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Citations for Thermo Fisher :
551 - 600 of 7736 citations for Recombinant Human PTGS2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.
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bioRxiv - Biochemistry 2021Quote: Modified pBAD/His B vector (Invitrogen) containing native dnrK and tamK31,39 were used as templates for chimeragenesis ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and Hi-5 cells cells (Invitrogen), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10% HI-FBS (Thermo Fisher, 16140071) prior to adding indicated concentration of human IFN-alpha 2b (pbl Assay Science ...
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bioRxiv - Immunology 2024Quote: ... in Hi-Di formamide (Thermo Fisher) on an ABI Gene Analyzer 3500XL and analyzed using GlycanAssure version 1.0 (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... and 10% HI-FBS (Gibco 16250078). Cells were detached using 5mL 0.25% Trypsin-EDTA (Gibco 25300054) ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant PAK6 (ThermoFisher, #PR5307A) was diluted in blocking buffer and incubated with the arrays at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pDisplay encoding myc-tagged GluA1-GluA4-ATD or pCAGGS encoding FLAG-tagged Nrx or GFP with Lipofectamin 2000 (Invitrogen). On the following day ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Cancer Biology 2019Quote: ... bait GST-tagged EIF4E or 6xHis-tagged CELF1 proteins (1 μM) were initially bound to glutathione-agarose beads (ThermoFisher Scientific) or Ni2+ beads (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: GST-tagged full-length SYK and VenusFlag-tagged full-length FCER1G were generated using Gateway LR Clonase (Invitrogen, 11791-100) for mammalian cell expression ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa555 or Alexa 594 tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Cell Biology 2019Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes.
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bioRxiv - Neuroscience 2020Quote: ... fluorescently-tagged polystyrene microspheres (10 micron, Invitrogen F8836) were bilaterally injected into the anterior chambers of Opn4Cre;Ai32 mice at ~6-8 weeks of age (Sappington et al. ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescence-tagged secondary antibodies were purchased from Thermofisher and used at 1:500 dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... horseradish peroxidase-tagged second antibody (Thermo Fisher Scientific) was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL ...
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bioRxiv - Microbiology 2021Quote: ... and Alexa488-tagged phalloidin were obtained from Invitrogen and were used at 1:1000 dilution.
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bioRxiv - Molecular Biology 2020Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes. VOA ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa555 or Alexa 594-tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Developmental Biology 2022Quote: ... An appropriate Alexa Fluor-tagged secondary antibody (Invitrogen) was matched to the primary and incubated for 1-2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CXCL1 and CXCL2 (FAM tagged; Thermo Fisher Scientific) on a Biorad CFX Connect™ machine ...
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bioRxiv - Cell Biology 2024Quote: ... Amp-3 and HRP-tagged probe (ThermoFisher Inc) for 30 ...