Labshake search
Citations for Thermo Fisher :
551 - 600 of 7789 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... anti-rat-HRP (1:10000, #31470 Fisher Scientific) and anti-goat-HRP (1:10000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and goat anti-rat AF647 (Invitrogen; A-21247), then washed 3 times with PBS and mounted using Fluoromount ...
-
bioRxiv - Plant Biology 2019Quote: ... in frame with a downstream GFP reporter gene in the pB7FWG,0 plasmid, or in the pB7WG plasmid respectively (Ghent plasmids collection, http://bccm.belspo.be/index.php) via Gateway technology (Invitrogen). As the S2Lb fragment was cloned without STOP codon ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Fisher) per manufacturer protocols ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids were isolated using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and Sanger sequenced with the universal primers M13Forward and M13Reverse.
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids were isolated using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and quantified by spectrophotometric analysis (Biophotometer ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid was purified using Purelink-Midi plasmid purification kit (Invitrogen). Plasmid transfection was done using Fugene HD (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... plasmids extracted using a GeneJet Plasmid Purification Kit (Thermo Scientific), and constructs sequence-verified using Sanger sequencing (Genewiz).
-
bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were isolated (GeneJET Plasmid Miniprep kit, Thermo Fisher Scientific) and confirmed by restriction enzyme digestion and/or Sanger sequencing (Eurofins ...
-
bioRxiv - Plant Biology 2023Quote: ... The plasmids were extracted using Plasmid DNA Miniprep Kit (ThermoFisher), and the sequences were confirmed by sequencing of the ligation sites (Macrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... followed by plasmid isolation (GeneJET Plasmid Miniprep System; Thermo Fisher). Isolated plasmids were test digested and sequenced.
-
bioRxiv - Molecular Biology 2021Quote: ... Cells with stable integration of the shRNA construct were determined by a selection of puromycin-resistant colonies (0.5 μg/ml puromycin; Invitrogen). The efficacy of TRMT2A silencing effect was determined by qPCR and Western blot ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
-
The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... and the annealed shRNA oligos were inserted into the digested vector using the T4 DNA ligase (ThermoFisher Scientific, #EL0014). The lentiviral particles were packaged via co-transfection of FH1-tUTG vector inserted with shRNA oligos (44 μg) ...
-
bioRxiv - Cell Biology 2022Quote: ... The shRNA target sequence was designed for protein knockdown using the BLOCK-iT RNAi Designer tool (Thermo Fisher Scientific). A cassette containing the pre-shRNA sequence was inserted into pBAsi-mU6 (Takara Bio) ...
-
bioRxiv - Molecular Biology 2022Quote: ... shRNA sequences targeting the murine Uhmk1 gene were designed using the BLOCK-IT™ RNAi Designer tool (Invitrogen™), cloned into the MSCV-U3-H1-Stuffer entry vector digested with BglII and HindIII and subcloned into the retroviral vector pMSCV-puromycin-IRES-EGFP siRNA digested with the NotI and ScaI [55] ...
-
bioRxiv - Cell Biology 2023Quote: Two sets of USP3 shRNAs were designed against its target sequence and cloned independently into pSilencer2.1-U6 hygro vector (Ambion). Scrambled shRNA with limited homology to any known sequences was cloned into the same vector and could serve as the negative control ...
-
bioRxiv - Genetics 2023Quote: ... RKO and HT-1080 shRNA+ cells from the competition proliferative assay experiment were selected with Geneticin/G418 Sulfate (Gibco) for 7 days and checked for GFP expression ...
-
bioRxiv - Immunology 2023Quote: ... lentiviruses for individual shRNAs were produced in HEK293T cells plated in six-well plates in antibiotic-free DMEM (Gibco, supplemented with glutamine and 10 %FBS) ...
-
bioRxiv - Neuroscience 2024Quote: ... The ssDNA primers to generate the shRNAs were either obtained using the Block-it RNAi web tool (Thermo Scientific) or designed by hand and were as follows:
-
bioRxiv - Immunology 2020Quote: ... pCDNA3.3 plasmids (ThermoFisher) encoding the WT (NC_045512 ...
