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Citations for Thermo Fisher :
5551 - 5600 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Amplified 16S rDNA was obtained from each strain by polymerase chain reaction (PCR) with the universal primers in a Thermal cycler (Applied Biosystems-2720, USA). Sequencing of the amplicons was performed in an automated DNA Analyzer (ABI 3730XL Capillary Sequencers ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment that contains IVS-Syn21-GCaMP6f (codon optimized)-p10 elements was amplified from the genomic DNA of the transgenic strain that carries 13XLexAop2-IVS-Syn21-GCaMP6f-p10 (in su(Hw)attP1) by PCR (Phusion Green, ThermoFisher Scientific cat# F534). This fragment was subcloned into pCR Blunt II TOPO vector using the Zero Blunt TOPO kit (ThermoFisher Scientific cat# K287540) ...
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bioRxiv - Plant Biology 2022Quote: ... Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific, California, USA). Fermentation was conducted in approximately 3 L of culture in a bioreactor BioFlo120 (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Biochemistry 2020Quote: ... strains were negatively selected against the URA3 marker gene using 1 mg/mL of 5-Fluoroorotic Acid (5-FOA) (Fisher Scientific, F10501-5.0) in SD-plates ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from trophozoites of EhPTEN1 gene silenced and control strains that were cultivated in the logarithmic phase using TRIZOL reagent (Life Technologies, California, USA). Approximately one µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... pseudotuberculosis strains carrying complementation constructs (pGM930) and pMK-Bla (YopE-TEM, [87] using LiveBLAzer-FRET B/G Loading Kit (Life Technologies, Carlsbad, USA). Strains were pregrown in LB containing 0.1 % (w/v ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Physiology 2021Quote: ... Macrophages were incubated with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) in phenol red-free ...
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bioRxiv - Molecular Biology 2021Quote: ... were determined by matching the protein database (Paramecium tetraurelia strain d4-2 MAC genome (v1.0)) with the acquired fragmentation pattern by the software program Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Microbiology 2022Quote: ... All Escherichia coli strains were grown in Lysogeny broth (LB) at 37°C in a shaking incubator (Thermo Fisher Scientific, Waltham, MA, USA) at 250 rpm ...
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bioRxiv - Immunology 2022Quote: ... bovis BCG (Pasteur strain) was grown in Middlebrook 7H9 broth supplemented with Middlebrook oleic acid-albumin-dextrose-catalase (OADC) enrichment (Invitrogen Life Technologies, Carlsbad, CA), 0.002% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was extracted from trophozoites of gene silenced and control strains that were cultivated in the logarithmic phase using TRIZOL reagent (Life Technologies, California, USA). Approximately 1 µg of DNase treated total RNA was used for cDNA synthesis using Superscript III First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... strain CNRVC190243 was cultured in Brain-Heart-Infusion (BHI) broth (Difco) overnight at 37 °C with shaking (200 rpm—Thermo Scientific MaxQ 6800). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain) on the automated KingFisher Flex platform using the MagMax™ Viral/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific, UK). Post extraction DNase treatment was performed using the TURBO DNA-free™ Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... from each strain was amplified with random primers to synthesize cDNA with the High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Carlsbad, CA). Primer pairs listed in Table S1 were used to amplify cDNA to measure expression of the S ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant pPICZαA plasmids containing the WT and mutant THCAS were used to transform the Komagataella phaffii strain X-33 (Invitrogen™, Thermo Fisher Scientific), as specified by the supplier ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed 3x in PBS and then stained for 20 min at RT with 2 drops/mL of NucBlue Fixed Cell Strain ReadyProbes (Invitrogen, Carlsbad, CA, USA) and ActinGreen 488 Ready Probes (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... low glucose DMEM with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant pPICZαA plasmids containing the WT and mutant THCAS were used to transform the Komagataella phaffii strain X-33 (Invitrogen™, Thermo Fisher Scientific), as specified by the supplier ...
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bioRxiv - Plant Biology 2024Quote: 10 µg of plasmid DNA linearized using SpeI was transformed into electrocompetent PichiaPink™ strain 4 (ThermoFisher; P. pastoris genotype ade2, pep4, prb1) targeted to the TRP2 locus ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... Target DNA fragments were amplified from gDNA of clinical strains DK12 Late and DK36 Late using the Phusion U polymerase kit (Thermo Fisher Scientific, USA). For the ΔpscC mutant strain primers were designed to create matching short overlaps between the DNA fragments that should be stitched together in the final plasmid ...
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bioRxiv - Genomics 2024Quote: ... the HP1-2xFKBP-GFP-2A-NeoR / mCherry-FRB-miniTurbo and BDP5-2xFKBP-GFP-2A-NeoR / mCherry-FRB-miniTurbo strains were cultured in both the presence of Gentamicin G-418 sulfate (400 ug/mL, Invitrogen, ant-gn-5) and Blasticidin (0.4 ug/mL ...
