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Citations for Thermo Fisher :
5551 - 5600 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... DRG expression levels were determined from total RNA extracts measured using Affymetrix’ Human Transcriptome Array 2.0 (Affymetrix, Santa Clara), scanned with Affymetrix’ GeneChip 3000 G7 Instrument System ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Genetics 2023Quote: ... 500 ng of PCR products were saturated with 2 μg of unlabeled Cot1 human DNA (Invitrogen, cat. 15279-011) and 0.2 nmol of oligonucleotides corresponding to the universal Illumina adaptors (see above ...
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Microbiology 2023Quote: The adult human dermal fibroblast cell line (HDFα, FH1092, FuHeng Biology) was cultured in RPMI 1640 medium (11875093, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: The gene sequences of human MCHR1(residues 70-405) and MCHR2(residues 1-331, with L2566.40Y mutation) was subcloned into pFastBac vector (Invitrogen) with an N-terminal haemagglutinin signal peptide (HA ...
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bioRxiv - Microbiology 2023Quote: All parasite lines were maintained in human foreskin fibroblasts (HFFs; ATCC) in Minimal Essential Media-α (MEM-α; Gibco) supplemented with 7% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Cancer Biology 2023Quote: ... murine or human (Panc-1) PDAC cells were stained in suspension with CPD670 (#65-0840-85, Thermo Fisher Scientific). After labeling ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Biochemistry 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a human IL-8 ELISA kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Microbiology 2023Quote: Mouse fecal pellet and human stool sample data analysis was performed using FreeStyle software (version 1.8 SP2, Thermo Scientific). Metabolite identification was established by matching accurate mass ...
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...
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bioRxiv - Microbiology 2023Quote: The human-induced pluripotent cells (hiPSCs) used in this study were reprogrammed from adult dermal fibroblasts (HDFa, Gibco, C0135C) using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled RNA were hybridized to Affymetrix Gene Chip Human Gene 1.0 ST arrays (Thermo Fisher Scientific, Meerbusch, Germany, 901086). cDNA syntheses ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... CFSE-stained T cells were resuspended at 1e6/ml and DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) were added at a ratio of 1:10 beads:cells ...
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bioRxiv - Genomics 2024Quote: HEK 293T and human foreskin fibroblasts (HFF) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific), supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Immunology 2024Quote: ... The following fluorochrome-labeled anti-human Abs were used for surface staining: PerCP/Cy5.5-CD19 (Invitrogen, #45-0198-42), APC-CD27 (eBioscience ...
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Interaction between gene expression and morphokinetic parameters in undisturbed human embryo culturebioRxiv - Developmental Biology 2024Quote: ... Labelled PolyAcDNA was then hybridised to the GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix, SantaClara, CA, USA) that covers over 47,000 transcripts and have been commonly used for human transcriptome analysis ...
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bioRxiv - Developmental Biology 2024Quote: Human Umbilical Vein Endothelial Cells (HUVEC) (ATCC, Cat. #: PCS100010) were culture in 1X M-199 Medium (Gibco, Cat. #: 12340030) supplemented with 16 % FBS (Gibco ...
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bioRxiv - Genomics 2024Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Neuroscience 2024Quote: ... Human iPSCs were cultured as colonies in feeder-free conditions using Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES-qualified (Corning ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... primary human CD4 T cells were activated with anti-CD3+ anti-CD28 Dynabeads (1:1) (Thermofisher scientific, cat # 11161D) + IL-2 overnight and then 1 million aliquots of cells (triplicates ...
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bioRxiv - Cell Biology 2024Quote: RNA extracted from human left ventricular tissue was digested with DNase I (Invitrogen DNA-free DNA removal kit AM1906). 20 ng digested RNA was depleted of rRNA (NEBNext rRNA depletion kit E6350L) ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into human embryonic kidney (HEK)293 T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudo-typed viruses of SARS-CoV-2 variants were generated using the Spike protein from WA1/2020 (Wuhan/WIV04/2019 ...
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bioRxiv - Microbiology 2024Quote: T24 human epithelial bladder cells (ATCC HTB-4) were grown in McCoy’s 5A modified medium (Gibco 16-600-082) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... activated with anti-CD3/anti-CD28 activation beads per manufacturer’s instructions (Gibco Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation, Thermofisher), stimulated with IL-2 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from human samples using Ficoll-Paque density centrifugation (Corning, Fisher Scientific, CA). PBMC were cultured in RPMI 1640 medium (Corning ...
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bioRxiv - Microbiology 2024Quote: Promutagens were first activated by incubating in equal volumes with a 2-fold human microsomal (ThermoFisher Cat. No. HMMCPL) reaction mixture containing 1mg/mL microsomes ...
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bioRxiv - Microbiology 2024Quote: The TR146 human buccal epithelial squamous cell carcinoma cells (MilliporeSigma) 50 were incubated in DMEM/F12 medium (Gibco, #11320033) with 10% fetal bovine serum and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (32)) cloned into pCEP4 (Invitrogen) unless indicated otherwise ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Physiology 2024Quote: ... The PCR amplification of human Piezo1 was performed using a Veriti Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) and ExTaq (Takara ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fragments of human L1 ORF1 and ORF2 from the EF06R plasmid were amplified by PCR and cloned into pcDNA3.1(+) (#V79020, Invitrogen) with the puromycin resistant gene split by an intron ...
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bioRxiv - Molecular Biology 2024Quote: Quantitation of human DNA isolated from blood cells was performed using Qubit 2.0 with the dsDNA High Sensitivity Assay Kit (Thermofisher A custom Ampliseq panel for the detection of SNPs was designed using Ion AmpliSeq Designer and ordered as Ampliseq Custom Panel ...
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bioRxiv - Microbiology 2024Quote: ... and human embryonic kidney-293T (HEK-293T) cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco), with 10% heat-inactivated FBS (Millipore Sigma) ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...