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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Cancer Biology 2021Quote: Cells were washed two times in cold PBS and harvested on ice with M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, #78501), plus freshly added Turbonuclease (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: Cell lysate proteins from each iPSC time point were extracted by commercial RIPA or M-Per tissue lysis buffer (Thermo Fisher Scientific) followed by brief pulses of sonication with typically 6 pulses at 20% amplitude followed by 5 sec cooldown on ice ...
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bioRxiv - Biochemistry 2019Quote: ... The protein concentration was determined after denaturing the proteins with 6 M guanidine hydrochloride by measuring absorbance at 280 nm and 496 nm with a NanoDrop Spectrophotometer (Thermo Fisher Scientific). The concentration was calculated using the following equation:
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... was captured using a GFP binding protein (GBP) covalently conjugated to carboxylic acid decorated Dynabeads (Dynabeads M-270 carboxylic acid, Thermo Fisher Scientific). Untagged SMO (Fig ...
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bioRxiv - Biochemistry 2021Quote: ... IP were performed by incubation of lysates 4 h at 4 °C on a rotating wheel with either 50 µL of DynaBeads M-280 protein A magnetic beads (Thermo Fisher Scientific), pre-loaded with 8 µg of rabbit control (Dako ...
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bioRxiv - Immunology 2020Quote: ... Cells from spleen and BMM were washed 3 times with ice-cold PBS and lysed by M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific) in the presence of protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... The ovarian tissues were homogenized with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA) supplemented with Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific). Following centrifugation (16,000 g ...
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bioRxiv - Neuroscience 2023Quote: ... The protein pellets were resuspended in 6 M guanidine solution prepared in 100 mM triethylammonium bicarbonate (TEAB) buffer (Thermo Scientific, Cat# 90114). The protein solutions were reduced with 5 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2023Quote: ... Collected proteins were incubated overnight with 100 μl of streptavidin-coupled Dynabeads (Dynabeads M-280 Streptavidin, Thermo Fisher Scientific, Waltham, MA, USA) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein lysates for immunoprecipitation were generated by resuspending cells in ice-cold GFP-Trap buffer [M-PER mammalian protein extraction reagent (Thermo Fisher Scientific), 1x complete Mini protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Immunology 2022Quote: ... B220 (CD45R, APC-e Fluor 780, eBioscience; clone RA3-6B2) CD11b (super bright 600, Invitrogen, APC-e Fluor 780; clone M1/70) CD11c (APC-e Fluor 780 ...
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bioRxiv - Microbiology 2022Quote: ... M(GFP)NE or M(GFP)NES were diluted (1:20) in transparent DMEM Solution (Gibco) and put into contact with the live cells ...
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bioRxiv - Cell Biology 2023Quote: ... Magnesium 1 M (cat: AM9530G) and Sodium chloride 5 M (cat: AM9759) were obtained from Ambion. Ultrapure water (cat ...
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bioRxiv - Microbiology 2023Quote: ... 0.018 M of trisodium citrate salt (Fisher Chemical) and 3.8 M of ammonium sulfate (ACROS ORGANICS) dissolved in ultrapure water ...
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bioRxiv - Developmental Biology 2021Quote: ... E-cadherin (1: 500; Thermo Fisher Scientific, 13-1900), NKX2.1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... e) Qubit quantification (Thermo Fisher Scientific, Waltham, MA, USA), f ...
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bioRxiv - Developmental Biology 2020Quote: ... Rat monoclonal E-cadherin antibody (Thermo Fisher, 13-1900) was used at 1 µg/mL for all other E-cadherin immunostaining and at 0.5 µg/mL for Western blotting ...
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bioRxiv - Developmental Biology 2021Quote: ... E-cadherin (1: 1500, Thermo Fisher Scientific, 13-1900), NKX2-1 (1 ...
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bioRxiv - Immunology 2021Quote: ... Positive wells were determined using E-Gels (Invitrogen, #720841) and sequenced using Illumina MiSeq Nano 300 V2 cycle Kits (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... and placing it in Hibernate E (Thermo Fisher, A1247601) on ice until cortices were harvested ...
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bioRxiv - Neuroscience 2021Quote: ... EGF-Alexa647 (1:300; Molecular Probes, Cat# E-35351), and biotinylated anti-mCD133 (rat monoclonal ...
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bioRxiv - Synthetic Biology 2021Quote: ... 4 % agarose EX E-gels were purchased from ThermoFisher Scientific (Cat # ...
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bioRxiv - Bioengineering 2020Quote: Cells were washed with carbogenated William’s E (Gibco; A1217601) (adjusted pH 7.4 at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (1: 1500, Thermo Fisher Scientific, 13-1900) and CD44 (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (1: 1500, Thermo Fisher Scientific, 13-1900), GFP (1 ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were kept in E-8 Flex medium (Gibco) with pen/strep (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-E-Cadherin (1:400, Invitrogen, 13-1900); cell proliferation antibody ...
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bioRxiv - Immunology 2022Quote: ... Platinum-E cells were maintained in DMEM (Gibco #11995) supplemented with 10% fetal bovine serum (Benchmark) ...
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bioRxiv - Cell Biology 2022Quote: ... anti-E-cadherin (20 μg/ml, Invitrogen #13-1900), anti-Par6 (1 μg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... we visualize libraries on 2% agarose E-Gel (ThermoFisher) and determine the relative amplification success at the expected ~627 bp band size (amplicon + spacer + all primer sequences + linker) ...
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bioRxiv - Neuroscience 2020Quote: ... in 15 mL Hibernate-E media (Thermo Fisher Scientific) at 37°C for 15 min ...
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bioRxiv - Immunology 2021Quote: Plat-E cells were dissociated by TrypLE reagent (Gibco) before FACS staining while C7 cells were stained directly ...
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bioRxiv - Neuroscience 2023Quote: ... E-18 embryonic rat cortical astrocytes (Gibco, 4×106) were seeded in a 6-well plate at a cell density of 200,000/cm2 in Dulbecco’s Modified Eagle Media (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were analyzed by 1.2% E-gel (ThermoFisher) along with E-Gel 1 Kb Plus Express DNA Ladder ...
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bioRxiv - Genomics 2023Quote: ... and the E-Gel Power Snap Electrophoresis System (Invitrogen). Run time was set to 10 minutes and DNA between 150 and 500 bp was excised from the gel and purified using the QIAquick Gel Extraction Kit (Cat ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies against E-cadherin (clone ECCD2, Invitrogen, 13-1900) and c-Jun (Cell Signal Technology (CST #9165 ...
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bioRxiv - Cell Biology 2022Quote: ... and run in an E-Gel PowerSnap system (Invitrogen). Images were acquired using a LI-COR Fc Imager (Odyssey).
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bioRxiv - Biochemistry 2023Quote: ... using commercial gels (Invitrogen, E-Gel EX, 4% agarose). Product containing fractions of adequate purity were pooled ...
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bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... using the E-gel Power Snap Electrophoresis System (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and 8 Units E-PAP (PolyA Tailing Kit, ThermoFisher). Poly-A tailing reactions were incubated at 37°C for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Platinum-E cells (CellBioLabs) and 293FT cells (Thermo Scientific) were cultured in high-glucose DMEM (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... and the E-Gel Power Snap Electrophoresis System (Invitrogen), selecting for fragments 150 - 500 bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were fractionated on 4% agarose E-gels (Invitrogen), and amplicons ranging from 0.2–8 kb were extracted and purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... The E-gel 96 System (Invitrogen, Carlsbad, Ca, USA) was used to visualize the PCR products on 2% agarose gels ...