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bioRxiv - Microbiology 2024Quote: ... target DNA fragments were amplified from the respective clinical strain genome with the corresponding primers using Phusion U polymerase kit (Thermo Fisher Scientific, USA), cloned into the pACRISPR plasmid using the USER® Enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and goat anti-mouse IgG (H+L) Alexa Fluor 647 (1:500; Invitrogen) with Hoechst 33342 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... with high glucose and L-glutamine (11965-167) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-rabbit IgG (H+L) coupled to AlexaFluor594 (1/400; Thermo Fisher Scientific Cat# A-21207 ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 568 goat anti-mouse IgG (H+L) (A-11004, Invitrogen). The nuclei were stained with DAPI (D9542 ...
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bioRxiv - Immunology 2021Quote: ... 647-labelled goat anti-rabbit IgG (H+L) (1:500, ThermoFisher, A21244, RRID:AB_2535812). After incubation with secondary antibodies at room temperature for 1 h ...
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bioRxiv - Immunology 2021Quote: ... HRP conjugated Rabbit anti-Human IgG(H+L) (Cat. No. A18903, Invitrogen, USA) as added to each well as the detection antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... Alexa Fluor 488 or 594 IgG (H+L) antibody (Thermo Fisher, Massachusetts, USA) and horseradish peroxidase-coupled IgG antibody (Santa Cruz ...
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bioRxiv - Molecular Biology 2021Quote: ... ± dox with Leibovitz’s L-15 phenol red-free (CO2 independent media; #21083027 Thermofisher) supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... and Alexa Fluor 647 goat anti-rabbit IgG(H+L) (1:1000; ThermoFisher). Embryos were mounted in glass-bottomed Petri dishes for imaging as described above.
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bioRxiv - Cancer Biology 2022Quote: ... and anti-Rabbit IgG (H+L) Alexa Fluor 647 (goat, Thermo Fisher Scientific) secondary antibody ...
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bioRxiv - Biophysics 2022Quote: ... Injected oocytes were incubated in OR3 solution (Leibovitz’s L-15 medium (Life Technologies) with 5 mM L-glutamine ...
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bioRxiv - Genetics 2022Quote: ... All suspension cells were cultured in RPMI-1640 with L-glutamine (Life Technologies), supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) (ThermoFisher Scientific, A11001) and Alexa Fluor 647-conjugated goat anti-mouse IgG (H + L ...
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bioRxiv - Molecular Biology 2021Quote: ... except with no serum replacement and replacing L-glutamine with GlutaMAX (Thermofisher, 35050038), as were EpopGT/GT and EpopWT/WT cell lines (gifts from Luciano Di Croce ...
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bioRxiv - Cell Biology 2022Quote: ... For live cell imaging experiments cells were imaged in Leibovitz L-15 (Thermofisher) medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... goat α-rabbit IgG (H+L) F(ab’)-Alexa Fluor 488 (Invitrogen A11070) diluted in Co-detection Antibody Diluent 1 hour ...
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bioRxiv - Genomics 2020Quote: ... supplemented with L-glutamine (2mM) and penicillin/streptomycin (all reagents from Life Technologies) at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Alexa Fluor 568 goat anti-rabbit IgG (H+L) (Invitrogen A21069, 1:500), Goat anti-mouse IgG (Cy3 (Abcam ab97035 ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% heat-inactivated autologous plasma and 2 mM L-glutamine (Life Technologies), unless otherwise stated ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM L-Glutamine and 0.1 mM non-essential amino acids (ThermoFisher Scientific).
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bioRxiv - Physiology 2019Quote: ... centrifuged at 200 g and resuspended in growth medium (L-15, Life Technologies) adjusted to 280-290 mOsm with mannitol and to pH 7.75 with 10 mM NaHCO3 ...
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bioRxiv - Neuroscience 2019Quote: ... Immunoprecipitation (IP) of transcripts was achieved via biotinylated-protein L (ThermoFisher Scientific 29997)-coated streptavidin magnetic beads (ThermoFisher Scientific 65602 ...
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bioRxiv - Developmental Biology 2020Quote: ... Neurobasal-A medium was supplemented with l-glutamine (Gibco; cat. no. 25030-123), B27 (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue slices were then submerged in ice-cold Leibovitz’s L-15 medium (Gibco) in a petri dish and the striatum was dissected out and stored in 1 ml ice-cold Leibovitz’s L-15 medium containing 1μl SUPERase RNase inhibitor (20 U/μl ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were washed once then incubated in Leibovitz’s L-15 medium (Gibco) and imaged at 37°C in a temperature-controlled and humidified live-imaging chamber (Tokai Hit ...