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Citations for Thermo Fisher :
5701 - 5750 of 7824 citations for HER4 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... left in resting state or activated with Dynabeads Human T-Activator CD3/CD28 (1161D, Thermo Fisher Scientific) for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-Be(2c) and SK-N-SH human neuroblastoma cells were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic lung fibroblasts (MRC-5, European Collection of Cell Cultures) were maintained in DMEM (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: ... All human primary cell lines were cultured in DMEM high glucose supplemented with GlutaMAX (Gibco, 10566-016), with further addition of 15% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... followed by PBS washing and immunostaining with secondary AlexaFluor633 anti-human antibodies (Thermo Fisher Scientific, #A-21091) for 30 mins at 4°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: sEV isolated from human HTLV/T cell cultures with Total Exosome Isolation Reagent (#4478359, Thermo Fisher Scientific) were solubilized in SDS buffer ...
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bioRxiv - Biochemistry 2024Quote: ... human full-length VPS35L (untagged, synthesized as a codon-optimized GeneString from Thermo Fisher to improve expression), VPS26C (untagged) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). For comparing tumor cell growth in different groups ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA hybridized to a Mouse Clariom S Human Transcriptome Assay microarray plate (Affymetrix, Santa Clara, CA) and the plate was scanned by a GeneTitan MC Instrument (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Immunology 2023Quote: Immortalized human SZ95 sebocytes (Zouboulis et al., 1999) were cultured in T-75 culture flasks (Thermo Scientific) with Sebomed basal medium (DMEM/F12 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once and then incubated with Alexa Fluor 488 goat anti-human IgG antibody (ThermoFisher) diluted 1/200 in PBS + 1% FCS or BB buffer ...
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bioRxiv - Cell Biology 2023Quote: The release of CXCL10 to the culture medium was detected using Human ProcartaPlex immunoassays (Invitrogen, Vienna, Austria) following the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Human embryonic kidney 293T cells were grown in Complete DMEM (Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 8 µg of cDNA was fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Hepatic gene expression was measured using Affymetrix Human Exon 1.0 ST v1 Array (Affymetrix, Santa Clara, CA) in adolescents and Illumina Human-6 Expression Bead Chips (Illumina ...
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bioRxiv - Bioengineering 2022Quote: Intracellular mitochondrial presence within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Calcium transient activity within the human heart organoids was assessed using Fluo-4 AM (Thermo Fisher Scientific). Fluo-4 AM was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...
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bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... HCC1395 and SF295 human cancer cell lines were grown in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% FBS and 50 units/ml of penicillin/streptomycin.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: The human promyelocytic leukemia HL-60 cell line (ATCC #CCL-240) was maintained in IMDM media (Gibco) with 10% heat-inactivated (HI ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: Both plasmids encoding for the LC and HC were transfected into Expi293F human cells (Thermo Fisher Scientific) with purified DNA and polyethylenimine (PEI 1:3) ...
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bioRxiv - Cancer Biology 2023Quote: ... SW837 (human colon cells, ATCC CCL-235) were grown in Leibovitz’s L-15 medium (Gibco #11415-049) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
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bioRxiv - Genomics 2022Quote: ... CD4+ T cells were isolated by magnetic negative selection using Untouched Human CD4+ Isolation Kit (Invitrogen 11346D) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
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bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Human cervix adenocarcinoma epithelial HeLa 229 cells (ATCC CCL-2.1) were cultured in RPMI 1640 medium (Gibco) containing 5% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...