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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... by using a Fluorostar Omega plate fluorescence plate reader (540 nm excitation, 590 nm emission) after incubation with 10% Presto Blue (Life Technologies) for 1h at 37C ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells were pre-cultured to exponential phase in 180 μl of YES in 96-well culture plates at 32°C in the VarioSkan Flash plate reader (Thermo Scientific). 5 μl of the preculture were used to inoculate 175 μl of fresh YES in 96-well culture plates and incubated for ∼48 hours in the VarioSkan Flash plate reader at 32°C with background shaking (1080 spm ...
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bioRxiv - Systems Biology 2022Quote: ... filtered by positive pressure and the flowthrough was collected in a glass coated microtiter plate (WebSeal Plate+ 96-Well Glass-Coated, 300 - Fisher Scientific); 3 ...
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bioRxiv - Neuroscience 2023Quote: ... for ten minutes in a 96-well plate (protected from light) and the fluorescence was read at 520 nm using a Varioskan Flash plate reader (ThermoFisher, UK).
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Plant Biology 2024Quote: ... 3 μl of the dilution series and cocktail mix were dispensed into duplicate wells on a 384-well ABI optical plate and the plate was loaded on the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). The FAM-Non Fluorescent Quencher detector and Rox passive reference were selected ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then transfected with 100 ng/well (96-well plates) or 500 ng/well (24-well plates) indicated plasmids using Liopfectamine 3000 (Invitrogen, USA) for 24 h or as specified ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Physiology 2023Quote: ... From the tubes 300μl of all samples were transferred to ELISA plate in triplicates and the absorbance was read at 520nm using ELISA plate reader (ThermoFisher™). Optical Density (OD ...
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM) and diluted in DMSO transferred to wells of PCR Plates (Micro Amp Fast 96-well Reaction Plates, Applied Biosystems) to ensure matching final DMSO concentrations.
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bioRxiv - Cancer Biology 2023Quote: ... siRNAs were transfected at 10ng/well in 384 well plates and 40ng/well in 96 well plates using Lipofectamine RNAiMAX (Invitrogen, #13778075). Imaging and drug incubation experiments were conducted in 384 well plates ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial growth was monitored by measuring OD600 and for growth experiments performed in airtight sealed microtiter plates (sealing tape for 96-well plates, Thermo Scientific), a Power Wave XS microplate reader (BioTek Agilent ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μL aliquots of infected cells were deposited in a white 96-well plate (F96 MicroWell™ Plates, non-treated form Nunc) and the luminescent signal was measured with the help of the plate reader.
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bioRxiv - Biophysics 2023Quote: ... 8 µl of ATP-ATPase reaction mix were transferred from row A on master mix plate to respective row (B to H) on reaction stop plate using a MatrixTM Multichannel Pipette (Thermo Fisher). Additionally ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Microbiology 2023Quote: ... pre-cultures were prepared by inoculating single colonies from a LB agar plate in 200 μl of liquid LB in a 96-well plate (Nunc, Thermo Scientific). After 22 h of growth at 37°C with 250 rpm shaking ...
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bioRxiv - Microbiology 2023Quote: ... gondii growth were determined in HFF cell cultures using 96-well black plates (Optical-Bottom Plates with Polymer Base, ThermoFisher Scientific). Sulfadiazine (positive control ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Microbiology 2024Quote: ... and GFP fluorescence (excitation 485 nm, emission 520 nm) were measured in microtiter plates using a Varioskan Lux plate reader (Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... monolayer permeability was assessed by transferring the transwell plates in a new receiver plate containing 200 µl Hank’s balanced salt sodium (HBSS) (Gibco 14025-092). To determine permeability of the monolayers (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: The proteolytic activity of activated globupain and its substitution variants was assessed using the casein gelzan™ CM plate assay and the EnzChek™ Protease Assay Kit (Thermo Fisher Scientific, MA, USA), respectively ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS and resuspended in 1 mL FACS buffer with 1 μL of LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen). HeLa-ACE2 cells alone and with SARS-CoV-2 Spike only were used as background and positive controls ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse-transcribed at 50°C for 1 h ourwith SuperScript III and 1 mM of each dNTP (SuperScript III cDNA synthesis kit, Invitrogen) followed by 18-cycle PCR amplification.
