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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 4 µL of CellRox DeepRed reagent (ThermoFisher Scientific) was added directly to the cell culture media in each well ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1mg/mL of Collagenase 4 (Gibco, 17104019) for 20 minutes at 38°C ...
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bioRxiv - Microbiology 2024Quote: ... and quantified using a Qubit 4 Flurometer (Invitrogen). A quality control step on 1% agarose gel with guanidine thiocyanate and ethidium bromide was done ...
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bioRxiv - Bioengineering 2024Quote: ... devices were fixed with 4% paraformaldehyde (PFA, ThermoFisher) with 0.1% Triton-X 100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Samples were fixed using Pierce 4% PFA (ThermoFisher), permeabilized with 0.25% Triton X1000 (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... 4 mM L-glutamine (Life technologies 25030-081), 100 U/ml penicillin/streptomycin (Life technologies 15070-063 ...
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bioRxiv - Plant Biology 2024Quote: ... and Qubit 4 fluorometer (Thermo Fisher, Mumbai, India). The NebNext Ultra II RNA library prep kit (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the samples were fixed with 4% paraformaldehyde (Invitrogen) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed in 4% PFA (Fisher Scientific), washed in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 2X Fluo-4 calcium assay reagent (Thermo Fisher), and preweighed ...
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bioRxiv - Microbiology 2024Quote: ... A Qubit 4 Fluorometer (Invitrogen, Carlsbad, CA, USA) and the 4150 TapeStation system (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... for 4 hours using Lipofectamine 2000 (Invitrogen, USA) and then were grown in DMEM/F-12 medium overnight.
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bioRxiv - Cell Biology 2024Quote: ... 4 mM Glutamine (Glutamax; Gibco Thermo Fisher Scientific), 2 mM Pyruvate and 200 μM Uridine (permissive complete medium ...
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bioRxiv - Molecular Biology 2024Quote: ... NuPAGE 4-12% Bis-Tris gels (Thermo Fisher) were loaded with protein samples and SDS-PAGE was conducted at a constant 180V for approximately 1hr ...
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bioRxiv - Physiology 2024Quote: ... or 4% paraformaldehyde (ThermoFisher Scientific, Waltham, MA, USA) for 2 hours at 4°C for labeling with anti-HuC/D and anti-IL-17R or anti-nestin ...
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bioRxiv - Developmental Biology 2020Quote: ... antibodies were diluted 1:500 in PBT with 1% DMSO and 1% BSA and applied together with TO-PRO-3 (T3605, Thermo Fisher, Waltham, MA) diluted 1:1000 over- night at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% (v/v) Triton X-100) and analyzed by SDS-PAGE on 4-12% NuPAGE Bis-Tris gradient gels (Invitrogen) followed by immunoblotting ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in Alexa488 Streptavidin for 2-4 hours in the same incubation solution at room temperature (S32354, Invitrogen). After final PBS washes ...
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bioRxiv - Biochemistry 2022Quote: ... at a concentration of 2 x 105 cells per ml (4 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were desalted into reaction buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl and 4 mM Tris (2-Carboxyethyl) Phosphine (TCEP) (Invitrogen, T2556)) for removing DTT in storage buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher) at 4°C and 100% humidity ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were used at 1:200 dilution in PBT for 2 hours at room temperature or at 4 °C overnight (Goat anti-Chicken Alexa 488, ThermoFisher Scientific cat#A11039 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated with poly(I:C) at a final concentration of 2 μg/mL using 4 μL Lipofectamine 2000 (Thermo Fisher). At three hours post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... The 4-micron Dionex IonPac AG-11-HC guard column (2 × 50 mm) and 4-micron IonPac AS11-HC analytical IC column (2.0 × 250 mm) were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Immunology 2020Quote: ... CAR T cells were incubated for 30 min at 37°C with either 1μM fura-2 AM or fluo-4 AM (Life Technologies). T cells were then washed in HBSS and resuspended in TexMACS complete medium with 3% AB serum ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated for 30 minutes at 4°C with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher Scientific) while in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were plated in 10cm dishes at 80-90% confluency the day before transfection and transfected 16 hours later with 4 μg total DNA (2 μg of each plasmid) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The cells were placed onto 4 ml MSgg agar (1.5% w/v) set into each chamber of LabTek II 2-chamber microscope vessels (Thermo Scientific) set into various containers ...
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bioRxiv - Cancer Biology 2019Quote: ... The FT tissue was cut into small pieces of around 2-4 mm in length and incubated in pre-warmed Digestive Medium containing Advanced DMEM/F12 (Gibco), 12mM HEPES (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in 2% BSA/PBS were incubated overnight at 4°C and secondary antibodies (Thermo Fisher Scientific, Alexa Fluor) were incubated for 2 hours at room temperature and washed with PBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: Adult female flies were fed for 2-4 days on wet yeast paste and ovaries hand dissected in Grace’s Insect Medium (Life Technologies). Ovaries were fixed in 4% formaldehyde (37% formaldehyde diluted in PBST (0.2% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Whole brains were removed and immediately placed in 4% low-melt agarose mixed 50:50 with Neurobasal media plus B27 supplement (2% agarose final solution) (Thermofisher). Once solidified ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then incubated overnight at 4°C and under agitation with 2 mL Ni-NTA resin (50% v/v, Thermofisher) pre-equilibrated with lysis buffer.
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bioRxiv - Molecular Biology 2021Quote: ... 10’000 rcf for 30 min and then ultracentrifuged at 120’000 rcf at 4 °C for 2 h (TH 64.1 rotor, Thermo Fisher Scientific). The epididymosomal pellet was then washed in PBS at 4 °C and ultracentrifuged at 120’000 rcf at 4 °C for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 2×104 cells were resuspended in 1.5 ml of growth medium containing 0.4% agarose (4% Agarose Gel, Gibco; top layer) and plated on 1 ml bottom layer containing growth medium and 1.2% agarose in a 12-well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... PABP-GFP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-GFP (clones 19F7 and 19C8 ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Immunology 2022Quote: ... with surrounding necrotic areas being carefully removed and were cut into small pieces of 2–4 mm and reserved in the mixture of 1X DMEM medium (Gibco) and Penicillin-Streptomycin solution (Hyclone) ...