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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... The DNA was quantified using a Qubit fluorometer (HS Assay Kit, Life Technologies Inc., Carlsbad, CA, USA) and sheared through Covaris M220 (Covaris ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... The quantity in each prepared library sample was measured using Qubit DNA HS assay kit (Thermofisher #Q32851). The library quality was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent #5067-4626) ...
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bioRxiv - Pathology 2024Quote: Mitochondrial reactive oxygen species were determined by Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, A22188). Prepare 100μl working solution containing 100μM Amplex® Red reagent and 0.2 U/mL HRP (horseradish peroxidase) ...
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bioRxiv - Immunology 2024Quote: ... EdU was detected using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (C10635; Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Total protein amounts were quantified using the Pierce® BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in each sample were then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of cell lysates (30 µg ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using a colorimetric Pierce™ BSA Protein Assay Kit (#23225, Thermo Fisher Scientific). Lysates were subsequently stored at -80°C prior to experimentation.
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bioRxiv - Microbiology 2024Quote: ... RNA was quantiied using the Qubit RNA BR (Broad-Range) Assay Kit on a Qubit Fluorometer (ThermoFisher). The quality of RNA was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... Validation of library construction success was done using the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) to measure concentration and the TapeStation with the High Sensitivity D10000 ScreenTape assay (Agilent ...
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CTCF regulates global chromatin accessibility and transcription during rod photoreceptor developmentbioRxiv - Genomics 2024Quote: ... according to manufacturer’s protocol and was quantified with Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher Scientific). RNA integrity was confirmed with High Sensitivity RNA ScreenTape ...
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bioRxiv - Genomics 2024Quote: ... DNA concentration was verified using the Qubit® dsDNA HS Assay Kit (Life Technologies, Grand Island, NY), and DNA was submitted to the University of Wisconsin-Madison Biotechnology Center ...
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bioRxiv - Genetics 2024Quote: ... ChIP-purified DNA was quantified by fluorimetry using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher), and 0.5–50 ng of DNA/ChIP was used for library preparation using the NEBNext Ultra II DNA Library Kit for Illumina (NEB E7645 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in the supernatants were determined using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by semidry-blotting ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The library was quantified using the Qubit Fluorometer and the Qubit dsDNA HS Assay Kit (ThermoFisher; Q32854). 650 ng of library was sequenced over one PromethION flow cell (ONT ...
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bioRxiv - Molecular Biology 2024Quote: ... reverse transcription was performed using the TaqMan microRNA Reverse Transcription Kit and TaqMan microRNA Assays (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (#23225, ThermoFisher Scientific, Waltham, Ma, USA). Equal amounts of total proteins (10 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... For flow cytometry detection of β-gal the CellEvent Senescence Green Flow Cytometry Assay Kit (C10840, Invitrogen) was used according to the recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured in the cleared lysates using the Pierce BCA Protein Assay Kit (Thermo Fisher). Then ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher, Waltham, MA, USA) and stored at -80°C for future analyses ...
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bioRxiv - Microbiology 2024Quote: ... DNA concentration of the samples was determined using the Qubit dsDNA BR assay kit (Q32850, Invitrogen™) in combination with the Infinite® M1000 PRO plate reader (Tecan) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration from the supernatant was measured using Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat#23225). Samples were then denatured at 950C and resolved on an SDS-PAGE gel ...
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bioRxiv - Neuroscience 2024Quote: ... while library concentration was determined with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). Multiplexed libraries were directly loaded onto a HiSeq2000 (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat#23225).
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bioRxiv - Bioengineering 2024Quote: ... DNA quantitation was performed using a Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes Inc., Eugene, OR) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and the protein concentration determined by a BCA assay (Pierce BCA Kit, Thermo Fisher Scientific). The human protein extracts were further processed to peptides in the same manner as described above for bacterial protein extracts.
