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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mandibular alveolar bone tissue was cut into small pieces (<1 mm3) and digested with 3 mg/mL collagenase type I (Gibco) and 4 g/mL dispase II (Sigma ...
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bioRxiv - Immunology 2022Quote: The EV-enriched fractions (1-3) were combined and concentrated using Amicon Ultra 0.5mL 30kDa cutoff spin columns (Cat. No. 10012584, Fisher Scientific). 300 mesh continuous carbon support copper grids (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... HEK-293T cells were transfected with plasmids encoding the WNV replicon and ZIKV structural genes at a 1:3 ratio by mass using Lipofectamine 3000 (Invitrogen), followed by incubation at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cortical astrocytes were dissected from SD neonatal rats (1-3 days) with the aseptic operation and digested by 0.25% trypsin (Gibco, USA) at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times and incubated with 1/1000 of monoclonal secondary antibody conjugated to Alexa Fluor 594 (Invitrogen) for 45 min at RT in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... These prepared samples were loaded onto 3–12% Bis-Tris Native PAGE gels and electrophoresed using a 1× native PAGE running buffer system (Invitrogen). The cathode buffer included 1× cathode buffer additive (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... The following day the sections were washed 3 times for 5 min each in PBS-T and incubated with the corresponding secondary antibodies (all 1:1000, Invitrogen), for 2 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed with 1% BSA in PBS (180 x g, 3 minutes) before adding viability dye per manufacturer’s instructions (Invitrogen, #L34989). Antibody binding capacity of cells was assessed using 1 µg/mL DUNP19 and anti-human IgG Simply Cellular bead standards (Bangs Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at RT and incubated with 3% NGS PBS containing secondary antibodies (1:600, Alexa-Fluor 594 anti-mouse IgG, Invitrogen and Alexa-Fluor 488 anti-guinea pig IgG ...
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bioRxiv - Neuroscience 2024Quote: HAP1 WT and SNCA KO cells were seeded for ∼75% confluency and transfected with NHEJ and HR plasmid reporters51 using X-tremeGENE HP DNA Transfection Reagent (1:3 DNA:reagent) in OptiMEM (Gibco #31985062). Images were taken on a Zeiss Axio Observer.D1 outfitted with an Excelitas X-Cite 120 LED GFP light at 24 ...
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bioRxiv - Biochemistry 2024Quote: ... cartilage shavings were submerged in 3:1 methanol:water and homogenized using a tissue homogenizer (SPEX Sample Prep 1200 GenoLyte, Fisher Scientific). Homogenization included 15 cycles of homogenizing for 20 seconds and resting for 2 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-treated cells were washed twice with 37°C M1 and replaced with M1 containing 1 μM TO-PRO-3 iodide (ThermoFisher) or washed once with 37°C M2 containing 5 mM ethylene glycol-bis(2-aminoethylether)-N ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µL of 1 mM TO-PRO-3 Iodide in DMSO (Thermo) was added to stain dead cells and 1uL of DRAQ5 (Invitrogen) was added to stain viable cells ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Developmental Biology 2024Quote: ... 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’) and 1 μl of 10 mM dNTP mix (Thermo Fisher). Illumina libraries were prepared by using a modified smart-seq2 protocol [Picelli 2014] using SuperScript IV RT and tagmentation procedure as previously described [Henning 2018] ...
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bioRxiv - Genomics 2024Quote: ... and nuclei were washed once with nuclei suspension buffer (NSB; 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% Superase RNA Inhibitor (Thermo Fisher), 1% 0.2mg/mL Ultrapure BSA (New England Biosciences)) ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected using Alexa Fluor 488 labeled goat anti-rabbit IgG antibodies diluted 1:1000 in PBS containing 3% BSA (Invitrogen). Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were denatured by adding 3× SDS sample buffer (containing 188 mM Tris-HCl, pH 6.8; 3% SDS; 30% glycerol; 0.01% bromophenol blue; and 1× Bond-Breaker TCEP solution from Thermo Fisher Scientific) and heating at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... mouse embryonic fibroblasts 3T3-J2 cells (kind donation from Howard Green, Harvard Medical School, Boston52) with keratinocyte culture medium cFAD (3:1 DMEM (Gibco) to Ham F-12 Nut Mix (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... and 100 µl of the homogenate were added to 1:3 v/v of TRIzol™ LS (Thermo Fisher Scientific) and incubated for 10 min at room temperature to inactivate the virus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twenty-four hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HA-Gα-LgBiT constructs and SmB-cmyc-PDZ-RhoGEF were co-transfected using a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). After 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3 times with 5% milk TBST and incubated with HRP-conjugated secondary antibodies (1:5000, 32230/32260; Invitrogen). Data were visualized using chemiluminescence detection on ChemiDoc Touch (Bio-Rad Laboratories).
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... and chromosome III probes (3 BACS covering approximately 1% of the chromosome) were labeled with AlexaFluor 488 (Molecular Probes, F32947).
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bioRxiv - Immunology 2023Quote: ... 1×106 primary human keratinocytes were seeded into the inserts and kept submerged in a 3:1 DMEM (Hyclone, #SH30243.01) and F12 (Gibco, #31765035) mixture with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the indicated plasmids routinely using 1 μg of DNA mixed with 3 μl of Lipofectamine 2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Developmental Biology 2023Quote: ... Stained samples were washed (3 x 5 minutes) and incubated in DAPI solution (Invitrogen, D3571; diluted 1:1000 in DPBS) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed three times for 3 min in PBST and incubated in an anti-rabbit AF488 (1:2000, Invitrogen) and DAPI (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... 1µg of DNA and 3µl of FuGENE® (1:3 ratio) were combined with 100µl of Opti-MEM™ (Gibco), vortexed and incubated at room temperature for 30 minutes before adding to the cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...