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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cells were mixed by inversion and 75µl of the suspension was dispensed to at least triplicate wells of 96-well blacked sided optical bottom plates culture plates (Thermo Scientific). At 40 hours post seeding ...
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bioRxiv - Cell Biology 2021Quote: ... were manually excised using 21G needles/scalpel blades and grown in low-binding 96 well plates (96 well Nunc Sphera Round Bottom Plates, ThermoFisher Scientific) in Retinal Differentiation Media (DMEM/F12 nutrient mix (3:1 ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng circRNAs per well for 24-well plates (2 μg for 6-well plates) were transfected with lipofectamine MessengerMax (Invitrogen, LMRNA003) on the next day when the cell culture must have >90% viability and be 70% confluent ...
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bioRxiv - Systems Biology 2022Quote: ... 1 µl out of 1.1 µl of sample was picked up out of a 384 well plate (Thermo AB1384) placed on an auto sampler height adjuster for PCR plates (ThermoFisher 6820.4089) and sealed by an aluminum foil cover (ThermoFisher AB0626) ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were cultured as spheres on non-tissue culture-treated 10cm plates or as adherent cultures on laminin on tissue culture-treated 10 cm plates (ThermoFisher Scientific). GSCs were grown in DMEM/F12 media ...
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bioRxiv - Synthetic Biology 2019Quote: Each plate containing the isolated clones was used to inoculate 200-µl aliquots placed on two replica 96-well plates for fluorescence measurement (Costar black plates with clear bottom; Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μl of each supernatant was transferred into a 96 well plate and the absorbance at 562 nm was measured using a Varioscan plate reader (Thermo scientific). In order to evaluate the degree of nanoparticle interference ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of samples were transferred to a microwell plate (Nunc™ 3 84-Well Optical Bottom Plates # 242764, Thermo Scientific) and the time course of fluorescence-intensity (see Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... by using a Fluorostar Omega plate fluorescence plate reader (540 nm excitation, 590 nm emission) after incubation with 10% Presto Blue (Life Technologies) for 1h at 37C ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells were pre-cultured to exponential phase in 180 μl of YES in 96-well culture plates at 32°C in the VarioSkan Flash plate reader (Thermo Scientific). 5 μl of the preculture were used to inoculate 175 μl of fresh YES in 96-well culture plates and incubated for ∼48 hours in the VarioSkan Flash plate reader at 32°C with background shaking (1080 spm ...
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bioRxiv - Systems Biology 2022Quote: ... filtered by positive pressure and the flowthrough was collected in a glass coated microtiter plate (WebSeal Plate+ 96-Well Glass-Coated, 300 - Fisher Scientific); 3 ...
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bioRxiv - Neuroscience 2023Quote: ... for ten minutes in a 96-well plate (protected from light) and the fluorescence was read at 520 nm using a Varioskan Flash plate reader (ThermoFisher, UK).
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM) and diluted in DMSO transferred to wells of PCR Plates (Micro Amp Fast 96-well Reaction Plates, Applied Biosystems) to ensure matching final DMSO concentrations.
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial growth was monitored by measuring OD600 and for growth experiments performed in airtight sealed microtiter plates (sealing tape for 96-well plates, Thermo Scientific), a Power Wave XS microplate reader (BioTek Agilent ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μL aliquots of infected cells were deposited in a white 96-well plate (F96 MicroWell™ Plates, non-treated form Nunc) and the luminescent signal was measured with the help of the plate reader.
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bioRxiv - Biophysics 2023Quote: ... 8 µl of ATP-ATPase reaction mix were transferred from row A on master mix plate to respective row (B to H) on reaction stop plate using a MatrixTM Multichannel Pipette (Thermo Fisher). Additionally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then transfected with 100 ng/well (96-well plates) or 500 ng/well (24-well plates) indicated plasmids using Liopfectamine 3000 (Invitrogen, USA) for 24 h or as specified ...
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bioRxiv - Microbiology 2023Quote: ... pre-cultures were prepared by inoculating single colonies from a LB agar plate in 200 μl of liquid LB in a 96-well plate (Nunc, Thermo Scientific). After 22 h of growth at 37°C with 250 rpm shaking ...
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bioRxiv - Microbiology 2023Quote: ... gondii growth were determined in HFF cell cultures using 96-well black plates (Optical-Bottom Plates with Polymer Base, ThermoFisher Scientific). Sulfadiazine (positive control ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Cancer Biology 2023Quote: ... siRNAs were transfected at 10ng/well in 384 well plates and 40ng/well in 96 well plates using Lipofectamine RNAiMAX (Invitrogen, #13778075). Imaging and drug incubation experiments were conducted in 384 well plates ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Plant Biology 2024Quote: ... 3 μl of the dilution series and cocktail mix were dispensed into duplicate wells on a 384-well ABI optical plate and the plate was loaded on the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). The FAM-Non Fluorescent Quencher detector and Rox passive reference were selected ...
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bioRxiv - Immunology 2024Quote: ... monolayer permeability was assessed by transferring the transwell plates in a new receiver plate containing 200 µl Hank’s balanced salt sodium (HBSS) (Gibco 14025-092). To determine permeability of the monolayers (1 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... and GFP fluorescence (excitation 485 nm, emission 520 nm) were measured in microtiter plates using a Varioskan Lux plate reader (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: The proteolytic activity of activated globupain and its substitution variants was assessed using the casein gelzan™ CM plate assay and the EnzChek™ Protease Assay Kit (Thermo Fisher Scientific, MA, USA), respectively ...
