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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies used in this study were listed in Table S1 and secondary antibodies fused to Alexa fluorophore (Molecular Probes, 1:500). All stained sections were analysed with Zeis Imager Z2 (Zeis ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Genomics 2019Quote: ... Membrane was probed using GATA1 antibody (1:1000, rabbit, Cell Signalling Technologies cat no. 3535S) and GAPDH antibody (1:2000, mouse, ThermoFisher cat no. AM4300) as primary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Vglut2a:DsRed] larval brains were stained using activated caspase-3 antibody followed by anti-rabbit IgG Cy5 conjugated antibody (Molecular Probes, A-10523,1:300) and imaged as previously described ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then incubated overnight at 4 °C in 1:100 (in antibody diluent) anti-cadherin-11 antibody (CDH-11, rabbit polyclonal, Thermo Fisher, 71-7600). Cells were washed three times with PBS-tween and incubated in the conjugated secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated and unconjugated primary antibodies were detected by incubating with Streptavidin-PE (Pharmingen, 534061) and Rat anti-mouse IgG2a antibody (Invitrogen, 17-4210-80) respectively ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... then Western blots were performed using the anti-GFP antibody (1:2500) or a mouse anti-TfR1 antibody (ThermoFisher #13-6800, 1:500) or a secondary anti-rabbit HRP (Promega #W4011 ...
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bioRxiv - Cell Biology 2019Quote: ... and then specific antibodies were added overnight at 4°C prior to 30 min of secondary antibody (AlexaFluor488 conjugated; Molecular Probes, 1:1000) and DAPI staining ...
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bioRxiv - Biochemistry 2019Quote: ... Sections were washed 3x in 1x PBS and bound primary antibodies were detected by incubation with Alexa-fluor conjugated secondary antibodies diluted in blocking buffer (Thermo Fisher/Invitrogen, Eugene, OR) for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed in PBS and incubated with the appropriate AlexaFluor-564 conjugated antibody and AlexaFluor-488 conjugated secondary antibody (Molecular Probes; 1:200) as well as with Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody, cat no. PA5-17511, Thermo Fisher Scientific, Rockford, USA) and loading control protein ATP5A antibody (1:1.000 anti-ATP5A antibody ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibody incubation was carried out using a 1:1000 dilution of Alexa 647 conjugated donkey anti-goat antibody (Life Technologies, Carlsbad, CA) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with primary antibodies (anti-phos-CD31, anti-CD31, and anti-mCRP) and subsequently stained with secondary antibodies (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated with ACE2 and TTF-1 primary antibodies and the binding of the primary antibodies to their respective antigenic substrates was revealed by made-specific secondary antibodies conjugated with Alexa-488 (Life Technologies, 1:250) and Alexa-568 (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated sequentially with primary antibodies that detect either FLAG or phospho-Histone H2A.X (Ser139) and secondary antibodies (Alexa 488, Molecular Probes, Eugene, OR, US). Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’ ...
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bioRxiv - Immunology 2021Quote: ... The binding of the primary antibodies to their respective antigenic substrates was revealed by made-specific secondary antibodies conjugated with Alexa-488 (Life Technologies, 1:250) and Alexa-568 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... including an incubation in the donkey anti-goat Alexa 488-conjugated secondary antibody as indicated above and then in a donkey anti-rabbit Alexa 546-conjugated secondary antibody (A10040, 1:500; Life Technologies, Madison, WI) overnight at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Neuroscience 2022Quote: Microglia activation was also assessed using the rabbit polyclonal Anti-Iba1 antibody (Wako Chemicals; 1:800) and the secondary antibody Donkey Anti-rabbit IgG conjugated with Alexa-594 (Thermo Fisher; 1:500), as previously described ...
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bioRxiv - Microbiology 2023Quote: ... the parasite mitochondrion was visualized using a polyclonal Rabbit anti-HSP60 antibody (Novus NBP2-12734) and AlexaFluor 647-conjugated Goat anti-Rabbit 2° antibody (Invitrogen Life Technologies A21244), the nucleus was stained with ProLong Gold Antifade Mountant with DAPI (Invitrogen Life Technologies P36931) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS three times and incubated with antibody solution with Alexa 568-conjugated anti-mouse IgG antibody (Molecular Probes, A-11019) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... bound antibody was detected by incubation for 1 h with secondary antibodies conjugated with Alexa Fluor 488 (1:200, Thermo Fisher Scientific, USA) or Alexa Fluor 546 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: Secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Alexa Fluor 488, Thermo Fisher Scientific, A-21202, 1:1000), Donkey anti-Mouse IgG (H+L ...
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Laser Scanning Confocal printing, a new simple method for micro-device fabrication (LSCprint method)bioRxiv - Bioengineering 2024Quote: ... After washing, incubation 30’ with secondary antibodies and DAPI (Termofisher, D1306) was performed: an Alexa 488 anti-mouse antibody (Thermo Fisher A-21202) was used to stain PH3-Ser 10 ...
