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5801 - 5850 of 10000+ citations for Recombinant Mouse Folh1 His tagged since 2019

• PSMA-bearing extracellular vesicles secreted from prostate cancer convert the microenvironment to a tumor-supporting, pro-angiogenic state

  Camila Maria Longo Machado, et al., bioRxiv - Cancer Biology 2022

  Quote: ... the slices were incubated with a secondary goat-anti-mouse or rabbit Alexa-488 or Alexa 563 antibodies or goat-anti-mouse-HRP (Invitrogen, Life Technologies, Carlsbad, CA) for 2 h followed by DAPI cell nuclei staining or Hematoxylin staining ...
• Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons

  Emma T. Watson, et al., bioRxiv - Neuroscience 2022

  Quote: ... Mouse hippocampal neurons were plated on 18 mm coverslips that were coated in poly-D-lysine and mouse laminin (Thermo Fisher Scientific, 23017015) for 2 hours at 37°C ...
• Nucleolar-based Dux repression is essential for 2-cell stage exit

  Sheila Q. Xie, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
• Deposition chamber technology as building blocks for a standardized brain-on-chip framework

  B. G. C. Maisonneuve, et al., bioRxiv - Bioengineering 2021

  Quote: ... and Tau (mouse monoclonal, Thermo Fisher, AHB0042, dilution 1:1000; secondary antibody: goat anti-mouse Alexa Fluor 647, Life Technologies, dilution 1:1000).Image acquisition was done with an AxiObserver A1 Microscope (Zeiss ...
• Differential chemoarchitecture of Purkinje neurons contributes to intrinsic firing properties

  Cheryl Brandenburg, et al., bioRxiv - Neuroscience 2021

  Quote: ... but after rinsing sections were incubated in secondary antibodies (donkey anti-guinea pig 555 1:1,000 Sigma SAB4600298; goat anti-mouse 546 1:400 Invitrogen A-11030; goat anti-mouse 488 Invitrogen 1:400 A-11001) for three hours ...
• Peptide vaccine candidate mimics the heterogeneity of natural SARS-CoV-2 immunity in convalescent humans and induces broad T cell responses in mice models

  Eszter Somogyi, et al., bioRxiv - Immunology 2020

  Quote: ELISAs for mouse studies were performed for the quantitative measurement of total mouse IgG production in plasma samples using IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, #88-50400-22) for BALC/c cohorts and IgG (Total ...
• Dynein-mediated trafficking and degradation of nephrin in diabetic podocytopathy

  Hua Sun, et al., bioRxiv - Physiology 2022

  Quote: ... the transcription data for dynein components was retrieved from the Hodgin Diabetes Mouse Glom dataset (2013, Affymetrix Mouse Genome 430 2.0 Array platform)32 ...
• Single-cell image-based genetic screens systematically identify regulators of Ebola virus subcellular infection dynamics

  Rebecca J. Carlson, et al., bioRxiv - Microbiology 2024

  Quote: ... Cells were immunostained with an anti-EBOV GP mouse antibody (IBT, MD, USA) and Alexa Fluor 488 anti-mouse secondary (Thermo Fisher, MA, USA). Nuclei were counterstained with Hoechst 33342 ...
• MiniBAR/KIAA0355 is a dual Rac and Rab effector required for ciliogenesis

  Ronan Shaughnessy, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1:500), anti-mouse IgG2b Alexa Fluor 488 (In-vitrogen A21141, 1:500) and anti-mouse IgG1 Alexa Fluor 594 (Invitrogen A21125, 1:500). The following antibod-ies were used for western blots of human cell extracts ...
• Increased hyaluronan by naked mole-rat HAS2 extends lifespan in mice

  Zhihui Zhang, et al., bioRxiv - Physiology 2023

  Quote: Thirty-six cytokines and chemokines were measured in young and old mouse plasma by luminex multiplex technique using a Cytokine & Chemokine 36-Plex Mouse ProcartaPlex™ Panel 1A kit (Thermo Fisher Scientific). The luminex multiplex assay was performed using undiluted plasma samples following the manufactures instruction.
• RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds

