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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for Recombinant Human UBE2Z Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were loaded equally in terms of protein concentration into 10% Bis-Tris protein gels (Life Technologies) and blotted on nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2020Quote: ... Prewashed beads (50% protein A and 50% protein G, catalog numbers 10002D and 10004D, respectively, Thermo Fisher) were added to the immunocomplex and incubated for 30 minutes at room temperature with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was quantified with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, catalog no. 23225). Approximately 50 μg of total protein was loaded and separated on 12% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: OMVs were quantified by total protein dosage with the Pierce® BCA Protein Assay Kit (Thermo Scientific). OMVs were then analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Neuroscience 2021Quote: ... Cell surface proteins were biotinylated and isolated using Pierce Cell Surface Protein Isolation Kit (#89881, Thermo Fisher). All the steps were performed at 4°C unless otherwise mentioned ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were clarified by centrifugation and then quantified using the Pierce BCA Protein Assay kit (ThermoFisher). 20μg of protein extracts were subjected to SDS-PAGE and then transferred onto a PVDF membrane ...
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bioRxiv - Bioengineering 2020Quote: Target proteins were immobilized to magnetic beads by first coating protein G magnetic beads (ThermoFisher Scientific, 10004D) with anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented chromatin was then pre-cleared with 5 μl of Dynabeads Protein A/Protein G (ThermoFisher) and immunoprecipitated overnight at 4 °C with the pre-prepared antibody-beads complex ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).
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bioRxiv - Synthetic Biology 2019Quote: ... and cell lysate protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The reaction mixture for CFPS consisted of the following components in a final volume of 8–400 μL containing 1.2 mM ATP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
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bioRxiv - Neuroscience 2019Quote: ... and equal amounts of protein were then loaded on precast NuPage 10% Bis-Tris protein gels (Invitrogen). After electrophoretic separation ...
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bioRxiv - Neuroscience 2020Quote: ... Protein content was measured using the Pierce 660 nm protein assay reagent (Thermo Scientific, Waltham, MA, USA). Equal amounts of proteins were separated by denaturing electrophoresis in Mini-Protean TGX stain-free gels (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration of the clarified lysate was determined using Pierce BCA Protein Assay (Thermo Fisher Scientific, 23225), after which it was reduced by 100 mM dithiothreitol at room temperature for 30-60 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of protein-containing supernatant was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). 10ug of each sample was loaded onto NuPage 4-12% Bis-Tris protein gels (Thermo Fisher ...
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Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in Clostridioides difficile R20291bioRxiv - Microbiology 2021Quote: ... The intracellular total protein concentration was measured by using a BCA protein assay (Thermo Scientific, Suwanee, GA), and normalized to the same protein concentration for intracellular toxin analysis by ELISA ...
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bioRxiv - Immunology 2020Quote: Protein concentration of EVs was measured using the BCA assay (Pierce BCA Protein Assay Kit, ThermoFisher Scientific). EVs or S-Trim preparations were separated by SDS-PAGE on a 4−15% acrylamide gel (4−15% Mini-PROTEAN® TGX Stain-Free™ Gel ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by SDS–PAGE electrophoresis with a 4–12% Bis-Tris protein gel (Thermo Scientific) and then transferred to an Immobilon-FL membrane (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total protein was measured using the Qubit total protein quantification kit and Qubit 2.0 fluorometer (Thermo Scientific). Cytokines were measured using a Milliplex immunoassay kit (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... supernatants were collected and protein concentration were measured using BCA Protein Assay Kit (Thermo Fisher Scientific, Pierce). Concentration of IL-1β ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was collected and protein concentrations were measured with the Qubit Protein Assay kit (Life Technologies). Protein samples were diluted in 4x sample buffer (4x Laemmli buffer + β-mercaptoethanol) ...
