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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 0.5 μg was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA). qPCR was performed with the SYBR Green PCR Master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... each reverse transcription was performed with 0.2 mg RNA using the High-Capacity RNA-to-cDNA kit (Applied Biosystems # 4387406), in a final volume of 20 µL ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed with RNA standards synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by RT-PCR from the SARS-CoV-2 and 229E viral RNA ...
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bioRxiv - Immunology 2023Quote: ... 1-2 µg of RNA was reverse-transcribed using the High-Capacity RNA-to-cDNA kit according to the manufacturer’s instructions (Applied Biosystems). qPCR was performed to quantify lung RNA levels using the mastermix QuantiTech Probe PCR kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... Quantitative conversion of RNA to single-stranded DNA was executed using a high Capacity cDNA Reverse Transcription kit (Applied Biosystems). Reaction mixtures were prepared using FastStart TaqMan Probe Master Mix (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... The two pooled mixtures were fractionated into four fractions using PierceTM High pH reverse-phase fractionation kit (Life Technologies, 84868), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1000-500 ng of RNA were converted to cDNA with the High-Capacity RNA-to-cDNA kit (Applied biosystems, 4388950) and qPCR was performed on StepOne Real Time PCR System with Taqman Assays (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was converted to cDNA using a High-Capacity cDNA Reverse Transcription Kit following the manufacturer’s protocol (Applied Biosystems; 4387406). The qPCR was performed using Taqman™ Universal Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, United States). RT-qPCR was performed in a Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Purified RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, United States). RT-qPCR was performed in a Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was subsequently separated into five fractions on Pierce High pH Reversed-Phase spin column kit (Thermo Fisher Scientific) using stepwise elution with 0.1% aqueous trimethylamine solution containing 10% to 50.0% of acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... rpsJ or rpsS CDS (IDT) were used as DNA templates for in vitro transcription with MEGAshortscript T7 High Yield Transcription Kit (Invitrogen). The transcripts were dephosphorylated with calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using the High Capacity cDNA Reverse Transcription Kit with RNAse Inhibitor and random primers (Applied Biosystems) under manufacturer’s recommended conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA was synthesized using 0.5 μg of RNA using the High Capacity cDNA Reverse Transcription kit (Life Technologies, 4368814). The qPCR reaction was set up with 1 μl of diluted complementary DNA and 20X TaqMan probes and TaqMan Universal PCR Master Mix (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were synthesized using the High-Capacity cDNA Reverse Transcription Kit with the addition of a RNase Inhibitor (Applied Biosystems). All primers used for qPCR were tested for specificity and sensitivity ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... and the cDNAs were synthesized by reverse transcriptase (RT) using the high capacity cDNA archive random priming kit (Applied Biosystems) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems™, Catalog No. 4374967) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... 2 μg of total RNA was used to synthesise cDNA using the High-Capacity RNA to cDNA Kit (Applied Biosystems). The primer sequences were generated using the primer BLAST software (Basic Local Alignment Search Tool)
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bioRxiv - Physiology 2023Quote: ... Reverse transcription was performed with 1µg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, USA). qRT-PCR was performed on an ABI PRISM 7500 (Applied Biosystems ...
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bioRxiv - Physiology 2023Quote: ... 500 ng of RNA was used as a template to synthesize cDNA (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems). QPCR reactions were carried out using 2X SYBR green (Cowin Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 0.5 µg RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Foster City, CA) in 10 µl reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was made from 1 ug of RNA/sample using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368814). Each Ct value was measured using Lightcycler 480 (Roche ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... The cDNA was prepared from total RNA sample using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA) and it was then amplified with TaqMan gene expression assays or custom primers and normalized to GAPDH using Applied Biosystems 7500 or Rotor-Disc 100 system ...
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bioRxiv - Plant Biology 2023Quote: ... Complemen-tary DNA (cDNA) was synthesized using the High-Capacity RNA-to-cDNA Kit (Applied Biosystems-Thermo Fisher, Schwerte, Germany) and stored at -20 °C ...
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bioRxiv - Physiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from 1 µg of total RNA using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368813). Quantitative real-time PCR was performed using Itaq Universal SYBR green master mix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... And 1µg of total RNA was used to synthesize cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 1 μg of total RNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). qPCR was performed using a ViiA7 system (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Extracted RNA samples were then reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Life Technologies, CA). The resulting cDNA products were amplified by qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... then normalized RNA was transformed into cDNA using the High-capacity cDNA reverse transcription kit (43-688-14, Applied Biosystems). QPCR was performed on a 7900HT fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Amount of total RNA was quantified by spectrophotometry and 0.8 µg total RNA was reversely transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), with the following PCR conditions ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of total RNA was reverse transcribed using High-Capacity cDNA reverse transcription kit (Thermo Fisher scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Briefly guide RNAs used to generate the 19 kb deletion and cloned in the pDG458 plasmid were in-vitro transcribed using the TranscriptAID T7 High Yield Transcription Kit (ThermoFisher) and purified using GeneJET RNA purification kit (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... Complemen-tary DNA (cDNA) was synthesized using the High-Capacity RNA-to-cDNA Kit (Applied Biosystems-Thermo Fisher, Schwerte, Germany) and stored at -20 °C ...
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bioRxiv - Genomics 2023Quote: ... cDNA was synthesized from 1-2 μg total RNA with the Multiscribe High-Capacity cDNA Reverse Transcription Kit (ThermoFisher 4368814).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A reverse transcription (150 ng of total RNA per sample) with random hexamers was performed with the High capacity cDNA Reverse Transcription Kit (#4368814, ThermoFisher) for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was converted to single stranded cDNA by reverse-transcription using the High-Capacity RNA-to-cDNA Kit (Applied Biosystems). qPCR analysis was performed using SYBR Green master mix (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and the following cDNA synthesis was performed with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, # 43-688-13), both according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed with 500ng of RNA using the High-Capacity cDNA Reverse Transcription Kit (Cat: 4368814, Applied Biosystems). Gene expression was assayed by quantitative PCR using TaqManTM Master Mix II no UNG (Cat 4440049 ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted using Trizol and converted to cDNA using a high-capacity cDNA Reverse Transcription kit (Applied Biosystems 4368814). Quantitative PCR was performed using a PowerUp SYBR Green Master Mix (Applied Biosystems A25742 ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified RNA (1 μg) was used for reverse transcription using the High-Capacity cDNA Reverse Transcription kit (4368814, Applied Biosystems). Complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared according to manufacturer’s instructions from 2 ug total RNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems). qPCR reactions were performed in a 384-well format using GoTaq qPCR Master Mix (Promega ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The mixture of TMT-labeled peptides was acidified and fractionated using a high pH reversed-phase peptide fractionation kit (ThermoFisher) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... cDNA from lysate for titering or qPCR was generated using the High Capacity First Strand Synthesis Kit (Applied Biosystems, 4368814) with random hexamers according to the manufacturer’s protocol using a final lysate concentration of 10% of the reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... RNA was converted to cDNA using a high -capacity cDNA reverse transcription kit according to the manufacturer’s protocol (Applied Biosystems). Expression of csuAB pili gene expression by RT-qPCR was performed with cDNA using the qPCR primer pairs listed in Table S1 and following the protocol for Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
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bioRxiv - Genomics 2023Quote: ... 2 μg of total RNA reverse transcribed using High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA, Cat No. 4368814) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (typically 1000 ng) was reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Waltham, MA, USA). The amplified cDNA was diluted to 10 ng/μl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Waltham, MA, USA). The amplified cDNA was diluted to 10 ng/μl ...