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bioRxiv - Cell Biology 2020Quote: The calibration was verified using a phase retrieval algorithm [20] in combination with through-focus scans of 175 nm green fluorescent beads (PS-speck, ThermoFisher, P7220, dilution 1:500) mounted in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed three times with 0.5% Tween 20 in PBS and then incubated with goat anti-rabbit IgG Alexa-488 (1:200; Life Technologies, cat. no. A-11008). DAPI (40,6-diamidino-2-phenylindole ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Tween-20 in Tris-buffered saline followed by overnight incubation with the following antibodies: rabbit-anti-HDAC1 (Thermo Fisher; PA1-860; 1:2000), HDAC1 phosphorylation on serine residue 421 (rabbit-anti-pHDAC1(S421) ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples were centrifuged for 15 min at 12,000 g at 4 °C and the top aqueous layer isolated and 1 μL of RNA-grade glycogen (Thermo Fisher Scientific, 20 mg/mL) and 375 μL of isopropanol were added ...
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bioRxiv - Neuroscience 2021Quote: ... 14 mm glass-bottom dishes were first incubated with 40 μg/ml poly-D-lysine (PDL) in PBS at room temperature for 1 h and subsequentially with 20 μg/ml laminin (Fisher Scientific; 23-017-015) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in SNET Digestion buffer (20 mM Tris, 5 mM EDTA, 400 mM NaCl, 1% w/v SDS) with Proteinase K (Thermo Fisher Scientific, Pittsburgh, PA) overnight at 55 °C with intermittent shaking ...
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bioRxiv - Bioengineering 2021Quote: ... three different volumes (20 µL, 40 µL, and 60 µL) of a suspension of 1-µm diameter fluorescent particles (FluoroMax, Thermo Fisher Scientific Inc., USA) at a concentration of 2×107 particles/mL was added in the inlet reservoirs ...
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bioRxiv - Physiology 2019Quote: ... Myoblasts were let to proliferate in growth medium (F12/DMEM, 25 mM glucose, 20% FBS, 1% penicillin-streptomycin [Anti-anti, Thermo Fisher Scientific, Stockholm, Sweden]). Differentiation was induced in DMEM/M199 media containing HEPES (0.02 M ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were washed in PBS after fixation and stained for 2 hours at room temperature with Alexa Fluor 488 Phalloidin (Thermo Fisher #A12379, 1:20). Afterwards the samples were washed once with PBS and mounted in Fluoromount-G ...
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bioRxiv - Biochemistry 2019Quote: ... Blots were incubated over-night with primary antibody diluted in PBST (phosphate buffered saline with 1 mL/L Tween-20/PBS) + 5 % BSA (Thermo Fisher Scientific, Roskilde, Denmark) at 4°C ...
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bioRxiv - Genomics 2019Quote: ... the tissues were washed thrice in PBT for 20 minutes each and incubated in Alexa fluor 568 goat anti-mouse (1:200, Invitrogen Molecular Probes, Carlsbad, CA, USA) for two hours with constant agitation ...
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bioRxiv - Neuroscience 2021Quote: ... for at least 20 min at room temperature before staining with a fluorescent myelin stain (FluoroMyelinTM Green; 1:300 in PBS; Molecular probes, Eugene, OR, USA) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.3 to 1.75 x 105 cells/per well were seeded in 12-well plates adding a mixture that was pre-incubated at room temperature for 20 minutes and contained 2 µl of lipofectamine and 1 µl of 20 µM of siRNA in 200 µl of Opti-MEM™ (Gibco, Catalog no. 31985062). The mixture was added to 800 µl of complete medium and cells.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were blocked for 1 hour at room temperature with 5% non-fat milk in 1X tris-based saline-Tween-20 (TBST, Thermo Scientific, Cat. No. 28360). Membranes were incubated overnight at 4°C with primary antibodies probing Spectrin and enzyme-mediated degradation products (mouse anti-Spectrin ...
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bioRxiv - Physiology 2024Quote: ... water bath for 3 minutes at 40% power and then mixed with a 1 to 20 solution of Lipofectamine 2000 (Thermo Fisher Scientific, cat. 11668019) and Opti-MEM ...
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bioRxiv - Immunology 2024Quote: ... 5 ml of the total nucleic acid eluate was added to a 20-ml total-volume reaction mixture (1x TaqPath 1-Step RT-qPCR Master Mix, CG; Thermo Fisher Scientific, Waltham, MA), with 0.9 mM each primer and 0.2 mM each probe) ...
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bioRxiv - Genetics 2023Quote: ... we increased the SDS concentration to 1 % and added not only Proteinase K (20 mg/µl) but also TCEP (Thermo Scientific 77720, 0.5 M) to a final concentration of 0.01 µM ...
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bioRxiv - Pathology 2023Quote: ... strain were extracted by using the French Pressure cell(100Mpa, 1 passage) with 20 U/ml DNaseI and proteinase inhibitor cocktail (Thermo Scientific, catalog no. 87786). Such nondenatured lysates were subject to immunoprecipitation with GFP-Trap (Chromo Tek ...
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bioRxiv - Biochemistry 2023Quote: ... Streptavidin displacement was visualized by gel electrophoresis on 1% agarose or 4-20% TBE polyacrylamide gels stained with SYBR™ Gold Nucleic Acid Gel Stain (ThermoFisher, cat. no. S11494). The reactions were incubated at 65°C for 5 min in 0.5 M potassium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% FA and injected onto an Acclaim PepMap100 C-18 trap column (5 µm, 100 µm x 20 mm, Thermo Scientific, Sunnyvale, CA, USA) for sample desalting ...
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bioRxiv - Genetics 2023Quote: ... we increased the SDS concentration to 1 % and added not only Proteinase K (20 mg/µl) but also TCEP (Thermo Scientific 77720, 0.5 M) to a final concentration of 0.01 µM ...