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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and 5% normal goat serum (Invitrogen) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: Dil solution (5 μM; Invitrogen/ThermoFisher) was added to PBS containing EVs and incubated for 30 min ...
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bioRxiv - Cell Biology 2024Quote: Dil solution (5 μM; Invitrogen/ThermoFisher) was added to PBS containing EVs and incubated for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
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bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Microbiology 2019Quote: ... A final washing step after 1 h incubation at TR was followed by addition of 100 µl 1-Step Slow TMB-ELISA substrate (Thermo Scientific) to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 48 h and cytoplasmic DNA was extracted according to the protocol as previously described.35 The cytoplasmic DNA lesion and plasma levels of DNA damage were measured using the DNA Damage Competitive ELISA (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were collected for ELISA assays and RNA was extracted from infected cells using Trizol (ThermoFisher; catalog no. 15596026) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies; Grand Island ...
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bioRxiv - Molecular Biology 2023Quote: ... content in isolated mitochondria from WT and APP cells was measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer instructions (ThermoFisher khb3544).
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bioRxiv - Molecular Biology 2023Quote: ... except for antibodies used for detection in ELISA and flow cytometry. Genetic constructs were synthesized by Eurofins Genomics. Plasmid DNA midiprep (cat. K210004) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Using the standard curve from the ELISA and the amount of gDNA/sample (quantified by Nanodrop Spectrophotometer from Thermo Fisher Scientific), percentage of methylated DNA (5-mC ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was taken at 590 nm with a reference filter of 620 nm using an ELISA reader (Multiskan Spectrum Microplate Reader, Thermo Scientific). All data were normalized against DMSO blank controls.
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bioRxiv - Biochemistry 2024Quote: IL-6 secretion from THP-1-derived macrophages was quantified in the growth media using enzyme-linked immunosorbent assay (ELISA; Invitrogen, #BMS213INST) following the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2021Quote: ... We then ligated an RNA oligo at the 5’-end of the mRNA using GeneRacer Advanced RACE kits (Thermo Fisher Scientific K.K., Tokyo, Japan). For 30-RACE analysis ...
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bioRxiv - Genomics 2022Quote: Complementary DNA (cDNA) was synthesized from 5 μl of RNA sample using a SuperScript VILO with DNAse cDNA Synthesis Kit (Life Technologies, Carlsbad, California, USA) and a ProFlex™ Base (Life Technologies holding Pte Ltd ...
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bioRxiv - Genomics 2020Quote: ... Genomic PCR products obtained with primers 5’-CGCCATCAACTTCACCTAGC-3’ (forward) and 5’-TCTGGGAAGAAGTTTGGCCT-3’ (reverse) were sequenced using the BigDye® Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Massachusetts, USA) on an ABI 3130xl Genetic Analyzer (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products containing the T7 promoter at the 5’ ends of each strand were used for dsRNA synthesis following the MEGAscript RNAi kit (Ambion, Foster City, CA USA) protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We then ligated an RNA oligo at the 5’-end of the mRNA using GeneRacer Advanced RACE kits (Thermo Fisher Scientific K.K., Tokyo, Japan). For 3ʹ-RACE analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Bioengineering 2021Quote: ... sgRNA LA93527 was generated from a PCR DNA template (overlapping primers LA935 5’-GAAATTAATACGACTCACTATAGGACAGTGCGGTCCG-CAAGGGTTTTAGAGCTAGAAA-3’ and LA137 5’-AAAAGCACCGACTCGGTGCCA-CTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’) containing the T7 promoter using the MEGAscript T7 Transcription kit (ThermoFisher Scientific, Walthum, MA USA). The reaction mix was incubated at 37°C for 16 hours and purified using the MEGAclear Transcription Clean-Up kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: We estimated hemocyte differentiation by visualizing and quantifying the synthesis of new DNA in vivo based on 5-ethynyl-2′-deoxyuridine (EdU) incorporation and subsequent detection using the Click-iT EdU Alexa Fluor 647 kit (Invitrogen, Grand Island, NY, USA) as previously described (36,37) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were subsequently re-solubilized in 40 uL of 5% SDS (v/v) and assayed for protein using the PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). 130-170ug of protein/sample (in a total volume of 33 uL) ...
