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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740). Libraries were prepared using the Kapa RNA HyperPrep Kit with RiboErase (Roche #08098131702) ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM sodium pyruvate and 100 U mL−1 penicillin and 100 µg mL−1 streptomycin (Thermo Fisher Scientific) at 37°C and 5% CO2 in a humidified incubator.
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bioRxiv - Biophysics 2024Quote: ... cells transduced with the pLKO.1 puro backbone or sg-Bag6 1 with Ef1alpha Puro-P2A-BFP(1)(1) backbone were cultured in medium containing 0.5 µg/ml Puromycin (Gibco).
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bioRxiv - Microbiology 2024Quote: ... with 7.5% heat-inactivated Fetal Calf Serum (FCS; Life Technologies with 1% penicillin/streptomycin PS, 5000U.mL−1 and 5000µg.mL−1 respectively (Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1% Triton X, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1× Halt Protease Inhibitor Cocktail from Thermo Scientific, 1x Phosphatase Inhibitor Cocktail from Cell Signaling Technology) ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked in 5% milk for 1 hour at RT prior to incubation with primary antibodies (Muc2: 1:1000 from Proteintech, IRE1β: 1:500 and AGR2: 1:1000 from Invitrogen, and Fmo5: 1:1000 from Cell Signalling) overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then washed three times with PBS containing 1% BSA followed by 1 h incubation with donkey anti-mouse Alexa Fluor 488-conjugated secondary antibody (1:1000, ThermoFisher) at 4°C in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The final pellet was resuspended in advanced DMEM/F12 supplemented with 1% Glutamax/1% HEPES/1% penicillin/streptomycin (all Gibco) and the desired amount of cell suspension mixed with Basement Membrane Extract (Type 2 ...
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bioRxiv - Genomics 2020Quote: ... 100 ng of total RNA spiked with 1 μl of 1:500 diluted ERCC Mix 1 (Life Technologies, Carlsbad, CA) was used to start cDNA synthesis ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2019Quote: ... were cultured in (DMEM containing D-glucose [1 g L-1] and sodium pyruvate [110 mg L-1] (Life Technologies), supplemented with penicillinstreptomycin [100 U mL-1] (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... the apical and basolateral sides were gently washed three times with 1xDPBS and the filters were 1% PFA fixed and stained with polyclonal rabbit anti-zona occludens-1 (ZO-1) antibody (Invitrogen, Rockford ...
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bioRxiv - Bioengineering 2020Quote: CellTrace™ prelabeled cells from encapsulations were removed from the well plate surface by a sterile spatula and at least three gels were batched per sample into an enzymatic digestion solution consisting of 1:1 solution of HBSS (calcium and magnesium supplemented) and 1% bovine serum albumin in PBS with 1 µM HEPES (Gibco), 300 U/mL collagenase type IV (Worthington Biochemical) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 cells were stained with NCAM-1 antibody (CD56 Anti human Alexafluor 700, 1:200 dilution, Fisher Scientific, BDB557919) for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244; goat anti-rabbit 568 1:200, Invitrogen A11011 ...
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bioRxiv - Biochemistry 2020Quote: ... which consists of DMEM/F12 and Neurobasal medium (1:1) with 1% B27 supplement (Thermo Fisher Scientific, Carlsbad, CA, USA). The medium was changed every five days for 30 days and consisted of the removal of half of the content and addition of this same volume of fresh medium ...
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bioRxiv - Bioengineering 2021Quote: ... and enzymatically digested in a solution consisting of 1:1 solution of HBSS (calcium and magnesium supplemented) and 1% bovine serum albumin in PBS with 1 μM HEPES (Gibco), 300 U/mL collagenase type IV (Worthington Biochemical) ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% FBS (CellMax, Lanzhou Minhai Bio-Engineering, Lanzhou, China) and RPE-1 cells (ATCC, CRL-4000) in DMEM/F12 (1:1) (GIBCO) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... the inoculum was removed, and 2 mL overlay media was applied (MEM with 10% FBS containing 1% methylcellulose, 1% HEPES, 1% GlutaMAX (Gibco), and 1% Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) was performed for 1 h at 37°C in combination with 1 μM HCS cell mask (Invitrogen; H32720) that stains the entire cell ...
