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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: siRNA transfections were carried out using 20 pmol of siRNA (SI Table 1) targeting LTBP3 (siLtbp3-1-4) and lipofectamine RNAiMAX (Invitrogen, Waltham, MA; 13778-075) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were probed with respective primary antibodies overnight at 4°C: anti-ZO-1 (1:500, Invitrogen, San Diego, CA, USA, Cat# 61-7300), anti-VE-Cadherin (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... were subjected to overnight incubation at 4°C with a rabbit monoclonal anti-ADRA2A antibody (Thermo Fisher Scientific, Catalog #14266-1-AP, 1:200). Signal amplification was facilitated using the Dako envision + system-HRP labeled polymer anti-rabbit antibody (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa antibody65 (1 : 500 dilution) at 4°C overnight and secondary antibody (Alexa Fluor™ 647 chicken anti-rabbit IgG, Invitrogen; 1 : 500 dilution) at room temperature for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were then incubated overnight at 4 °C in rabbit anti-MYOC (1:50, custom) and rat anti-GFAP (Life Technologies, 130300, 1:500) in 5% NGS (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A, custom antibody from Thermo Fisher 1:1000). Secondary antibody staining was performed using an anti-rabbit antibody (Azure anti-rabbit NIR700 or Azure anti-rabbit NIR800 at 1:20000 dilution) ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Western blots were performed under reducing conditions as previously detailed (6) to probe for PEMT (dilution 1:1000, Thermo Fisher Scientific, PA5-42383) and loading control GAPDH (dilution 1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... On day 6, the medium was changed to neural induction medium (NIM, DMEM/F12 GlutaMAX supplemented with 1:100 N2 supplement (Gibco™, 15502-048), 1 % Non-essential amino acids and 1 µg/ml Heparin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice by resuspending in 1 mL 1% bovine serum albumin (BSA; Bovagen, NZ) in PBS and centrifugation for 1 minute in a mySPIN™6 mini centrifuge (Thermo Scientific, USA). The resulting pellet was permeabilized for 10 min at room temperature in 0.1% Triton®X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Neuroscience 2024Quote: ... and plated again in a dilution 1:6 in neural differentiation medium to induce neuroepithelial differentiation (NDM; 50% DMEM-F12 (Gibco, Grand Island, NY), 50% Neurobasal (Gibco ...
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Developmental Biology 2019Quote: Prab-3::GFP was generated by cutting out Prab-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Developmental Biology 2019Quote: Pelt-3::GFP was generated by cutting out Pelt-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
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bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After the secondary antibody another four 15min washes in PBST (here 0.5% tween) with 1% BSA were performed followed by a short 5min incubation in TO-PRO™-3 Iodide (642/661)(Invitrogen, cat# T3605, 1:1000 dilution). The animals were mounted in moviol with DAPCO on glass slides and stored at 4°C till imaging.
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bioRxiv - Developmental Biology 2019Quote: ... with successfully electroporated embryos fixed using 4% Paraformaldehyde (PFA; Fisher Scientific, 30525-89-4) at 4°C overnight.
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bioRxiv - Physiology 2019Quote: TT’s were visualized by staining with di-4-ANEPPS (4 µmol/l. Invitrogen, UK) as described in detail previously 8,32,70 ...
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bioRxiv - Cell Biology 2022Quote: ... with an Eagle 4 K x 4 K CCD camera system (Ceta CMOS, ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... 4 µg protein was resolved on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher) and transferred to PVDF membrane (Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... containing 2 μM cell-permeant Fluo-4 acetoxymethyl ester (Fluo-4-AM, Thermo Fisher) with the addition of the equal quantity (1:1 v/v ...
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bioRxiv - Immunology 2022Quote: ... Hemocytes were fixed in 4% PFA (4% in PBS; J19943-K2, Thermo Fisher Scientific) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 4% formaldehyde (4% FA, 10% formalin [Thermofisher Scientific, #119690010] in 1x PBS), dissected out the spinal column ...
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bioRxiv - Genetics 2021Quote: ... The cells were stained overnight at 4 °C with a 1:50 dilution of phalloidin-Alexa 546 (Invitrogen) made in blocking buffer followed by three washes each of 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Ovaries were washed 4×20min in PBT and then incubated in PBT containing DAPI (1:1000, Molecular Probes), with or without DiOC6 (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Boiled samples were loaded into 4-12% 1-mm 12-well Bis-Tris NuPage gels (Invitrogen Cat#NP0322PK2) along with 0.5-μL of Benchmark Unstained Protein Ladder (Invitrogen Cat#10747-012 ...
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bioRxiv - Genomics 2020Quote: ... were washed in IP-buffer and incubated with 4 μg anti-AP-1 (Thermo Scientific™ #MA5-15172)or anti-Sp1 Ab (ChIPAb+™ Merck #17-601 ...