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bioRxiv - Biochemistry 2024Quote: ... and inorganic pyrophosphatase (PPase) were added according to the EnzCheck pyrophosphate assay kit (ThermoFisher, Cat # E-6645). The samples were briefly centrifuged for 3 min at 10,000 x g to remove any precipitated Sg MT ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in cell lysates were normalized using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Immunology 2024Quote: ... IL-5 concentration was assessed using mouse-uncoated IL-5 enzyme linked immunosorbent assay (ELISA) kits (Invitrogen) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was established with the BCA Protein Assay kit (#23227, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Cell Biology 2024Quote: ... and quantified on the Qubit 2.0 with the Qubit dsDNA HS Assay Kit (Life Technologies, CA, USA). Per sample ...
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bioRxiv - Neuroscience 2024Quote: ... EdU click-it assay was performed in organoid cryosections per manufacturer’s instructions (EdU kit Invitrogen, #C-10337) followed by immuno-staining for Ki67 ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration and integrity of the RNA were verified using a QubitTM RNA HS Assay Kit (Invitrogen) and denaturing agarose-gel electrophoreses ...
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bioRxiv - Synthetic Biology 2024Quote: ... and quantification was performed using the Qubit™ 1X dsDNA High Sensitivity assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Isolated RNA samples were quantified using both Qubit dsDNA HS (High Sensitivity) assay kit (Invitrogen, catalog# Q32851) and NanoDrop™ 2000/2000c (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were determined using a Pierce™ BCA protein assay kit (Thermofisher Scientific, Cat # 23225). An equal amount of protein was loaded for SDS-PAGE on 10% polyacrylamide gels ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of cell lysates was also determined using a BCA protein assay kit (Thermo Scientific) and data presented depicts caspase-3/7 activity as relative fluorescence unit (RFU ...
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bioRxiv - Microbiology 2024Quote: ... Quality of the extracted DNA was checked using Qubit dsDNA high sensitivity assay kit (Invitrogen, catalog# Q32851) and DNA libraries were prepared using Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Extracted purified DNA was then quantified by Qubit 2.0 with the dsDNA BR assay kit (Thermo Fisher) and normalized to 20 ng/µl in 10 µl volume ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified chromatin and input were quantified using the Qubit dsDNA HS (High Sensitivity) Assay Kit (ThermoFisher Scientific), and equal amounts of DNA were added to each qPCR reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined through the Pierce BCA Protein Assay Kit following the manufacturer’s instructions (Thermo Scientific) and 25 μg of each sample was prepared for SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations from individual samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and luciferase values were normalized to protein.
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bioRxiv - Plant Biology 2024Quote: ... Total protein content was quantified using the Pierce™ 660nm Protein Assay Kit (©Thermo Scientific™). Following quantification ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation of GFP-tagged DSB-1 was performed with the same amount of lysate determined by BCA kit (Pierce BCA protein assay kit #23225, Thermo Scientific, Waltham, Massachusetts, USA) from wild type and pph-4.1 RNAi animals ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were determined using a BCA kit according to the manufacturer’s protocol (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, IL, USA). Citrate synthase (CS ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cleaned with the Qiagen PCR Purification Kit (Valencia, CA) and quantified using a Qubit fluorometer (dsDNA HS Assay Kit, ThermoFisher Scientific, Grand Island, NY). Samples were diluted in 10 mM Tris buffer (pH 8.5 ...
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bioRxiv - Systems Biology 2020Quote: ... Nucleic acid extracts were quantified with the Quant-iT™ HS ds-DNA assay kit and the Quanti-iT™ RNA HS kit with a Qubit™ fluorometer (Invitrogen, USA).
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bioRxiv - Pathology 2021Quote: ... kept on glass slides overnight at 45°C and then in situ hybridization was performed using the Affymetrix ViewRNA ISH Tissue Assay kit (QVT0050) and ViewRNA Chromogenic Signal Amplification kit (QVT0200) (Thermo Fisher Scientific, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and quantified by the Bicinchoninic Acid Assay (BCA) assay (Thermo Fisher). Equivalent masses (15ug ...
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bioRxiv - Neuroscience 2020Quote: ... Actin assay Act5C (assay ID Dm02361909_s1) was acquired from Thermo Fisher Scientific.