-
bioRxiv - Bioengineering 2019Quote: ... and stained with IL2-XE114-TNFmut (final concentration 10μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) followed by staining with anti-rat AlexaFluor488 (Invitrogen A21208). IL2-KSF-TNFmut (specific for an irrelevant antigen ...
-
bioRxiv - Cell Biology 2019Quote: ... 3 rat anti-Rab1 and 3 rat anti-Rab11 (1:2000 concentrated supernatant) as primary antibodies and HRP-conjugated anti-rat IgG antibodies (1:20000, Life Technologies) as secondary antibodies ...
-
Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were stained with unconjugated lineage rat antibodies (CD3, CD4, CD5, CD8, B220, Gr1, Mac1, and Ter119) followed by goat-α-rat PE-Cy5 (Invitrogen). Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... M-100126-01-0005), rat Esrrg (Dharmacon, M-100212-01-0005), rat E2f1 (Dharmacon, M-102754-00-0005) or scramble control siRNA (ThermoFisher, 4390843), using the Viromer BLUE Transfection Reagent (Lipocalyx ...
-
bioRxiv - Neuroscience 2022Quote: ... The expression of Kir2.1EGFP was assessed with anti-GFP antibody (rat monoclonal Ab from Nacalai, Japan, GF090R; the secondary antibody, 488-anti Rat IgG from Invitrogen, A11006) (Mizuno et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
-
bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
-
bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plasmid DNA was isolated using a Plasmid Midiprep Kit (Thermo Fisher) and stored in TE buffer (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2020Quote: ... Plasmids were purified using a GeneJet Plasmid Miniprep kit (Thermo Scientific). 50μg/mL Phleomycin (Invivogen ...
-
bioRxiv - Neuroscience 2020Quote: ... The final plasmid and the Flp recombinase vector (pOG44 plasmid, Invitrogen) were packaged with Lipofectamine2000 (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... plasmid DNA-TurboFect and plasmid DNA-Lipofectamine 2000 (ThermoFisher Scientific, USA) complexes were prepared following the manufacturer’s protocol in serum-free OptiMEM (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Plasmids transfections were performed with indicated plasmids using Lipofectamine 2000 (ThermoFisher) or genejuice reagent (EMD Millipore ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were extracted using the PureLink Quick Plasmid Miniprep kit (Invitrogen) before transfer to M ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified using a GeneJet Plasmid Miniprep kit (Thermo Scientific). 50µg/mL Phleomycin (Invivogen ...
-
bioRxiv - Cell Biology 2022Quote: ... The plasmid was purified (Purelink HiPure Plasmid Maxiprep Kit, Invitrogen, CA) from transformed Stellar Competent Cells (Takara Bio Inc ...
-
bioRxiv - Microbiology 2019Quote: ... Plasmids were purified using the Genjet Plasmid Miniprep MKit (Thermo Scientific). LPC (15:0 ...
-
bioRxiv - Molecular Biology 2019Quote: ... plasmids was recovered with GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and the DNA was sequenced with M13 common sequencing primers ...
-
bioRxiv - Physiology 2020Quote: ... Plasmids were isolated using PureLink HiPure Plasmid Midiprep Kit (Thermo Scientific) and sequenced by GATC Biotech AB (Köln ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmids were isolated using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
-
bioRxiv - Physiology 2021Quote: ... Plasmid DNA was isolated (PureLink Quick Plasmid Miniprep kit; Invitrogen K210010) according to manufacturer specifications and inserts were sequenced to confirm their identity and uploaded to NCBI (MW962257).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The plasmid was purified (Purelink HiPure Plasmid Maxiprep Kit, Invitrogen, CA) from transformed Stellar Competent Cells (Takara Bio Inc ...
-
bioRxiv - Molecular Biology 2022Quote: ... sgRNA plasmids and donor plasmids were transfected with Lipo3000 Reagents (Invitrogen) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
-
bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
-
bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).