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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The bones were broken into fragments and incubated for 1 hour at 37 °C in phosphate-buffered saline (PBS) supplemented with 1 mg/ml collagenase type I (Thermo Fisher, Waltham, US). The supernatants were filtered through 100 μm pores of a cell strainer (Greiner Bio-One ...
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bioRxiv - Cell Biology 2019Quote: Equal numbers of cells were incubated for 1 h at 37 °C with either 1 μM of the membrane dye DiI (Invitrogen, Thermo Fisher Scientific) or 1 μM calcein-AM (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were incubated 1 h at 37 °C with secondary donkey anti-rabbit antibody conjugated to Alexa Fluor 488 (1:200, Invitrogen, Carlsbad CA, USA) in blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were preincubated in 3% Bovine serum albumin-PBS at room temperature for 1 h and incubated overnight at 4°C with primary antibodies against CC-ORF (rabbit anti-GFP, polyclonal, Invitrogen A11122, 1:1000). The sections were then incubated with the corresponding fluorescein-conjugated secondary antibodies (Alexa Fluor 488 anti-rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Germany) were grown at 37°C and 5% CO2 in a 1:1 mixture of Opti-MEM + Glutamax (Thermo Fisher Scientific; 51985-026) and DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 h incubation at 25 °C in a light-tight box with Streptavidin-Cy3 (1 μg/mL, Thermo Fisher Scientific, 10768023) and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and 40 cycles of 95°C for 15 sec and 60°C for 1 min using 100 ng of genomic DNA in 1× TaqMan Universal PCR master mix (Life Technologies, Carlsbad, CA) in a 10 μl volume ...
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bioRxiv - Cell Biology 2023Quote: ... Cortical tissue was cut into 1 mm3 fragments and digested with 1 mg/ml collagenase type II at 37° C in DMEM (Invitrogen, Grand Island, NY) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained for 1 h at 37°C with Alexa Fluor 488-labeled chicken anti-rat IgG (Invitrogen, A21470, 1:300) and Alexa Fluor 546-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then incubated overnight at 4°C with the following primary antibodies diluted in 1% Normal Goat Serum: Rat anti-Ki67 conjugated FITC (1:50, Invitrogen, 11-5698-82), Mouse anti-active/pro-caspase3 (1:20 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated overnight at 4°C with the following primary antibodies: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), anti-TIAR (BD Transduction ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and incubated overnight at 4°C with gentle rotation with 40 μl Pierce Anti-c-Myc Magnetic Beads (ThermoFisher Scientific Cat# 88842) or anti-Flag® M2 Magnetic Beads (Merck Cat# M8823 ...
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bioRxiv - Neuroscience 2022Quote: ... sh-c-Jun and c-Jun over-expressed (pCMV-c-Jun) plasmid were transfected into cells using lipofectamine 3000 reagent (Invitrogen; Carlsbad, CA, USA) according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were washed three times again in PBS with 0.2% Triton X-100 and transferred into secondary antibody solution (anti-chicken Alexa Fluor 488, 1:200; anti-mouse Alexa Fluor 647, 1:200; anti-rabbit Alexa Fluor 647, 1:200; Molecular Probes, Eugene, OR, USA) and incubated for 48 h at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... The Feed C regime used commercial CD EfficientFeed™ C AGT™ (Invitrogen, UK), whereas the U and U40 feeds aimed to provide growth-limiting nutrients (glucose and amino acids ...
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bioRxiv - Microbiology 2021Quote: ... Purified mouse IgGs at w11 were obtained using a 0.2-ml protein G spin kit (Thermo Scientific) following the manufacturer’s instructions and assessed in pseudovirus neutralization assays ...
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bioRxiv - Biochemistry 2020Quote: ... RNA from mouse WAT or liver was reverse transcribed with First Strand cDNA synthesis kit (Thermo Scientific) and amplified with Phusion High fidelity DNA Polymerase (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from mouse midbrain punches using RNAqueous®-Micro Kit RNA isolation (Thermo Fisher Scientific). Ventral midbrain samples were bilaterally collected from 300µm acute coronal mouse midbrain slices using a 1.0mm2 tissue punch ...