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bioRxiv - Bioengineering 2019Quote: ... the blot was then developed in 1:1 (v/v) dilution of SuperSignal™ West Femto Trial Kit (ThermoFisher Scientific) to achieve the desired signal intensity in the Amersham Hypercassette™ Autoradiography Cassettes.
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bioRxiv - Bioengineering 2019Quote: ... centrifuged cells were resuspended in PBS and stained with AlexaFluor 488-conjugated annexin V and 1 µg mL-1 propidium iodide (PI) as per kit manufacturer recommendations (V13241, ThermoFisher). Cells were analyzed on a BD LSR Fortessa X20 (UC Berkeley Cancer Research Laboratory Flow Cytometry Facility) ...
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bioRxiv - Neuroscience 2021Quote: ... The cell lysate was mixed 1:1 with 70% ethanol and RNA was extracted according to the guidelines of the PicoPure RNA Isolation Kit (ThermoFisher). RNA was dissolved in 11 μl nuclease-free H2O ...
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bioRxiv - Neuroscience 2021Quote: ... Capped cRNAs were synthesized from TALEN pairs mixed 1:1 using the mMESSAGE mMACHINE T3 Transcription kit (Life Technologies, Canada) and purified using the Oligotex mRNA Mini Kit (Qiagen Inc. ...
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The section of the membrane with separated 300 - 95 kDa proteins was incubated with primary antibodies against C-terminus of Pol2 described in the previous section at 1:500 – 1:1,000 dilution in diluent provided in SuperSignal Western Blot Enhancer Kit (Thermo Fisher). Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030 ...
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bioRxiv - Microbiology 2022Quote: ... The culture was pelleted at 4000 rpm for 2 min and resuspended in fresh LB containing 1% of the “DQ starch substrate stock solution” (1 mg/ml, EnzChek Ultra Amylase Assay Kit, Invitrogen Detection Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... signal amplification was performed with Alexa Fluor 555 Tyramide conjugate (1:50) in 1× reaction buffer supplied with Alexa Flour 555 Tyramide SuperBoost kit (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Goat Anti-Rabbit IgG secondary antibody poly HRP (1:1, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots were further diluted 1:10 with the salt solution before staining 1:1 with a mixture of SYTO9 and propidium iodide (LIVE/DEAD BacLight Bacterial Viability Kit, Invitrogen) and incubating in the dark at room temperature for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated in round-bottomed 96-well plates (1×106 cell per well) in ʟ-arginine-free RPMI-medium (SILAC RPMI-medium, Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated in round-bottomed 96-well plates (1×106 cell per well) in ʟ-arginine-free RPMI-medium (SILAC RPMI-medium, Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Immunology 2019Quote: ... SV40-immortalized D8-KODO MEFs were infected with GFP-expressing MCMV at an MOI of 1 for 24 hours and subsequently released from plates with Versene (Thermo Fisher, Waltham, MA) prior to antibody staining and flow cytometric analysis.
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bioRxiv - Cell Biology 2020Quote: 1.5 × 104 cells/cm2 were transfected in a gelatin-coated 12-well plate in N2B27-2i using DharmaFECT 1 (Fisher Scientific, T-2001). For RNAi assays esiRNA for Eif4a2 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Cell Biology 2020Quote: Cells from one-day-old culture - at the mid-exponential growth phase - at an initial cell density of 1×105 cells / ml were seeded in 12-well plates (Nunc/DD Biolab, #55428) with 1 ml marine broth and incubated at 23 ºC ...
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bioRxiv - Plant Biology 2020Quote: Four to six-days old seedlings were incubated in 24-well cell-culture plates for 1 hour in 50 μM BFA (Invitrogen, Thermo Fisher Scientific) containing liquid growth medium (0.5x MS medium ...
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bioRxiv - Immunology 2020Quote: ... monocytes were allowed to adhere to culture plates (Costar) for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The cells were then washed with warm phosphate buffered saline (PBS ...