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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... following which protein-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) following manufacturer’s instructions ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylated probes were hybridized to GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... we delivered recombinant Streptococcus pyogenes Cas9 protein (TrueCut™ Cas9 Protein v2; Thermo Fisher Scientific) together with the crRNA and tracrRNA into in vitro fertilized eggs of C57BL/6J mice according to the institutional procedure of Kyoto university (Ishibashi et al. ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Biophysics 2023Quote: Recombinant baculovirus was generated by transfecting Sf9 cells with bacmid using Cellfectin II (ThermoFisher). Baculoviruses were harvested from Sf9 cell media by filtering through a 0.22 µm filter and amplified three times before using for cell transduction ...
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bioRxiv - Biochemistry 2021Quote: ... The Strep (II) elution fractions were further purified via ion exchange chromatography (Poros HQ 50 μm, Life Technologies) and subjected to size-exclusion chromatography in a buffer containing 50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... 0.05mM 2-mercaptoethanol and 1% Pen/Strep) and enumerated using the automated cell counter Countess II (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Microbiology 2022Quote: ... human recombinant epidermal growth factor (0.2 ng/mL, all from Gibco, Schwerte, Germany), isoproterenol (1 nM ...
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bioRxiv - Bioengineering 2022Quote: ... or 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700) for serum-free cells ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µg/mL human recombinant insulin (ThermoFisher Scientific #12585014; stock = 4 mg/mL), 1:100 Penicillin-Streptomycin (ThermoFisher Scientific #15140122) ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated proteins were pulled down with Pierce High Capacity Streptavidin Agarose (20357; ThermoFisher) and were separated by SDS-PAGE and visualized using a SilverXpress silver stain kit (LC6100 ...
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bioRxiv - Neuroscience 2019Quote: ... Immunoprecipitation (IP) of transcripts was achieved via biotinylated-protein L (ThermoFisher Scientific 29997)-coated streptavidin magnetic beads (ThermoFisher Scientific 65602 ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified using 260μl MyOne Streptavidin T1 beads (Novex Life Technologies) per 5mg total protein as described previously (22) ...
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bioRxiv - Microbiology 2020Quote: ... S1 proteins were pre-biotinylated using EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific). Following 20 minutes of pre-hydration of SA biosensors and 1 minute of sensor check ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated protein was purified using a Zeba spin desalting column (Thermo Fisher Scientific). ACE2-Fc was then diluted to 20-83.3 mg/mL in PBS and loaded onto streptavidin biosensors (Forte Bio) ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated using a Fluoreporter Mini-biotin XX protein labeling kit (Life Technologies) and detected using streptavidin-Qdot 585 ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated-CoV proteins were captured on streptavidin coated 96-well plates (Thermo Scientific) by incubating with 100 µL of 2 µg/mL protein solution in TBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... L-AHA labelled proteins were biotinylated using PEG4 carboxamide-Propargyl Biotin (ThermoFisher, B10185) and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured using Pierce Streptavidin Magnetic Beads (Thermo Fisher Scientific, 88817) at 4°C on a rotator for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 594 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were tetramerized by adding APC-conjugated streptavidin (Thermo Fisher Scientific) at an allergen ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotinylated proteins were detected with NeutrAvidin DyLight 800 (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Microbiology 2021Quote: ... 5% A+ human serum and 5% AlbuMAX II (Life Technologies). Gametocyte media was changed daily without the addition of fresh erythrocytes for 14 days following induction ...
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bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Microbiology 2021Quote: ... Purification of His-tagged Cg1978 was performed by applying the supernatant to an equilibrated 2 ml Ni-NTA column (Invitrogen, Kalifornien, USA). After washing with 30 ml TNI20 buffer ...
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bioRxiv - Immunology 2022Quote: ... Expi293F™ cells were transiently transfected with His-tagged gB ectodomain plasmids for five days and later harvested with Nickel-NTA resin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant protein was transiently expressed in Expi293™ (ThermoFisher Scientific, UK) and purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen) and purified by nickel affinity chromatography ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μL/sample of recombinant Protein-G-Sepharose-4B beads (ThermoFisher) was washed thrice with IP buffer and added to each sample for equilibration on a rotator for 4h ...