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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for Mouse Anti Hepatitis C Virus Core Protein Antibody 1862 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes were labelled with chicken anti-glial fibrillary acidic protein (GFAP) polyclonal antibodies (Thermo Fisher PA1-10004, 1:500-1000). Microglia were labelled using rabbit anti-Iba-1 monoclonal antibody (Abcam ab178846 ...
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bioRxiv - Genomics 2022Quote: ... The purified protein was analyzed by SDS Page electrophoresis and western blot analysis using anti-His antibodies as per manufacturer guidelines (ThermoFisher).
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bioRxiv - Molecular Biology 2022Quote: ... or anti-TBP (from Steve Buratowski lab) antibody and then bound to magnetic beads (Dynabeads protein G, Life Technologies #10004D). The beads were washed twice with PBS and once with PBS-T and the cross-links were reversed by incubating in Tris-EDTA+1% SDS at 65°C for 14 h ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell or nuclear extracts were incubated with 2.5 µg of anti-FLAG antibody bound to 50 µl Dynabeads Protein G (Invitrogen #10003D) overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... and rabbit anti-H1.8 custom antibodies (26) (Identification# RU2130) were conjugated to Protein-A coupled Dynabeads (Thermo Fisher Scientific, # 10001D) at 250 μg/ml beads at 4 °C for overnight on a rotator ...
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bioRxiv - Microbiology 2024Quote: ... in the cell culture supernatants as well as by detecting the corresponding proteins in cell lysates after standard Western immunoblotting using a commercial anti-V5 monoclonal antibody (SV5-Pk1 from Invitrogen).
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bioRxiv - Immunology 2024Quote: ... and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Immunology 2023Quote: ... HIF1α protein was detected using an anti HIF1α antibody directly conjugated to Dylight650 (MA5-16008,ThermoFisher Scientific, Waltham, MA, USA) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Blockage of EV HSP70 protein was performed by the pre-incubation of EVs with 1 μg/ml anti-HSP70 antibody (Invitrogen) [32] in high glucose DMEM (4.5 g/l ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Cell Biology 2022Quote: ... Protein expression was monitored by immunoblotting with a 1:1000 dilution of Anti V5 Antibody (Fisher Scientific Catalog # R960-25) or a 1:1000 dilution of Anti-GFP polyclonal Antibody (Product # PA1-980A) ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with an anti-TasA antibody (rabbit) at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (Thermo Fisher). A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the following primary antibodies: mouse polyclonal antibodies to HuC (1:1000, Invitrogen) and Myc (1:1000 ...
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bioRxiv - Biophysics 2023Quote: Primary antibodies used were: Mouse-beta tubulin monoclonal antibody (Thermo Fisher Scientific, 32–2600), Rabbit-anti-Tom20 polyclonal antibody (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: cardiac troponin T mouse monoclonal antibodies (cTnT: Thermo Scientific, Rockford, IL, USA), diluted to 1/200 with 3% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro-BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
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bioRxiv - Genomics 2020Quote: ... diluted chromatin was incubated overnight at 4°C with Protein A and Protein G Dynabeads (ThermoFisher Scientific 10002D, 10004D; 25µL each) and indicated antibodies (Key Resources Table) ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular debris was pelleted for 15 minutes at 4 °C and protein concentration was quantitated with the Coomassie (Bradford) Protein Assay (ThermoFisher Scientific). A total of 15μg (organoids ...
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bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 60°C for 15 min and protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific). The protein mixture (30∼50 µg ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in 1x Laemmli Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23250; ThermoFisher Scientific). Proteins (10µg/sample ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Cell Biology 2023Quote: ... Debris was removed by centrifugation (4°C, 30 min, 20’000 g) and total protein concentrations measured using the Bradford protein assay (Thermo Fisher Scientific). Typically ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysates were centrifuged at 16,400 ×g for 15 min at 4 °C and the protein concentration in the supernatants was measured using a Pierce BCA protein assay kit (ThermoFisher Scientific). Total protein (10 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 21 130 x g at 4 °C for 30 min and protein content quantified using the Pierce BCA protein assay kit (ThermoFisher Scientfic). Twenty µg of the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 for overnight at 4°C and then incubated with respective HRP-labelled secondary antibodies [(anti-Rabbit IgG; 656120; Thermo Fisher Scientific), (anti-Mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in secondary antibody diluted 1:500 in block (donkey anti-rabbit Alexa 568, Life Technologies A10042). Sections were again washed in TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 2008) at 4°C overnight and then incubated with Alexa Fluor 488-conjugated anti-rabbit antibody (1/800, Life Technologies, Oregon, USA). For in situ hybridization of the mouse Mesp1 and Mesp2 mRNA ...
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bioRxiv - Genomics 2020Quote: ... at a dilution of 1:100 overnight at 4°C followed by donkey Alexa488-conjugated anti-rabbit secondary antibody (Thermo Fisher Scientific) at a dilution of 1:200 for 30min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were then incubated overnight at 4°C with primary antibodies at a concentration of 1:500 for rabbit anti-MAP7 (Thermofisher PA5-31782), 1:500 for mouse monoclonal anti-Tau-1 (Millipore MAB3420) ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... OFC sections were reacted with the primary antibody for rabbit met-enkephalin (1:400, Abeam ab22620) for 24h at 4°C and Alexa-fluor 488 goat anti rabbit secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the rabbit anti-wnt8b primary antibody (EMELCA Bioscience) diluted 1:200 at 4°C for 48 hours in Pierce Immunostain Enhancer (Thermo Fisher Scientific). Sections were washed three times with TBS and incubated with Goat anti-Rabbit-biotinylated ...
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bioRxiv - Microbiology 2022Quote: ... GTX135357) primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, Carlsbad, CA, USA) secondary antibodies for 40 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...