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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... We immunoprecipitated GFP-tagged RBBP4 and RBBP7 proteins with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... TAP- or FTpA-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific) for 2.5 h at 20°C ...
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bioRxiv - Biochemistry 2024Quote: ... to assess FLAG-tagged protein expression and immunoprecipitation by Immunoblot and silver stain (ThermoFisher Scientific) respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-tagged protein was purified by affinity column chromatography using Ni-IMAC resin (Thermo Fisher) followed by size exclusion chromatography using a Superdex 200 column (Sigma Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Verification of a successful GST-tagged AP2 protein purification by GST-affinity purification (Thermo Scientific Pierce Glutathione Superflow Agarose beads ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10ng/ml recombinant human basic Fibroblast Growth Factor (bFGF) and 1x B27 supplement (Life Technologies). Cells were then counted and passed through a 25G needle to obtain a single cell suspension ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human TNF-α (PHC3011) and antibody against OAS3 (PA5-31090) was from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific AG), 1% penicillin and streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Cancer Biology 2024Quote: ... + 500 U/mL Gibco™ recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Molecular Biology 2020Quote: ... The isolated protein complex was eluted by incubating the beads overnight at 4°C with 200 units of TEV protease (Thermo Fisher Scientific) in 500 µl of TEV150 buffer ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies and recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... we used PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Biochemistry 2022Quote: Recombinant proteins were labeled with Alexa Fluor(tm) 647 NHS ester (Thermo Fisher) as described previously18 ...
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bioRxiv - Biochemistry 2022Quote: ... the recombinant protein mixtures were combined with 12.5 μM diFMUP (Life Technologies, E12020), added to 384-well plates ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... the recombinant proteins were dialyzed using Slide-A-Lyzer™ cassettes (Thermo Fisher) into cold coupling buffer containing 100mM NaHCO3 and 500mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Spike protein was labelled with Alexa Fluor™ 546 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Pathology 2022Quote: ... and incubated with PierceTM Recombinant Protein L Biotinylated (60µL per kidney, ThermoFisher #29997) in a tube rotator (25min. ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant N protein was purified using Ni2+ resin (Thermo Fisher Scientific, USA). An indirect ELISA assay was conducted with the recombinant N protein as the coating antigen (3 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified recombinant Cas9 proteins (250 ng/ml: Thermo Fisher Scientific, MA, USA). The gRNA sequences are as follows:
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bioRxiv - Cell Biology 2019Quote: Mouse TGF-β 1 recombinant protein was obtained from Affymetrix (#14-8342-62) and used at the concentration of 2 ng/ml unless stated otherwise ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... The samples were mixed with recombinant protein G-Sepharose 4B beads (Thermo Fisher) and 1 µl anti-Flag antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...