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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Myofibroblasts were grown on uncovered polystyrene culture plates in 16% FCS in DMEM supplemented with 1% Penicillin-Streptomycin-L-Glutamine and subcultured at a 1:3 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... cryoprotected in 30% sucrose solution (for 1 to 3 days) and incubated with AF488 streptavidin (1:1000, Invitrogen, Cat#: S11223, 2390711) in PBS containing 0.3% Triton X-100 (Aladdin ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011). After 1 hr incubation ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011), goat-anti-rabbit-AF568 (ThermoFisher A11036) ...
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Physiology 2023Quote: ... followed by 1 hour incubation in DMEM/3% bovine serum albumin containing 1 μM AlexaFluor 647 conjugated α-bungarotoxin (Life Technologies) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Cell Biology 2024Quote: ... suspended in arrest-mixture assay buffer (no ATP) with 3% BSA and 1:400 anti-CARMIL1 antibody (ThermoFisher, 27133-1-AP). After incubation for 2 hr at RT ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were trapped on a C18 reversed-phase trap column (2 cm x 75 μm ID; Acclaim PepMap trap column packed with 3 μm beads, Thermo Fisher Scientific) and separated on a 25 cm C18 reversed-phase analytical column (25 cm x 75 μm ID ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... were concentrated and desalted on a trapping pre-column (Acclaim PepMap C18, 75 µm × 2 cm nanoViper, 3 µm, 100 Å, Thermo Scientific), using 0.2% formic acid at a flow rate of 5 µl/min ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide sample (2 μL) was injected onto an Acclaim PepMap 1000 trap column (75 μm × 2 cm, nanoViper C18 3 μm, 100Å, Thermo Fisher Scientific) which was heated to 40 °C in a chamber which was connected to a C18 reverse-phase Aurora UHPLC Emitter Column with nano Zero & Captive Spray Insert (75 μm × 25 cm ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS (3 x 10 min) and incubated 2 hours with the appropriate secondary antibodies (Invitrogen, CA, USA) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Cells were electroporated with 2 μg of donor construct and 3 μg of px330-Cas9 plasmid using a Neon Transfection System (Thermo Fisher Scientific) and the following settings ...
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bioRxiv - Biophysics 2020Quote: ... We grew HL-60 cells to approximately 1 × 106 cells/ml and passaged the cells every 2-3 days in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with L-glutamine and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Biochemistry 2022Quote: The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480 for replicate 2, and Q-Exactive HF for replicate 3, Thermo Fisher Scientific). Peptides were sampled on a precolumn (300 μm × 5 mm PepMap C18 ...
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bioRxiv - Immunology 2022Quote: ... Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 μm, nanoViper, 75 μm x 2 cm, Thermo Fisher Scientific) with 5 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... spectra were recorded on a Thermo-Finnigan Surveyor equipped with a Satisfaction RP18AB C18 3 μm (15×2 mm) (Cluseau) and coupled to an ESI LCQ Advantage (Thermo Fisher Scientific). The running mixture was subjected to filtering on a Microcon filter unit of 10 kDa (15 min ...
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bioRxiv - Microbiology 2021Quote: ... using an Acclaim PepMap100 pre-column (C18, 3 μm-100 Å) and a PepMap RSLC (C18, 2 μm, 50 μm-15 cm) both from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... peptides were loaded onto a trap column (PepMap C18 trap column 75 µm × 2 cm, 3 µm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μL/min for 3 min before switching the pre-column in line with the analytical column (PepMap C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Systems Biology 2019Quote: ... equipped with a reverse phase trap column (Acclaim PepMap 100, 75 µm x 2 cm, 3 µm, 100 Å; Thermo Fisher Scientific) and 50 cm analytical column (EASY-Spray ...
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bioRxiv - Cancer Biology 2020Quote: ... LC separation was executed with a trap column (C18, 75 μm*2 cm, 3 μm) (cat No. 164946, Thermo Fisher Scientific, Germany) and an analytical C18 column (C18 ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were loaded onto a trap column (Dionex-C18 trap column 75 μm × 2 cm, 3 μm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μl/min before switching the pre-column in line with the analytical column (Dionex-C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific), and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos ...
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bioRxiv - Cell Biology 2022Quote: ... a Thermo Scientific EASY-nLC 1000 liquid chromatography system coupled with an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column in line with an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... For KV2.2 channels, NDS was substituted for normal goat serum (NGS, 3%) and Alexa Fluor® 555 goat anti-mouse IgG1 (Thermo Fisher Scientific Cat# A-21127 ...
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bioRxiv - Immunology 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. × 2 cm, Thermo Fisher Scientific) equilibrated with 0.05 % TFA in wather ...
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bioRxiv - Neuroscience 2022Quote: ... YFP-positive cells from 2-3 mice of each genotype were collected after viability screening with propidium iodide staining (ThermoFisher, Cat. # P3566) on a Beckman Coulter MoFlo platform in the Flow Cytometry and Cell Sorting Facility located in the Ottawa Hospital Sprott Center (Ottawa ...
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bioRxiv - Neuroscience 2022Quote: ... The peptides were trapped on an guard column (Acclaim PepMap 100, 75 µm x 2 cm, nano viper, C18, 3 µm, 100 Å, Thermo Fisher) using solvent A (0.1% Formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were loaded onto Acclaim PepMap 100 C18 column (75 µm x 2 cm, 3 µm particle size and 100 Å pore size; #164946, ThermoFisher Scientific) and separated on nano-capillary C18 column ...
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bioRxiv - Biochemistry 2024Quote: ... Injected sample fractions were preconcentrated and further desalted online using a PepMap C18 nano trap column (2 cm X 75 μm; particle size, 3 μm; pore size, 100 Å; Thermo Fisher) with a flow rate of 3.5 μL/min for 6 min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. x 2 cm, Thermo Fisher Scientific) equilibrated with 0.05% TFA in water ...
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bioRxiv - Cell Biology 2024Quote: ... water (5:3:2) and 3 µL was injected into a Ultimate 3000 UHPLC system coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific). Separation was done on an Accucore C18 column (150 x 2.1 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptide lysates were initially trapped on a C18-reverse phase trapping column (Acclaim PepMapTM 100, 75 μm × 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), and then separated on a C18-reverse phase analytical column (Double nanoViper™ PepMap™ Neo ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... as separation column and an Acclaim PepMap 100 C18 column (75 µm inner diameter, 2 cm length, 3 µm particle size from Thermo Fisher Scientific) as trap column was used ...
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bioRxiv - Neuroscience 2023Quote: ... One microgram of tryptic peptides (in triplicate) were loaded on a C18 Acclaim PepMap100 trap-column (75 µm ID x 2 cm, 3 µm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 µL/minute with 2% ACN ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat. No. M6494, ThermoFisher Scientific, Waltham, MA, USA) to differentiate the range of toxic and nontoxic palmitic acid concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... then washed 3X with PBS before mounting on 2” x 3” microscope slides with Prolong Glass hardset mounting media (Life Technologies, #P36984). Slides were allowed to dry overnight at room temperature before imaging.
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bioRxiv - Cancer Biology 2023Quote: ... OCI-AML-2 and OCI-AML-3 cells were electroporated with sgRNA/Cas9 complex using the Neon transfection system (Thermo Fisher Scientific) under the condition of 1350 V ...