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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... ODCs were first generated by dissociating Day 6-8 PPTO.46 PDOs with TrypLE (Gibco) dissociation solution for 30-40 minutes in an incubator ...
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bioRxiv - Microbiology 2023Quote: ... The AePer50 qPCR was performed on a QuantStudio 6 Real-Time PCR System (Applied Biosystems), with cycling conditions as follows ...
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bioRxiv - Bioengineering 2023Quote: ... for PEG-RGDS and 6-8 kDa MWCO dialysis membrane for PEG-PQ-PEG (Thermofisher), then lyophilized ...
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bioRxiv - Neuroscience 2023Quote: ... inside 6-well plates with culture medium containing 78% Neurobasal-A without phenol red (Invitrogen), 20% heat-inactivated equine serum (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on a QuantStudio 6 Real-Time PCR System (Thermo Fisher). Primers used in Real time-qPCR experiments are listed in Supplementary Table S2 ...
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bioRxiv - Physiology 2023Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II ...
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bioRxiv - Cell Biology 2023Quote: ... 6 × 105 cells/ml were plated in a 90 mm petri dish (101R20, Thermo Scientific). Cells were treated with LLOMe for 30 minutes at a concentration of 1 mM by dilution in RPMI medium ...
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bioRxiv - Neuroscience 2023Quote: ... The reaction was performed in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) or the StepOne Plus Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: The cell wall stressor assays were done using 6-well plates (Invitrogen, Waltham, MA, USA). The wells were filled with GMM or SMM supplemented with increasing concentrations (20 ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II ...
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bioRxiv - Neuroscience 2024Quote: ... Bound cytokine was detected using a biotinylated IL-6 detection antibody (ThermoFisher Scientific, MP5-32C11), streptavidin horseradish peroxidase (strep-HRP) ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was flowed over 6 ml of washed Ni-NTA resin (ThermoFisher Scientific) in a gravity column ...
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y cells were plated (100,000 cells/well) on 6-well plates (ThermoFisher Scientific, 353046) and allowed to adhere for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... on 6-well plates coated with Gibco Geltrex hESC-qualified Basement Memebrane (ThermoFisher cat #A1413301) using previously established maintenance protocols97 ...
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bioRxiv - Cell Biology 2024Quote: ... For qPCR, purified RNA was reverse transcribed (Thermo, 4368814) and quantified (Applied Biosystems, Quantstudio 6) with SYBR green reagent (Thermo ...
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bioRxiv - Biochemistry 2024Quote: ... then harvested by centrifuging at 4,000 ×g (Fiberlite F9-6×1000 LEX rotor, Thermo Scientific) for 10 min and resuspended with HBS buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... 6 pmol of siRNA (siCHC-1: Silencer Select s475, siCHC-2: s477 Thermo Fisher 4390824) were mixed with 100 µl of OptiMEM reduced serum media (Thermo Fisher 31985070 ...
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bioRxiv - Molecular Biology 2024Quote: ... arteries were incubated for 6 minutes in collagenase type IV (1 mg/mL, Life Technologies) and porcine pancreatic elastase (1 U/mL ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and run on a QuantStudio 6 instrument (Thermo Fisher Scientific). Serial dilutions of RNA standards with known copy numbers were run in parallel in each run to calculate RNA copy numbers in the standard.
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bioRxiv - Biochemistry 2024Quote: ... then harvested by centrifuging at 4,000 ×g (Fiberlite F9-6×1000 LEX rotor, Thermo Scientific) for 10 min and resuspended with HBS buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies), using TaqMan™ Universal Master Mix II (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: Due to the lack of aromatic amino acids within the small protein His6-sP26 the Pierce™ BCA protein assay kit (Thermo Fisher Scientific™, Waltham, USA) was used to determine the concentration of all proteins and was performed according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted for 4 seconds at 4 °C in 100% humidity and plunge-frozen in liquid ethane by using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2019Quote: ... Collected samples were analyzed using native gel electrophoresis (NativePAGE 4-16% Bis-Tris Gel) or denature gel electrophoresis (SDS-PAGE, Novex 4-20% Tris-Glycine Mini Gels, Thermo Fisher Scientific). For SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... of HLC protein extract in 4 × Sample Loading Buffer (Li-Cor, 928-40004) were loaded onto NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, NP0326BOX). Following resolution ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Samples equivalent to 100 μg of DNA were incubated overnight at 4°C with 4 μg of rabbit polyclonal anti-ATF7IP (Invitrogen, PA5-54811), rabbit monoclonal anti-JUNB (C37F9 ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We plated 4*105 cells/well of isolated cardiomyocytes to fibronectin (cosmo bio SFN01)-coated 4 well chamber slides (Thermo Fisher 154526) and cultured them in a 5% CO2 incubator ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were centrifuged at 17,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific) containing 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system, Thermo Fisher Scientific), and cultured in the 20 mM sodium acetate-supplemented culture medium (10% FBS) ...
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bioRxiv - Biochemistry 2021Quote: Images displayed in Figure 4 and negative stain images in supplementary Figure 4 were acquired using a Tecnai TF20 electron microscope (Thermo Fisher, USA) equipped with FEG source operating at 200 keV ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified eIF1A derivatives were labeled at 4 °C with 4-fold excess of Alexa Fluor™ 555 C2 maleimide (Alexa555, Thermo Scientific) following the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... or corresponding isotype control antibodies diluted in FACS buffer for 30 min at 4 ° After fixation and permeabilization for 30 min at 4 °C using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), resuspended cells were kept overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The neuronal cultures were washed twice for 10 minutes with HBSS and then loaded with 4 μM of Fluo-4 AM (Molecular probes, F14201) in HBSS having 0.002% Pluronic F-127 for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the grid was blotted for 4 sec under 100% humidity at 4°C and then plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... Then the cells were stained with a solution of 4 mM calcein AM (TRC, #C125400) and 4 mM ethidium homodimer-1 (Thermo Fisher, #L3224) dissolved in PBS to identify live and dead cells ...
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bioRxiv - Cell Biology 2023Quote: ... The pellets were washed twice and cell surface AF488 fluorescence was quenched by incubation for 30 min at 4 °C with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher Scientific). The difference between the signals from the cells incubated in the absence and presence of anti-AF488 IgG corresponded to apoA-I bound to the cell surface.
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bioRxiv - Immunology 2023Quote: ... the C-terminal FLAG-tagged WT sCTLA-4 and Y139A sCTLA-4 in pCMV-Tag4A were sub- cloned into the pFastBac vector (Thermo Fisher Scientific) to generate pFastBac-WT_sCTLA- 4-FLAG and pFastBac-Y139A_sCTLA-4-FLAG.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 20,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific). For SDS-PAGE ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... and the airway and vessel-free parenchyma was further minced 4 x 4 mm pieces and cryopreserved in high glucose DMEM GlutaMAXTM (Thermo Fisher Scientific) containing 10% DMSO (Carl Roth ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μl of this mixture was loaded into the lanes of a precast 4-12% gradient polyacrylamide gel (4–12% Bolt Bis-Tris Plus Gels, Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: Cells were grown on 1.5mm coverslips coated with matrigel (iMGLs). Cells were first fixed in a 4% formaldehyde (Electron Microscopy cat. # 15714) solution in 4% sucrose in PBS (Thermo Fisher Scientific) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 4 μl of 5 mg/ml linear acrylamide (Ambion), 600 μl of preheated (65°C ...