  Bin Fu, et al., bioRxiv - Biophysics 2023

  Quote: ... The sections were then washed three times for five minutes in PBS followed by the corresponding AlexaFluor secondary antibodies (anti-mouse 568—A11031, Thermo Fisher, anti-rabbit 568—A11011, Thermo Fisher, anti-mouse 488—A11001, Thermo Fisher, anti-rabbit 488—A11008 ...
• Ultra-High Dose Rate Helium Ion Beams: First In Vivo Evidence for Neuroprotective FLASH Effect

  Ivana Dokic, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 0.1% Triton X in phosphate-buffered saline (phosphate-buffered saline (PBS)) and ReadyProbesTM Mouse-on-Mouse IgG Blocking Solution (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
• Coupling cellular drug-target engagement to downstream pharmacology with CeTEAM

  Nicholas C.K. Valerie, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-V5 tag (mouse monoclonal, 46-0705) and anti-V5 tag (mouse monoclonal, MA5-15253) was purchased from Invitrogen (now Thermo Fisher Scientific). anti-NUDT5 (rabbit polyclonal ...
• Systemic ligand-mimicking bioparticles cross the blood-brain barrier and reduce growth of intracranial triple-negative breast cancer using the human epidermal growth factor receptor 3 (HER3) to mediate both routes

  Felix Alonso-Valenteen, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
• PSMA-bearing extracellular vesicles secreted from prostate cancer convert the microenvironment to a tumor-supporting, pro-angiogenic state

  Camila Maria Longo Machado, et al., bioRxiv - Cancer Biology 2022

  Quote: ... the slices were incubated with a secondary goat-anti-mouse or rabbit Alexa-488 or Alexa 563 antibodies or goat-anti-mouse-HRP (Invitrogen, Life Technologies, Carlsbad, CA) for 2 h followed by DAPI cell nuclei staining or Hematoxylin staining ...
• Exploring the lncRNA localization landscape within the retinal pigment epithelium under normal and stress conditions

  Tadeusz J. Kaczynski, et al., bioRxiv - Genetics 2022

  Quote: ... incubated in secondary antibody (either Alexa Fluor 488 goat anti-mouse [Fisher Scientific, Cat #: A11029] or Alexa Fluor 555 donkey anti-mouse [Fisher Scientific, Cat #: A31570]) for 1 hour at room temperature ...
• The Golgi Glycoprotein MGAT4D is an Intrinsic Protector of Testicular Germ Cells From Mild Heat Stress

  Ayodele Akintayo, et al., bioRxiv - Developmental Biology 2019

  Quote: ... labeling and hybridization to a mouse Affymetrix Clariom™ D array previously known as GeneChip™ Mouse Transcriptome Array 1.0 (Affymetrix, Santa Clara, CA). Raw intensity data (.CEL files ...
• Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components

  Neal I. Callaghan, et al., bioRxiv - Bioengineering 2022

  Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
• Staphyloxanthin production by Staphylococcus aureus promotes resistance to oxidative stress to delay diabetic wound healing

  Amelia R. McCready-Vangi, et al., bioRxiv - Microbiology 2022

  Quote: ... Staining for surface markers was performed by incubating the cells in single cell suspension with anti-mouse CD16/CD32 mouse Fc block (Thermo Fisher Scientific, RRID: AB_467135) and 10% rat-IgG in PBS ...
• Investigating EMT-mediated resistance to EGFR tyrosine kinase inhibitors in NSCLC using innovative organoid models

  Nobuaki Kobayashi, et al., bioRxiv - Cancer Biology 2024

  Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
• Rem2 interacts with CaMKII at synapses and restricts long-term potentiation in hippocampus

  Rabia Anjum, et al., bioRxiv - Neuroscience 2024

  Quote: ... The sections were then incubated in the secondary antibody (goat anti-mouse Alexa Fluor™ 546, Thermofisher A-21052 or goat anti-mouse Alexa Fluor™ 633, Thermofisher A-21052) at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories ...
• Aberrant activation of the innate immune sensor PKR by self dsRNA is prevented by direct interaction with ADAR1