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bioRxiv - Molecular Biology 2020Quote: To determine protein concentrations the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) was used ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration of tissue homogenate was assayed using Pierce BAC Protein Assay Kit (Thermo Scientific, catalog 23227). Uncoupling protein (UCP ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration of ileal extract was determined by Pierce BCA Protein assay (Thermo Scientific, Waltham, MA, USA,) according to manufacturer’s instructions and 96-well NUNC- Maxisorp plates (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration of tissue homogenate was assayed using Pierce BAC Protein Assay Kit (Thermo Scientific, catalog 23227). Uncoupling protein (UCP ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of the protein was determined using a BCA protein assay (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Physiology 2021Quote: ... and the protein content of the supernatant was determined using Pierce BCA Protein Assay Kit (Thermo Scientific). Cystine content was measured using HPLC-MS/MS as described previously.20 A volume of 5 ml of internal standard (20 mM cystine-D4 ...
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bioRxiv - Physiology 2020Quote: ... and protein content was estimated using Pierce 660nm protein assay reagent (Thermo Fisher Scientific, Cat no. 22660). Equal amount of protein was loaded onto SDS-PAGE gels for separation ...
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bioRxiv - Neuroscience 2019Quote: ... and anti-glucose-regulated protein of 78 kDa / binding immunoglobulin protein (GRP78/BiP) (PA1-014A, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was kept and the protein concentration quantified using the Qubit™ Protein Assay (Invitrogen, Q33212). 30 μg of protein was used per lane ...
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bioRxiv - Bioengineering 2021Quote: ... Total protein concentration was determined using the bicinchoninic acid assay (BCA Protein Assay Kit; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were measured using a Pierce 660 nm protein Assay kit (Thermo Fisher Scientific, Rockford, USA). Proteins (100 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration of the samples was measured with BCA protein assay kit (Thermo Fisher Scientific, Massachusetts, USA) and quantified with GloMax microplate reader (Promega GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... The final protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific Cat. #: 23227). Protein concentration was adjusted to 10 μg/100 μL in 50 mM ammonium bicarbonate buffer ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration of the OMVs was quantified using a BCA Protein assay kit (Thermo Fisher Scientific). To ensure that the suspension containing OMVs was free of bacteria ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 500 µg of protein were then incubated with Protein G Dynabeads (Thermo Fisher Scientific) and 2 µg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2021Quote: ... Sample protein content was normalized using the Pierce™ BCA protein assay kit (Thermo Scientific™, 23225). LDS (Invitrogen™ ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
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bioRxiv - Plant Biology 2021Quote: ... 2µg protein (crude extract, determined by PierceTM BCA Protein Assay Kit, Thermo Fisher Scientific, Waltham, Massachusetts, US) was used for the EMSA ...
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bioRxiv - Pathology 2021Quote: ... Protein concentration for each sample was determined using a Micro BCA™ Protein Assay Reagent Kit (ThermoFisher) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein per sample was determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins were denatured using 1 x NuPAGE® LDS sample buffer (Thermo Fisher Scientific ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... Protein-antibody complexes were then captured with the Pierce™ Protein A/G Agarose (ThermoFisher Scientific, #20421) at 4°C for 2 hrs with rotation and beads were then rinsed with wash buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). 10 μg of protein was run on a SDS-PAGE gel until they entered the separation gel ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the supernatant was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Protein concentration of tissue lysate samples was determined by Pierce® 660 nm Protein Assay (Thermo Scientific). 40-50µg total protein of each tissue lysate sample and 20ug of cell lysate sample was added to 3.75µL4x NuPAGE buffer (106mM Tris HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitation of protein complexes was done as described in17 using Protein A beads (Thermo Fisher Scientific, #10008D) coupled with HA antibody (CST ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein concentration was determined by Pierce BCA protein assay reagent kit (catalog #23227; Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined in parallel using a Pierce®Microplate BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was determined by Bradford dosage with Pierce BCA protein assay kit (Thermo scientific #23225). Ten microgram of total protein of hippocampus and muscle extracts were electrophoretically separated in SDS–polyacrylamide gels (10% ...