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bioRxiv - Cell Biology 2023Quote: ... macrophages were labeled with 5-EU for 1 hour and detected using the Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit (Invitrogen™ C10330). The images were captured using the Leica SP8 TCS STED 3X microscope with lightning.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again two times in 1XPBS 5’ each and incubated with Alexa Fluor Tyramide 594 (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + hydrogen peroxidase 3% (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... We then used the linearized MLM3613 as template to synthesize the 5′ capped and 3′ polyA-tailed Cas9 mRNA using the mMESSAGE mMACHINE® T7 Ultra Kit (ThermoFisher, cat no: AM1345) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Each pooled seagrass sample was homogenized in a BashingBead Lysis Tube containing 2 mm ceramic beads and 800 μL RNA lysis bu?er (provided in the kit) for 5 minutes at maximum speed using a FisherbrandTM Bead Mill 4 Homogenizer (Fisher Scientific, Waltham, MA, USA). Tissue homogenates were centrifuged at maximum speed (21,130 g ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μl of such mixtures were loaded on 96-well white plates (Microamp Optical 96-well Reaction Plate with Barcode, Applied Biosystems, #4306737), containing 5 μl of an appropriate 5x buffer ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Molecular Biology 2019Quote: ... The experiments were carried out using the CLARIOstar plate reader (BMG Labtech; settings: orbital reading 4 mm (for 96-well plates from Nalge Nunc International) or 2 mm (for 96-well plates from Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... The solution was transferred into a fresh plate and absorbance measured at 590 nm with a plate reader (Multiskan Go, Thermo Scientific, USA). Results were exported with the software SkanItTM (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... Microtiter plate cultivations were performed in sterile 96-well plates (flat bottom Nunclon™ Delta Surface, Thermo Fisher Scientific, Waltham, MA, US) containing 200 μL LB per well ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed three times in DPBS and 100 μl of DPBS was added to each well and plates were sealed with adhesive plate tape (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2021Quote: ... the content of the wells was homogenised by pipetting up and down and 200 μL of suspension were transferred into a microplate-reader compatible plate (Fisherbrand 96-Well plates, Fisher Scientific, USA). The OD was measured at 600 nm (Infinite M200 PRO ...
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bioRxiv - Immunology 2021Quote: ... in a 1:1 bead to T cell ratio and cultured in 2 ml (24-well plate) or 1 ml (48-well plate) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... The unlabeled tumor cells and transduced T cells were added to a 96 Well Black Plate glass bottom plate (Thermo Scientific, 160376). 60-70 z-stacks (0.2μm steps ...
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bioRxiv - Systems Biology 2022Quote: ... cells were seeded at 8000 cells/well (96 well plate) or 35000 cells/well (24 well plate) in media without phenol red (Thermo Fisher 11054020) with glutamine supplemented (Life Technologies 35050-061) ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Biochemistry 2024Quote: ... The Multiplate 96-Well PCR Plate is then incubated at 37 °C in a 96-well Non-Skirted PCR Plate Block (Thermo Scientific #88870120) in a digital dry bath (Thermo Scientific #88870006).
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: dLN single-cell suspensions from C57BL/6 infected mice were cultured in 96 well plates “U” bottom with 5μg/mL of plate-bound anti-CD3 (clone 145-2C11, Invitrogen, Waltham, MA, USA), 0.5 μg/mL soluble anti-CD28 (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The assays were conducted in sterile Nunclon® 96 microwell plates and Nunc® 384 well polystyrene plates purchased from Thermo Fisher Scientific ...