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Citations for Thermo Fisher :
5701 - 5750 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Genetics 2019Quote: ... Thirty-two libraries of 100 pM were mixed together and emulsion PCR amplified on the Ion PI™ Hi-Q™ ISPs using the Ion OneTouch™ 2 Instrument (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Template preparation was performed by the Ion PGM Hi-Q View kit on the Ion OneTouch™ 2 System (Life Technologies, Grand Island, NY, United States) and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Cell Biology 2020Quote: The breast cancer cell line MDA-MB-468 was cultured at 37°C and 5% CO2 in RPMI medium supplemented with 10% (v/v) fetal calf serum (FCS) and Antibiotic-Antimycotic (Thermo Fisher Scientific, Schwerte, Germany). The keratinocyte cell line HaCaT ...
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bioRxiv - Cell Biology 2019Quote: ... Live-cell imaging experiments were performed using 8-well ibidi chambers (Vitaris, Switzerland) and imaging medium (Leibovitz L-15 medium from Thermofisher, supplemented with 10 % FCS). The following drug concentrations were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Japan) and was measured at an optical density (OD) of 450 nm with a Multiskan FC microplate photometer (Thermo Fisher Scientific, Waltham, MA, USA). For the cell proliferation assay ...
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bioRxiv - Immunology 2020Quote: Analysis of IgG Fc-glycosylation was performed with nanoLC reverse phase (RP)-electrospray (ESI)-MS on an Ultimate 3000 RSLCnano system (Dionex/Thermo Scientific, Breda, The Netherlands) coupled to an amaZon speed ion trap MS (Bruker Daltonics ...
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bioRxiv - Immunology 2020Quote: ... Briefly, TILs were cultured in RPMI 1640 culture medium (SERO-Med GmbH, Wien) supplemented with 10% FCS HyClone (Gibco Laboratories, Grand Island, NY, USA) and stimulated for 5 h using the Leukocyte Activation Cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... Prostate cancer cell lines were cultured in RPMI medium supplemented with 2 mM L-glutamine and 10% heat-inactivated FCS (ThermoFisher Scientific, Melbourne, VIC, Australia). Cell media was changed every three days and cells were passaged regularly at ∼80-90% confluency by trypsinization ...
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bioRxiv - Cell Biology 2022Quote: ... the confocal volume was determined by performing FCS using a dye with known diffusion coefficient and concentration (Alexa Fluor 488 NHS ester; Thermo Fisher Scientific for mEGFP). To convert fluorescence intensity to the concentration ...
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bioRxiv - Immunology 2019Quote: Analysis of IgG Fc-glycosylation was performed with nanoLC reverse phase (RP)-electrospray (ESI)-MS on an Ultimate 3000 RSLCnano system (Dionex/Thermo Scientific, Breda, The Netherlands) coupled to a Maxis HD quadrupole-time-of-flight MS equipped with a nanoBooster (Bruker Daltonics ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300, Thermofisher Scientific, Clone 30-F11), anti-LY6G-V450 (1:300 ...
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bioRxiv - Microbiology 2022Quote: ... Staining for surface markers was performed by incubating the cells in single cell suspension with anti-mouse CD16/CD32 mouse Fc block (Thermo Fisher Scientific, RRID: AB_467135) and 10% rat-IgG in PBS ...
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bioRxiv - Microbiology 2023Quote: The growth burden imposed by lateral flagella was determined in an automated growth experiment in a MultiskanTM FC microplate photometer (Thermo Scientific, software SkanIt 4.1). The wells of a 96-well microplate were filled with 200 μl of either MB alone (blank wells and border wells ...
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bioRxiv - Immunology 2023Quote: ... The fragment antigen-binding (Fab) part was separated from undigested IgG and the crystallizable fragment (Fc) using an Immobilized Protein A column (Thermo Fisher Scientific, No. 20356). The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... to a microplate for the absorbance measurement at 765 nm with a Varioskan Flash plate reader (Multiskan FC Microplate Photometer, Thermo Scientific, Waltham, MA, USA). A calibration curve of the standard compound gallic acid with a linearity range from 50 mg/L to 800 mg/L was constructed ...