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bioRxiv - Biochemistry 2024Quote: ... using a rabbit anti-WCTD antibody (GeneScript) (1:100) and Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary antibody (Invitrogen, Cat# A-31573) (1:500) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1.5 hours at room temperature with goat anti-rabbit HRP-conjugated antibody or donkey anti-chick HRP-conjugated antibody (Thermo Fisher Scientific, Massachusetts). Protein expression was detected by chemiluminescence using ECL (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were incubated first with anti-Ybx1 primary antibody and then Alexa Fluor 488/568-conjugated secondary antibody (Life Technologies, Carlsbad, CA). The sections were counterstained for nuclei with Hoecyst33342 ((Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed several times with PBS and subsequently incubated with secondary antibody with the following antibodies in blocking solution for 30-60 minutes: donkey anti-rabbit Alexa Fluor 488 (Themo Fisher Scientific; A-21206), donkey anti-mouse Alexa Fluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
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bioRxiv - Cancer Biology 2023Quote: ... Next day sections were washed with 10% normal goat serum and incubated with appropriate secondary antibodies for 1 hour at room temperature in a humidified chamber and with a secondary antibody cocktail (Life Technologies, Waltham, MA) of Alexa Fluor 546 (1:250) ...
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bioRxiv - Molecular Biology 2023Quote: ... BMP6 was a generous gift from OSTEOGROW. ERFE was detected using DYKDDDDK Epitope Tag HRP-conjugated Antibody (R&D, Cat. # HAM85291) and FAM132B Polyclonal Antibody (Invitrogen, Cat. #PA5-67448). Recombinant ALK3-Fc fusion protein was purchased from R&D (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:200) followed by application of goat anti-rabbit 568 secondary antibody (Invitrogen, #A11011, 1:400). Samples were additionally labeled with Phalloidin for visualization of actin filaments (Alexa Fluor 488 ...
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bioRxiv - Microbiology 2023Quote: ... followed by the incubation with secondary antibody (HRP-conjugated goat anti-mouse antibody (1: 10,000; NA931V, Cytiva) or HRP-conjugated goat anti-rabbit antibody (1: 10,000; A-11036, Thermo Fisher Scientific, Waltham, MA, USA). The immunoreactive bands were visualized using Chemi-Lumi One Super Kit (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: ... and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631, 1:500 in TBST). Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380 ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were used with goat anti-Rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo fisher, SA5-10010, 1:400 dilution) or goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Bioengineering 2023Quote: ... human vimentin antibody was detected with a HRP-conjugated horse anti-rabbit antibody and diaminobenzidine (DAB) to produce a brown color (Thermo Fisher Scientific, US). All sections were counterstained with Mayer’s hematoxylin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS and incubated in secondary antibody mixtures containing either donkey anti-rabbit antibody (Invitrogen #A10042, 1:500 dilution) or donkey anti-goat antibody (Invitrogen #A-11057 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with IF buffer and incubated with the following secondary antibody: Goat anti-Rabbit IgG secondary antibody (Alexa 568, Invitrogen-Molecular Probes : A-11011) for 1 hour at room temperature in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies used for immunofluorescence were Alexa Fluor™ 488 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (ThermoFisher # A-11029) and Alexa Fluor™ 568 Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with the following secondary antibodies diluted 1/200 : Goat anti-Mouse IgG secondary antibody (Alexa 488, Invitrogen-Molecular Probes : A-A11001), Goat anti-Mouse IgG secondary antibody (Alexa 568 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody membranes were incubated in the appropriate HRP-conjugated secondary antibody (either mouse, Thermo Scientific #31430, or rabbit, Thermo Scientific #31460) for two hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary antibodies were incubated overnight at 4°C before a 2-h incubation with secondary antibodies (1:500, Invitrogen, #A21428 and #A21235) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 568 and 647 secondary antibodies against mouse and rabbit IgG were used to detect the forementioned primary antibodies (Molecular Probes and Jackson Immunoresearch) The secondary antibodies were applied 2 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slices were then incubated with appropriate secondary antibody: goat anti-mouse Alexa Fluor 594 secondary antibody (1:500, cat. # A21125, Thermo Fisher Scientific [RRID:AB_141593]), goat anti-rabbit Alexa Fluor 647 secondary (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... gingivalis-specific antibody (1:1000) and a goat anti-rabbit Alexa fluor 488-conjugated secondary antibody (1∶2000, Invitrogen, Carlsbad, CA; Cat. # A27034) was used to stain P ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 568 donkey anti-rabbit antibody (Invitrogen, A11011, 1:500, and Alexa Fluor 488 goat anti-rabbit antibody (Invitrogen, A11008, 1:500) in PBS for 1h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then rinsed with 1X PBS and incubated with Alexa Fluor-conjugated secondary antibodies: 488 Phalloidin and 594 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific, 1:1000). All samples were counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were then transferred to PVDF membranes at 100 V for 2 hours and blotted with the indicated primary antibodies (Table 4. Secondary anti-mouse HRP antibody and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific 34096) was used to visualize bands with a Bio-Rad ChemiDoc Imaging System.