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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated cRNA libraries were synthesized from 250 ng of total RNA using the Affymetrix HT 3’ IVT Plus kit (Affymetrix, Santa Clara, CA) and the Beckman Biomek FXp Laboratory Automation Workstation (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135; 10 kD BDA Invitrogen D1956; diluted in 0.9% NaCl) were used as retrograde and anterograde tracers ...
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bioRxiv - Genomics 2022Quote: ... The precleared nuclear lysates (2 mg) were incubated with purified biotinylated RNA in NT2 buffer along with Yeast tRNA (20 µg/ml; Thermo Fisher Scientific #AM7119) with gentle rotation for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... From each sample 250ng of total RNA were used to generate amplified and biotinylated sense-strand cDNA according to the GeneChip WT PLUS Reagent Kit User manual (Affymetrix, Santa Clara, CA). GeneChip Human Gene 2.0 ST Arrays were hybridized for 16h in 45°C incubator ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
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bioRxiv - Molecular Biology 2020Quote: ... affinity columns were prepared by immobilizing the biotinylated antibody (2.5 μg of anti-Apo CIII) with streptavidin magnetic beads (Dynabeads, Life Technologies, Grand Island, NY). Following sample preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated cRNA libraries were synthesized from 500 ng of total RNA using the Affymetrix HT 3’ IVT Express kit (Affymetrix, Santa Clara, CA) and ta Beckman Biomek FXp Laboratory Automation Workstation (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100ng of total RNA was used for biotinylated cDNA synthesis using GeneChip™ WT Plus Reagent Kit (Invitrogen, 902281, lot no. 01059768) as per manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... IgGs were randomly biotinylated at primary amines using EZ-link NHS-PEG4 Biotinylation Kit according to the manufacturer’s protocol (Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 RBD (2.5 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: Pull-down experiments to investigate the effects of Nanoglue and Nanobreak on the Unc5 – GPC3 interaction were performed by coupling biotinylated hGPC3core to high-capacity streptavidin agarose resin (Thermo Fisher Scientific, 20357). Unc5ecto was mixed with nanobody in 1:1 ...
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bioRxiv - Neuroscience 2022Quote: Wild-type mice received a single iontophoretic injection of each anterograde tracer: biotinylated dextran amine (BDA; 10 kDa; 5% solution in 0.125 M phosphate buffer, pH 7.4; Thermo Fisher Scientific, Cat. No. D1956) or Phaseolus vulgaris leucoagglutinin (PHA-L ...
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bioRxiv - Cell Biology 2023Quote: ... The tetramers were produced by incubating biotinylated H-2Db-NP396-PE (FQPQNGQFI) monomers (from NIH Tetramer Core Facility) with streptavidin-PE (Thermo Fisher Scientific, S866) at a molar ratio of 3:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Nascent RNA was labeled with biotinylated azide and captured on magnetic beads using the Click-iT Nascent RNA Capture Kit (Molecular Probes, cat # C10365). cDNA was synthesized from RNA bound to the beads using the SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Human LDL-c and HDL-c were biotinylated using Sulfo-NHS-LC-biotin and purified using a 10Kd-cut off desalting column (ThermoFisher, Waltham, MA, USA). The biotin-LDL-c (2 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then depleted of 2S rRNA by Oligo Capture using biotinylated oligo d2227 and magnetic Dynabeads MyOne Streptavidin C1 (Life Technologies cat. #65001) following the protocol described by [15] ...
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bioRxiv - Neuroscience 2023Quote: ... then sections were blocked with 3% milk in 0.25% TBS-X (Triton X-100) for 0.5 h followed by incubation at 4°C overnight with biotinylated AT8 antibody (Thermo Scientific, MN1020B, 1:500). The next day ...
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bioRxiv - Systems Biology 2020Quote: ... An Ion Torrent PGM was used with the Hi-Q 400 template preparation and sequencing kit and a 318v2 sequencing chip (Thermo Fisher, MA). All the quality control steps were performed using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: The pFastBacNKI-his-3C-m6A-Tracer construct was used for creation of baculovirus according to the Bac-to-Bac system (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... of 8 mice were then resuspended in 10 ml DMEM supplemented with 10 % heat inactivated new born calf serum (HI NCBS, LifeTechnologies / Gibco, # 26010-074) and plated on two 10 cm dishes (Corning ...
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bioRxiv - Genetics 2021Quote: One microliter of GlobalFiler™ or GlobalFiler™ Express amplified product was added to 9.5 μL Hi-Di™ formamide (ThermoFisher Scientific) and 0.5 μL GeneScan™ 600 LIZ® size standard (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the SBT3.8 ORF was amplified from genomic DNA with a reverse primer including six His codons and first cloned into pCR2.1-Topo (Life Technologies, Carlsbad, CA). EcoRI sites from pCR2.1 were used for ligation into pART7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were then pooled and loaded onto chips and sequenced on Ion Torrent PGM using Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific). Fast-q or BAM files from sequencing runs were analysed in Geneious ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Genetics 2019Quote: ... using 1 μl PCR product in 9.5 μl Hi-Di™ formamide containing 0.5 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: The Taq polymerase-amplified PCR product of the rdLRP was inserted into the TOPO Cloning site of a pcDNA3.1/V5-His-TOPO vector (Thermo Fisher Scientific, MA). The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The full-length OpIE2 promoter was obtained from the pIB/V5-His by PCR using Phusion High fidelity DNA Taq polymerase (ThermoFisher Scientific, USA) according to the following program ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 μl of fivefold-diluted PCR product was mixed with 9.25 μl of Hi-Di formamide and 0.25 μl LIZ600 size standard (ThermoFisher Scientific, Waltham, MA, USA), denatured for 10 min at 95°C and then immediately cooled on ice ...
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bioRxiv - Cell Biology 2019Quote: ... and FLAG-dDYNLL1 (EcoRI/XbaI) fragments were inserted into the S2-cell expression vector pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020).
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μl PCR product in 9.6 μl Hi-Di™ formamide containing 0.4 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were run on a 15% PAGE and developed using monoclonal anti-His antibody (Biospecs, BTL1010) with super signal west femto maximum sensitivity substrate (Thermo Scientific, 34095).
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Genetics 2020Quote: The PCR products were diluted 1:1 with milliQ water and 1μL of the diluted PCR product was added to a 384-format genotyping plate containing 4μL of a mix of Hi-Di™ formamide (Applied Biosystems™) and Genescan™ 600 LIZ™ dye size standard v2.0 (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...