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bioRxiv - Immunology 2023Quote: Mouse peripheral blood was processed to remove RBC via lysis prior to Fc blocking and then stained with antibodies against surface markers including biotin-CD93 (Invitrogen, cat # 13-5892-85), BV605-CD19 (BD ...
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bioRxiv - Immunology 2023Quote: ... the cultured B cells were stained with Fc block followed by fixable viability dye eFluor 780 and FITC BAFF-R monoclonal antibody (Invitrogen; cat #11-5943-81) and analyzed on a LSRFortessa X-20 flow cytometer (BD).
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 100µg/ml pHrhodo Green Dextran-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes and melanoma cells were lysed in Pierce RIPA buffer (Thermo Scientific) containing 1× Halt protease inhibitor cocktail (100× ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CRC organoids medium is composed of Ad-DF+++ (Advanced DMEM/F12 (GIBCO) supplemented with 2 mM Ultraglutamine I (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: Primary human umbilical vein endothelial cells (HUVECs) were obtained from Gibco (Cat#: C0035C) and cultured in Medium 200 (Cat# ...
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bioRxiv - Cell Biology 2020Quote: ... HIF1A levels were measured in duplicates applying a HIF1A Human ELISA kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Cell Biology 2020Quote: Human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Gibco, #A3349401) and passaged with EDTA (Life Technologies #15575-020) ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Genetics 2019Quote: ... RPE1 cells and primary human fibroblasts were cultivated in complete DMEM medium (Gibco).
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bioRxiv - Molecular Biology 2021Quote: Human MSCs were maintained in growth media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing D-glucose (1g/L) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher). Templating and sequencing were performed with the Ion PI HiQ OT200 kit ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Perforin eF450 (1:100, clone dG9, Invitrogen, cat # 48-9994-42). Cells were analyzed on a BD LSR Fortessa flow cytometer ...
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bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
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bioRxiv - Immunology 2021Quote: After 48 h of stimulation with Human T-activator CD3/CD28 Dynabeads (ThermoFisher) CD4+ lymphoblasts were recovered for transfection of CRISPR/Cas9 nucleoprotein complexes ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and 10 µl/ml Fibronectin from human plasma (Thermo Fisher Scientific, #33016-015)] for 2-3 h at 37 °C/5% CO2 and stored at 4 °C after thorough removal of coating solution.
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bioRxiv - Physiology 2019Quote: Human embryonic kidney (HEK-293) cells were cultured in DMEM (Gibco, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: We used GeneChip Human Exon 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) to measure the expression levels of transcripts in each brain tissue specimen ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse and human PSCs were cultured in DMEM (10-013-CV; Fisher Scientific) containing 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... and sCD163 (Thermo Scientific™ Pierce™ Human CD163 Kit, Thermo Scientific, USA) in plasma.
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bioRxiv - Immunology 2020Quote: ... Human embryonic kidney (HEK293T) cells and HeLa cells were cultured in DMEM (Gibco) supplemented with 100U/ml Penicillin (Sigma-Aldrich),100μg/ml Streptomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... T Cells were stimulated with Dynabeads™ Human T-Expander CD3/CD28 (Gibco). The cells were cultured in GRex (Wilson Wolfe ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Cells were incubated with AF488 goat anti-human IgG1 (1:500, A10631, Invitrogen) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Amyloid Beta-40 ELISA was performed using the Human AB40 ELISA Kit (Invitrogen). Secretion values were normalized to protein content of wells as measured by RIPA harvest and BCA protein quantification.
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bioRxiv - Immunology 2020Quote: ... T cells were stimulated with Dynabeads® Human T expander CD3/CD28 (Invitrogen) at a 1:3 ratio (T cell:bead) ...
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bioRxiv - Genomics 2019Quote: Human colon carcinoma cells HT-29 were cultured in 1640 medium (Gibco, USA) supplemented with 10% (v/v ...