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bioRxiv - Neuroscience 2020Quote: ... A cDNA library was obtained from E15.5 mouse brain using a cDNA synthesis kit (K1681, Thermo Scientific). Then the corresponding cDNA of Pou3f1 was produced with this cDNA library ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from mouse ES cells using the GeneJET Genomic DNA Purification Kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from sorted mouse germ cells was extracted using mirVana miRNA isolation kit (Thermo Fisher, AM1560) and used to prepare a library of 5′ monophosphorylated long RNAs as described {Wang et al. ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted from mouse lung or cultured cells using the PureLink RNA Mini Kit (Invitrogen) and cDNA was synthesized from 1μg of RNA using an iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: In vitro: DCs were enriched from spleen and mLN using the Dynabeads Mouse DC Enrichment kit (Invitrogen) and naïve CD8 T cells from OT-I donors were isolated using the Dynabeads Untouched Mouse T Cells kit (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Luminex assay was performed on serum using Mouse 48-plex Procarta kits (EPX480-20834-901; Thermo Fisher) according to manufacturer’s protocol and modifications as previously described51 ...
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bioRxiv - Cell Biology 2023Quote: The 5’ end of the mouse Pdgfrβ i10 splice variant was elucidated using the GeneRacerTM Kit (Invitrogen). The P90 WT (c57BL/6 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted from E15.5 wildtype mouse kidneys using the Purelink RNA mini-kit (Invitrogen, Cat#12183025) and reversed transcribed using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were 1:400 anti-rabbit Alexa 488 and 1:250 anti-mouse Alexa 594 from Invitrogen. The cells were washed thrice in 1X PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-RBPMS (1:200; 91. Secondary antibodies used were: Alexa Fluor 488 donkey anti-mouse (1:500; ThermoFisher, A32766); Alexa Fluor 488 donkey anti-rabbit (1:500 ...
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bioRxiv - Genetics 2021Quote: ... Alexa Fluor 488 Goat Anti-mouse IgG (1:300) and Alexa Fluor 568 Goat Anti-rabbit IgG (1:300) from Invitrogen. Digital images were obtained using Leica TCS SP8 LIGHTNING confocal microscope equipped with motorized stage and LAS X software ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: mouse anti-brp (1:50, Developmental Studies Hybridoma Bank #nc82) and rabbit anti-GFP (1:1000, Invitrogen), with secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... and then immunostained with mouse anti-NaV1.1 (1:200; UC Davis/NIH NeuroMab Facility; K74/71) and Alexa 488-conjugated anti-mouse IgG1 secondary antibodies (ThermoFisher). Images were acquired with a Zeiss 700 confocal ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... (IF 1:200) and Alexa Fluor 647-conjugated polyclonal chicken anti-mouse antibody (A21463) (IF 1:200) were purchased from Invitrogen. Alexa Fluor 488 phalloidin was purchased from Invitrogen (whole-mount stain 1:200) ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the recommended dilution of HRP-conjugated secondary antibody (goat anti-rabbit IgG HRP 1:10000, Invitrogen; goat anti-mouse IgG HRP 1:10000, Invitrogen) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with anti-mouse or anti-goat Alexa Fluor 488 and Alexa Fluor 594 antibodies (1:400; 1 hr at RT; Invitrogen) and counter-stained with DAPI nuclear dye (0.5 µg/ml in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Alexafluor 488 F(ab’)2 fragment of goat anti-rabbit IgG (H+L) (1:100) and Alexafluor 488 goat anti-mouse IgG (H+L) (1:100) were all purchased from ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... using the appropriate antibody (1:10,000; rabbit polyclonal PA1-16889, Thermo Fisher or 1:10,000; mouse monoclonal MA1-140, Thermo Fisher) alongside with the targets of interest ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight with primary antibody (Thermo Fisher rabbit anti-GFP, 1:1,000; Thermo Fisher mouse anti-HA, 1:5,000). HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... or mouse antibody to Myc (1:2000, Sta. Cruz) and Clean-Blot IP detection reagent (HRP) (1:5000, Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... and washed in PBS two additional times before adding secondary anti-goat Cy5 (1:2500, 072-02-13-06, KPL) or anti-mouse Alexa Fluor 647 (1:2500, A32728, Invitrogen) and incubated at 4°C in the dark for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 hour incubation with Alexa Fluor 647-conjugated secondary antibody (donkey anti guinea pig, 1:1000) or Cy3-conjugated secondary antibody (goat anti mouse,1:1000, Invitrogen) respectively.
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bioRxiv - Bioengineering 2021Quote: ... Samples were rinsed once with PBS++ and incubated with 1:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488 or 633 phalloidin for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then washed and incubated for 1 min at RT with 1:5000 anti-mouse secondary antibody conjugated via Streptavidin with Qdot655 (Invitrogen) in Krebs solution supplemented with 10% Horse Serum and 1:1000 DMSO or forskolin ...
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bioRxiv - Cell Biology 2022Quote: ... followed by washing with Tween-20/PBS and 1 h incubation with HRP-conjugated goat anti-mouse IgG at 1:40 000 (Cat# A16066, Invitrogen) in 100 µl per well of Tween-20/PBS ...