  Ketty Sinigaglia, et al., bioRxiv - Immunology 2023

  Quote: ... A secondary antibody was used in TBS-T with a dilution of 1:5,000 for Anti-Mouse (Thermo Fisher, Goat anti-Mouse IgG, #31430) and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG ...
• Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase

  Paul Batty, et al., bioRxiv - Cell Biology 2023

  Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
• Loss of the extracellular matrix protein Perlecan disrupts axonal and synaptic stability during Drosophila development

  Ellen J. Guss, et al., bioRxiv - Neuroscience 2023

  Quote: ... Iowa City, IA), mouse anti-Futsch (22C10 DSHB, Iowa City, IA) and mouse anti-GFP at 1:1000 (#A-11120, Thermo Fisher Scientific, Waltham, MA). Secondary antibodies used in this study were goat anti-mouse Alexa Fluor 488-conjugated IgG at 1:500 (#A-32723 ...
• Influenza A virus during pregnancy disrupts maternal intestinal immunity and fetal cortical development in a dose- and time-dependent manner

  Ashley M. Otero, et al., bioRxiv - Neuroscience 2023

  Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
• A role for neutral variation in the evolution of C₄ photosynthesis

  Shanta Karki, et al., bioRxiv - Plant Biology 2020

  Quote: ... and the Alexa Fluor 488 goat anti-mouse IgG (Invitrogen) secondary antibody ...
• RIPK3 facilitates host and pathogen interactions after oral Toxoplasma gondii infection

  Patrick W. Cervantes, Laura J. Knoll, bioRxiv - Microbiology 2020

  Quote: ... followed by Alexa Flour 488 anti-mouse (Thermo Fisher, Z25002) secondary antibody for 1 hour ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Alexa Fluor 568 donkey anti-mouse IgG (Thermofisher A-10037), Alexa Fluor 568 donkey anti-mouse IgG1 (Thermofisher A-21124) ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Alexa Fluor 488 donkey anti-mouse IgG (Thermofisher A-21202), Alexa Fluor 488 donkey anti-mouse IgG2a (Thermofisher A-21131) ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Alexa Fluor 488 donkey anti-mouse IgG2a (Thermofisher A-21131), Alexa Fluor 568 donkey anti-rabbit IgG (Thermofisher A-10042) ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Alexa Fluor 568 donkey anti-mouse IgG1 (Thermofisher A-21124), Alexa Fluor 647 donkey anti-rabbit IgG (Thermofisher A-31573) ...
• SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

  Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

  Quote: ... Alexa Fluor 647 donkey anti-mouse IgG (Thermofisher A-31571). ALL Alexa fluor secondary antibodies were used at 1:1000.
• Pre-neoplastic stromal cells drive BRCA1-mediated breast tumorigenesis

  Kevin Nee, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse anti-KRT 18 (1:10, Invitrogen, Carlsbad, CA, USA); mouse anti-KRT 14 (1:10 ...
• Proton export drives the Warburg Effect

  Shonagh Russell, et al., bioRxiv - Cancer Biology 2021

  Quote: ... or anti-mouse-Alexa Fluor 594(1:2000; A11005, Invitrogen) and additionally incubated in WGA ...
• Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis

  Samantha C. Schwager, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and AlexaFluor 568 conjugated to donkey anti-mouse (Life Technologies). Actin was stained using Texas Red Phalloidin (Life Technologies).
• INTS13 Mutations Causing a Developmental Ciliopathy Disrupt Integrator Complex Assembly

  Lauren G. Mascibroda, et al., bioRxiv - Biochemistry 2020

  Quote: ... and goat anti-mouse Alexa Fluor 488 (Life Technologies, A11029), followed by three more washes with PBS ...
• Histone Acetyltransferase 1 is Required for DNA Replication Fork Function and Stability

