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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Catalog# CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: iPSCs colonies were harvested with Accutase® and dissociated into single cells using the complete human embryonic stem cell (hESC) medium consisting of DMEM/F12 (11320033, ThermoFisher), 5x knockout serum replacement (10828010 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... human ileocecal cell line from 25 to 35 passages were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 4mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 (anti-human CD3 for NK cell experiments) according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2023Quote: BHK-21 and CFBE41o- human CF bronchial epithelial cell lines were grown in DMEM/F-12 (5% fetal bovine serum; Invitrogen) and MEM (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... MSC-EV preparation vesicular structure and canonical surface marker expression were determined using EV bead-based flow cytometry with human CD81 detection beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... at different timepoints as the percentage of hCD20pos cells (anti-human CD20 FITC, clone 2H7) compared to murine CD45pos cells (anti-mouse CD45 PE, clone 30F11, ThermoFisher). When indicated ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: African green monkey kidney cells (Vero) and Human Embryonic Kidney 293T (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Human A549 or A549ACE2 cells were cultured in a Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco catalog no. 11875) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Biophysics 2024Quote: The immortalized human keratinocytes (HaCaT cells) were cultured in low calcium media made from no-calcium DMEM (21068028, ThermoFisher Scientific), 10% FBS and 1% penicillin-streptomycin at 37°C supplied with 5% CO2 to maintain the dividing state.
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bioRxiv - Immunology 2024Quote: ... Freshly purified CD8+ T cells (day 0) were activated by adding Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) at a cell/bead ratio of 1:0.5 for 48-64h and expanded in complete R10 medium [RPMI-1640 medium containing 20 mM HEPES and 2.05 mM L-glutamine (#7388 ...
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bioRxiv - Cell Biology 2024Quote: Previously generated microarray data from microdissected human glomeruli sourced from individuals with kidney disease and healthy donors were used (Affymetrix HGU133Plus2.0 ...
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bioRxiv - Immunology 2024Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Human fetal lung fibroblasts (MRC-5; ATCC, CCL-171) and human uterine cervix cancer cells (HeLa) were cultured in minimum essential medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Microarray assays were performed using Affymetrix GeneChip Rat Gene ST arrays (for McA cells) or GeneChip Human Gene 2.0 ST arrays (for Huh7 cells) (Applied Biosystem/Thermo Fisher). GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G running Affymetrix Gene Command Console ver 3.2 ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with Alexa Fluor 488-conjugated goat anti-human IgG to stain the RBD (Thermo Fisher Scientific; A11013) at 4℃ for 1 hour ...
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bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Microbiology 2024Quote: ... or the type II ME49 strain Δku80/Δhxgprt background (Waldman et al., 2020) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... compound was incubated with cells expressing human S1PR1 and Gα15 in the culture medium consisting of Ham’s F-12 Nutrient Mixture (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The murine macrophage RAW264.7 cell line and Human colon adenocarcinoma Caco-2 cell line was cultured in DMEM (Gibco: 11995065) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Transcriptome analysis was done using the GeneChip Human Transcriptome Array 2.0 for in combination with the GeneChip WT PLUS Reagent kit (Applied Biosystems). 33,3ng total RNA was used as input for the analysis that was done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... ELISAs for total IgM or IgG were also performed in parallel using human uncoated IgM or IgG ELISA kits (Invitrogen), following manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: The human dynein motor domain locked in α-registry was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described (21) ...
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bioRxiv - Biochemistry 2024Quote: Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media (Gibco, Thermo Scientific, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin antibiotics at 37 °C in a 5% CO2 incubator ...