in the elution buffer and quantified with Qubit dsDNA assay kits (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained by the Click-iT Plus EdU Pacific Blue Flow cytometry assay kit (Thermo fisher, #C10636) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU was detected using the Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit (Thermo Fisher). For the experiments in Figure 2C-D ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The concentration was measured with the Qubit 3.0 Fluorometer and the Qubit dsDNA HS Assay Kit (Thermo Fisher), and adjusted to a final concentration of 10 ng/μl ...
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bioRxiv - Cell Biology 2020Quote: ... Protein quantification of lysates was done with the Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific, #23235) and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: The protein concentration of lysates was measured using the Pierce Coomassie Bradford protein assay kit (Thermo Fisher Scientific). Final protein concentrations were adjusted to 13 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007 ...
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bioRxiv - Cell Biology 2020Quote: ... Sample protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) and the samples were normalized and diluted to 5 µg protein/10 µl with 3x Laemmli sample buffer (30 % glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining cells were quantified by means of fluorescent labelling (CyQuant Cell Proliferation Assay Kit; Molecular Probes, #C7026). The adherent cell fraction (ACF ...
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bioRxiv - Cell Biology 2020Quote: Estimations of protein concentrations were made using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat# 23225). Cells were lysed for Western blot analysis in 2.5% (w/v ...
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bioRxiv - Genetics 2021Quote: ... at a 1:1 (v:v) ratio and quantified using the Quant-iT PicoGreen dsDNA Assay kit (P11496, Invitrogen). 0.15 ng of cDNA in a 1.25 μl volume was used as input for Nextera XT (FC-131-1024 ...
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bioRxiv - Genetics 2021Quote: ... The concentrations of the libraries were estimated using Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, #Q32851). Quality assessment and library size estimation of the individual libraries was done using an HS DNA kit (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... the library with adaptors was determined with the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher, USA) and assessed using the Agilent 2100 Bioanalyzer system assay ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μl was used to quantify DNA concentrations using a Qubit High-Sensitivity DS DNA Assay kit (Invitrogen; Q32851).
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bioRxiv - Developmental Biology 2021Quote: ... and size distribution of the DNA libraries was analysed using High-sensitivity Qubit dsDNA Assay Kit (ThermoFisher, Q32851) and Agilent 2200 Tapestation ...
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bioRxiv - Microbiology 2021Quote: ... RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, USA). RNA integrity was assessed using the RNA Nano 6,000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ISH on formalin-fixed paraffin-embedded sections was performed using ViewRNA ISH Tissue Assay kit (Thermo Fisher Scientific). Gene-specific probe sets for human PIWIL4 (VA1-3014459VT) ...
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bioRxiv - Developmental Biology 2020Quote: Protein concentration for all samples was quantified by Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). All samples were processed for analysis on SDS-PAGE ...
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bioRxiv - Genomics 2020Quote: ... The concentration of each protein extract was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For fractionation ...
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bioRxiv - Genomics 2020Quote: ... The final concentration of DNA was quantified using the Qubit 3.0 Fluorometric dsDNA BR assay kit (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... RNA quantity was re-verified with a Qubit 2.0 Fluorometer using the RNA HS Assay Kit (Invitrogen, Q32855). The three biological replicates with the most consistent RNA yield across conditions were then used for library preparation and sequencing.
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bioRxiv - Immunology 2022Quote: ... TUNEL stainings of lymph node sections were carried out using the Click-iTTM Plus TUNEL assay kit (Invitrogen). Tissue sections were treated with 2 % H2O2 in methanol for 20 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Samples were assessed for quantity with the Qubit™ dsDNA BR Assay Kit (Life Technologies, Carlsbad, CA, USA). All samples (>2.0µg ...
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bioRxiv - Neuroscience 2021Quote: ... RNA concentration was measured using Qubit RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl of each sample was quantified using the Quant-iT High-Sensitivity dsDNA Assay Kit (Thermo Fisher). The quantity of cDNA for one replicate sample of WT 28°C was too low and was excluded from further experiments ...