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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... to approximately 5 µl and 4 µl were transformed into 100 µl of ElectroMax DH5α-E Competent Cells (Cat# 11319019, Thermo Fisher Scientific). After 30 min of recovery at 37°C in 4 ml of SOC medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Triton X-100 and 5% goat serum and incubated overnight at 4°C and DNA was labeled using Hoechst (Thermo Fisher, H3570). Images were subsequently captured with confocal microscope ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 150V for 120 min and transferred to 0.45 μm PVDF membranes (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by centrifugation at (21,000 g, 5 min, 4 °C) and endogenous metabolites were removed with Zeba Spin Desalting Columns (ThermoFisher, cat. no 89882). The lysate was then split into sample replicates for PISA (four replicates for each treatment) ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Cell Biology 2024Quote: ... the grids were blotted for 3.5 s at a humidity of 100% and 4°C and plunge-frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Dd2-Polδ mixed-stage parasite cultures at 5% parasitaemia and 4% hematocrit were dispensed into each well of a six-well flat-bottom plate (Thermo Scientific, Australia). Five cultures were treated with 10x the growth EC50 of OGHL250 (9.1 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were post-fixed in 4% PFA for 5 min and mounted with ProLong Glass Antifade Mountant (Thermo Fisher Scientific Cat# P36980). Confocal scanning was performed at RT on either a Nikon C2 confocal microscope equipped with photomultiplier detectors using 40x Plan Fluor ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was then centrifuged at 300 × g for 5 min at 4 °C and cells resuspended in 100 µL of stain buffer (BD Biosciences, supplemented with 5 mM EDTA (Thermo Fisher Scientific)) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were centrifuged at 400 g for 5 min at 4 °C with no brake and resuspended in staining buffer (DPBS (without Ca2+ and Mg2+) (Gibco, Life Technologies) containing 0.1 % low endotoxin BSA (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were boiled at 95°C for 5 min and 10 µl of each sample was separated by 4-12% Bis-Tris SDS-PAGE (Thermo Scientific NW04122BOX) in 1×MES buffer (Thermo Scientific B0002 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... 5 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific; NP0432) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed five times with RIP buffer for 5 min at 4°C and RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Reduced samples (by addition of 2-mercaptoethanol) were heated at 95°C for 5 minutes then loaded onto a 4-12% tris-glycine gradient gel (Thermo Fisher, XP04122BOX) and ran at 125 volts for 1-1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... were boiled for 5 min and subjected to SDS-PAGE using NuPAGE™ 4-12% Bis-Tris Gels (Invitrogen; Thermo Fisher Scientific) at 120V for 80 min and transferred to 0.45 µm PVDF membranes (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of plasmid was transfected into 5*105 cells using a Neon nucleofector (ThermoFisher, 4×10 ms pulses at 1230 V) and given 48 hours to recover ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then boiled at 95°C for 5 min and electrophoresed using a NuPAGE Bis-Tris Gel (4-12%) with NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Genetics 2024Quote: ... Samples were denatured at 99°C for 5 minutes and separated on a 4-20% tris-glycine protein gel (Thermo Fisher Scientific) at 150 V for 75 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were boiled in SDS-PAGE sample buffer for 5 min and separated by electrophoresis on NuPAGE 4–12% Bis-Tris SDS gels (Thermo Fisher Scientific) using NuPAGE MES SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The beads were resuspended in 20 µl of sample buffer (5 µl of 4× Bolt LDS sample buffer (B0007, Thermo Fisher Scientific), 14 µl of PNK buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then heated at 95°C for 5 minutes before loading on to the Bolt 4-12% BisTris Gel (Thermo Fisher Scientific). After electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination (17 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Biochemistry 2024Quote: ... Four μl of sample with an OD260 of 7.5 were applied to the grids and vitrified at 4 °C and 100 % humidity using a Vitrobot Mk IV (Thermo Fisher Scientific) (blot 6s ...
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bioRxiv - Immunology 2024Quote: ... Cytocentrifugation was performed with 200 μL of lavage fluid at 300 g for 5 minutes using a Cytospin 4 (Thermo Fisher Scientific). BALF samples were imaged in an automated BZX-810 microscope at 40x ...
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bioRxiv - Bioengineering 2024Quote: ... The cell suspension was then filtered through a 70um cell strainer and pelleted at 400 xG for 5 min at 4°C followed by RBC lysis using ACK lysis buffer (Gibco A10492-01) for 5 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were immunoblotted at 4°C overnight with the following antibodies in 5% BSA/1x TBST: MKP1/DUSP1 (1:1000, Invitrogen #MA5-32480), DUSP3 (1:2000 ...
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bioRxiv - Genomics 2024Quote: ... centrifuged at 350 x g for 5 min at 4 ºC and pellets resuspended in appropriate volume of PBS (Thermo Fisher Scientific) supplemented with 0.05% BSA (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2024Quote: Purified MglAGDP and MglB were labeled overnight at 4°C under gentle agitation in the presence of a 5-fold excess of Alexa Fluor™ 488 C5-maleimide (ThermoFisher scientific) or Janelia Fluor® 646 Maleimide (TOCRIS ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... Samples were eluted with Laemmli buffer and denatured at 95°C for 5 min before loading to 8% or 4-20% Tris-Glycine gels (Thermo Fisher Scientific). After transferring ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mL of each culture was collected and cells were harvested by centrifugation (6,000 rcf; 5 min; 4 ℃) Pelleted cells were suspended in B-PER (ThermoFisher, Cat. No.: 90084), supplemented with 1 mM TCEP ...
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bioRxiv - Developmental Biology 2024Quote: ... Around 1% of the sample was set aside as input and the remaining supernatant was incubated overnight at 4°C with 5 µg of anti-CtBP2 antibody (Invitrogen, PA5-86439), anti-HDAC1 antibody (Diagenode ...
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bioRxiv - Developmental Biology 2024Quote: ... aegypti embryos at specified developmental stages (0-1h, 4-5h, 5-6h, 23-24h, 47-48h, and 71-72h) using Trizol® reagent (Ambion by Life Technologies), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated for 30 min at 4°C with 5 μM of MitoSOX and 100 μM of MitoTracker (Thermo Fisher Scientific) to assess mitochondrial superoxide production and mitochondrial mass ...
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bioRxiv - Developmental Biology 2023Quote: ... Pax9 was cloned from 24 hpf cDNA using the primers pax9F 5’-TCTAGAATGGAGCCAGCCTTT-3’ and pax9R 5’-ATGGATCCTCATAGAGCTGAAGCCACCAG-3’ (Supplementary Table 6) and cloned by TOPO-TA to the pCRII vector (Invitrogen) to create pCRII pax9 ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Genomics 2021Quote: ... consisting on regular DMEM supplemented with 1x N-2 supplement (Thermo Fisher Scientific #17502048), 1x B27 supplement (Thermo Fisher Scientific #12587010 ...
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bioRxiv - Bioengineering 2022Quote: ... [2-(N-morpholino)ethanesulfonic acid] (MES) buffered saline pack was purchased from Thermo Scientific. Chemicals sensitive to moisture ...
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bioRxiv - Immunology 2024Quote: ... washed with CSB and left O/N in 2% formaldehyde (PFA) (Thermo Fisher Scientific) at 4°C.