  Paula A. Agudelo Garcia, et al., bioRxiv - Biochemistry 2020

  Quote: ... and AlexaFluor 488–conjugated anti-mouse (1:250, Molecular Probes) were used as secondary antibodies and incubated for 1 hour at room temperature ...
• Proximity-dependent biotinylation to elucidate the interactome of TNK2 non-receptor tyrosine kinase

  Raiha Tahir, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Alexa Fluor 488 goat anti-mouse IgG (Invitrogen, A-11029), Alexa Fluor 568 goat anti-rabbit IgG (Invitrogen ...
• Phospholipases D1 and D2 regulate cell cycling in primary prostate cancer cells and are differentially associated with the nuclear matrix

  Amanda R Noble, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and a goat anti-mouse Alexa Fluor 488 (A11029, Thermofisher).
• Single-cell landscapes of primary glioblastomas and matched organoids and cell lines reveal variable retention of inter- and intra-tumor heterogeneity

  VG LeBlanc, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Mouse anti-Alpha-Smooth Muscle Actin (ACTA2) (ThermoFisher; 1:100), Rabbit anti-PDGFR beta (Abcam ...
• Dual G9A and EZH2 inhibition stimulates an anti-tumour immune response in ovarian high-grade serous carcinoma

  Pavlina Spiliopoulou, et al., bioRxiv - Cancer Biology 2021

  Quote: ... along with 1 ng/mL of mouse IFNγ (ThermoFisher #PMC4031). After 72 hours ...
• Uropathogenic E. coli induces DNA damage in the bladder

  Camille V. Chagneau, et al., bioRxiv - Microbiology 2020

  Quote: ... and Alexa 488 Goat anti-Mouse antibody (Life technologies A11029)) and DAPI ...
• Loss of Activity-Induced Mitochondrial ATP Production Underlies the Synaptic Defects in a Drosophila model of ALS

  Nicholas E. Karagas, et al., bioRxiv - Neuroscience 2021

  Quote: ... and Alexa Fluor 568-conjugated anti-mouse secondary antibodies (ThermoFisher) at room temperature for 1.5 hours ...
• BET Inhibition Enhances TNF Mediated Anti-Tumor Immunity

  Lisa C. Wellinger, et al., bioRxiv - Cancer Biology 2021

  Quote: ... or Mouse IgG1 Isotype Control (MOPC-21) (ThermoFisher #MA1-10407) were added to the co-culture where indicated.
• EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells

  Irini Topalidou, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-transferrin receptor (1:1000, ThermoFisher, #13-6800), Membranes were stained with the relevant secondary antibodies in 3% milk in TBST ...
• EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells

  Irini Topalidou, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-transferrin receptor (1:100, ThermoFisher, #13-6800), mouse monoclonal anti-syntaxin 6 (1:100 ...
• Fibrin fragment E potentiates TGF-β-induced myofibroblast activation and recruitment

  Peder Fredlund Fuchs, et al., bioRxiv - Cell Biology 2019

  Quote: ... Secondary antibodies used were: goat-anti-mouse alexa 680 (Invitrogen) and goat-anti-rabbit IRDye 800 (Rockland ...
• Mechanisms of up-regulation of Ubiquitin-Proteasome activity in the absence of NatA dependent N-terminal acetylation

  Ilia Kats, et al., bioRxiv - Cell Biology 2020

  Quote: ... followed by probing with mouse anti-Pgk1 (22C5D8, Molecular Probes) and imaging on a LAS-4000 system (Fuji).
• Mechanisms of up-regulation of Ubiquitin-Proteasome activity in the absence of NatA dependent N-terminal acetylation

  Ilia Kats, et al., bioRxiv - Cell Biology 2020

  Quote: ... followed by probing with mouse anti-Pgk1 (22C5D8, Molecular Probes) and goat anti-mouse HRP (115-035-003 ...
• Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration

  Francisco J. Calero-Cuenca, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-Myc 1:200 (Alfagene/Life Technologies #13-2500). The secondary antibodies (1:600 dilution ...