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bioRxiv - Microbiology 2022Quote: ... a-tubulin (1:500, 2144, Cell Signalling), acetyl-a-tubulin (1:500, 1215, Cell Signalling) GAPDH (1:10,000, G9545, Invitrogen, Fisher Scientific) and GAPDH (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus was produced by transfecting all plasmids at a 1:1:1 molar ratio (30 μg total DNA) into HEK293T cells with Lipofectamine3000 (Invitrogen). Viral particles were collected at 72 hours by filtering spent medium through a 0.45 μm cellulose acetate filter (Millipore Sigma) ...
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bioRxiv - Immunology 2022Quote: ... were harvested bilaterally into cold complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Cell Biology 2022Quote: ... slices were labelled with SPY650-DNA (1:1000, #SC501, Spirochrome) for 1 h and CellTraceTM CFSE (1:1000, #C34554, ThermoFisher) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was added at a ratio of 1:100 and incubated for at least 1 h at room temperature while the secondary antibody (1:300 Invitrogen) was allowed to incubate with the sample for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Microbiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) at 1 μg mL−1 concentration and 50 μL of Film Tracer 1-43 membrane marker (Invitrogen) at 1 μg mL-1 concentration in DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... N2B27 (DMEM/F12 [Gibco 11320-074], and Neurobasal in 1:1 ratio [Gibco 21103-049], with 1:200 N2 Supplement [Gibco 17502-048] ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant after enzymatic reaction was diluted (1:300) in methanol:water (1:1) and injected into a Dionex UltiMate 3000 LC system (Thermo Scientific) with a ZIC-pHILIC (150 mm x 4.6 mm ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were detected by probing with biotin-conjugated anti-rat antibody (1:1,000) and Alexa 488-conjugated anti-human antibody (1:1,000) followed by Alexa 594-conjugated streptavidin (Invitrogen, 1:1000). Slides were then stained with 1ug/mL DAPI ...
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bioRxiv - Cancer Biology 2022Quote: 22RV1 shSCRM and 22RV1 shMALAT1 cells (1 × 104) were plated in a serum-free stem cell medium consisting of DMEM-F12 (1:1, Invitrogen), 1X B27 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30, 1:250, Invitrogen; Rabbit anti-MyosinVIIa ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Developmental Biology 2019Quote: ... 1% low melting point (LMP) agarose (ThermoFischer) was prepared as 1:1 v/v ratio of 1 × PBS and Schneider’s medium (ThermoFisher 21720024) then pipetted onto a 3 cm Petri dish (MatTek ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were subjected to incubation with secondary antibody (1:400 anti-rabbit Alexa 568, 1:400 anti-rabbit Alexa 488, 1:250 anti-mouse Alexa 594 from Invitrogen) for 1.5 hours followed by three washes with 1X PBS for 10 minutes each ...
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bioRxiv - Developmental Biology 2020Quote: ... After washing with PBS guts were incubated with secondary antibodies (1:500 Goat anti-MouseAlexa647 [Invitrogen]; 1:500 Goat anti-RatAlexa647 [Invitrogen]; 1:500 Goat anti-RabbitAlexa647 [Invitrogen]) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405, 1:1000, Abcam; donkey anti-rabbit 555, 1:1000, ThermoFisher). After four final washes ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 3% BSA in PBS with 0.1% Tween20 for 1 hour and immunostained with rabbit anti-nestin (1:200, PA-1-86334; Invitrogen, USA), rabbit SOX2 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used: anti-ZO-1 (Zymed, #40-2200, 1:100 or Invitrogen, #33-9100, 1:100); anti-N-cadherin (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000, goat α-rat (AF488, RRID:AB_2534074) at 1:2000, goat α-mouse (AF594, RRID:AB_141372) from Invitrogen (1:2000). Images were quantified using FIJI software (National Institutes of Health ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were counted and cryopreserved at a concentration of 1×108 cells/ml in 1:1 RPMI and PBMC freezing medium of 85% fetal bovine serum (Gibco) and 15% dimethyl sulfoxide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Microbiology 2022Quote: ... mice were given a mixture of antibiotics (ampicillin 1 mg/mL, vancomycin 0.5 mg/mL, neomycin 1 mg/mL, metronidazole 1 mg/mL; Fisher Scientific) in drinking water for 3 consecutive days (Figure 2G).
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bioRxiv - Biochemistry 2022Quote: ... 1:100 dilution of mouse anti- alpha 1 Spectrin (ab11751) and 1:500 dilution of rabbit anti-KCNN4 (76647, Invitrogen). For co- immunostaining experiments with rabbit primary antibodies ...