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Citations for Thermo Fisher :
501 - 550 of 9647 citations for Water soluble CdTe CdSe ZnS quantum dots fluorescence λem 740 nm FWHM 60 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Absorbance was measured at 450 nm and 570 nm using the Multiskan Ascent 354 microplate reader (Thermofisher, Waltham, USA). Concentrations were determined using the standard curve generated using known concentrations of TNFα and IL-6.
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bioRxiv - Biophysics 2020Quote: ... The grids were subsequently coated with 100 nm and 500 nm fluorescent microspheres (TetraSpeck Microspheres, Invitrogen T7279, T7281, USA) at a 500x or 1000x dilution for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The absorbance at 450 nm (signal) and 570 nm (background) was then measured with a microplate photodetector (Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... The OD at 645 nm and 663 nm for chlorophyll a and chlorophyll b were measured with NanoDrop (Thermofisher). The Chlorophyll concentration was calculated with the Arnon’s equations ...
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and transfected with either 20 nM non-target control siRNA or 20 nM AMPKα1 (both Dharmacon) or FoxO3 (Invitrogen) siRNA using Neon Transfection System (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the absorbances of the extracted RNAs at 260 nm and 280 nm were determined spectrophotometrically (Nanodrop, Thermofisher Scientific). RNA samples with purity ratios (A260/A280 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by an enzyme solution consisting of 2 nM Cel7A labeled with 0.5 nM Qdot (Thermo Scientific; Cas: Q10143MP), 5 mM dithiothreitol ...
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bioRxiv - Biophysics 2023Quote: ... expressing either GPI-GFP or YFP-CB1 were then incubated with increasing amounts of QD-NB-conjugates (between 0.1 nM and 1 nM) in live cell imaging solution (#A14291DJ, Thermofisher) until desired QD density was reached depending on cell density and transfection efficiency ...
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bioRxiv - Molecular Biology 2024Quote: ... miRNA mimic (20 nM) and miRNA negative control (20 nM) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA) according to the instructions of the reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... and mitochondria with either 100 nM MTY for 15 min or 100 nM MitoTracker Green FM (MTG, # M7514, Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The z-stack images of orange (540/560 nm) and deep-red (633/660 nm) fluorescent beads (P7220, Invitrogen) mounted in OI or WI media were acquired and processed in SMAP [26] for 3D point spread function (PSF ...
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bioRxiv - Cancer Biology 2023Quote: ... or increasing concentrations of gemcitabine (dose range = 10 nM to 1μM) (Catalog: G6423-50MG, MilliporeSigma) or paclitaxel (dose range = 10 nM to 1μM) (Catalog: AAJ62734MC, Thermo Fisher) was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... was added at a final concentration of 20 nM along with 0.5 μL 100 nm-sized Tetraspeck beads (Invitrogen) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Human onTARGETplus siRNA were diluted to 20 µM stock in RNase-free water and cells were transfected with 10 nM siRNA for 3 days with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher, 13778075) in 6 well format ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample was placed in a one cm plastic spectrophotometer cuvette and absorption spectra recorded from 480-800 nm versus a distilled water blank using a Genesis 10 UV Instrument (Thermo Fisher Scientific). The spectra were analyzed utilizing the Vision Lite software (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Stained samples were enumerated by discrimination based on green fluorescence (530 nm) and side scatter using an Attune® acoustic focusing flow cytometer (Applied Biosystems) (Frank et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and the fluorescence was measured at 450/485 nm excitation/emission with a microplate reader (Varioskan LUX plate reader, Thermo Fisher Scientific). Following data acquisition ...
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bioRxiv - Cancer Biology 2021Quote: ... dasatinib (150 nM) and dexamethasone (500 nM) alone or in combination for 24 hours and total RNA was isolated using Trizol (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... reverse transfection using 80 nM of silencer RNA (siRNA) for GTSE1 and 100 nM of siRNA for EB1 was performed using Oligofectamine (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Z-calibration and sample drift corrections were done using a few 40 nm TransFluoSphere beads (488/685 nm, Life Technologies) as fiducial markers spread on the cell samples.
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor goat-anti rabbit 488 nm, goat anti-rat 594 nm, Invitrogen) for 1 h at RT ...
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bioRxiv - Systems Biology 2021Quote: ... miR-291a-3p (20 nM final concentration), or a combination of both (10 nM final concentration, each) using the Lipofectamine RNAiMax transfection reagent (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... and RNA purity was analysed by comparing 260/230 nm and 260/280 nm absorbance ratios (Nanodrop 2000, Thermo Fisher). RNA was quantified using a Qubit 2.0 fluorometer (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... We choose the excitation and emission wavelengths to be 495 nm and 537 nm (excitation and emission maxima for SYBR Gold, as provided by Invitrogen). For control measurements ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected twice with 10 nM Silencer Select BRCA2 s2084 siRNA and 10 nM Silencer Select BRCA2 s2085 siRNA (Ambion) using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The measurements were performed by a fluorescent reader at 360 nm excitation/460 nm emission (Varioscan, Thermo Fisher Scientific, US). While DMSO was used as solvent control ...
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bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with 20 nM siRNA oligonucleotides and 20 nM negative control siRNA using Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Biophysics 2022Quote: Molten M9DCAA 0.6% agar was mixed with 0.5 µm-diameter fluorescent microsphere (FluoSpheres carboxylate-modified, ex/em: 580 nm /605 nm; Thermofisher F8812) at a final microsphere number density of 3.1 x1011 particles/mL (∼1000-fold dilution of the microsphere solution) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were grown in antibiotic free media and transfected with 20 nM mimic when transfected singly or 10 nM respective mimic for dual transfections using RNAimax reagent (Invitrogen). Cells were harvested 39 hours post transfection and duplicate samples were pooled for protein extraction and subsequent western blot analysis ...
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bioRxiv - Biophysics 2021Quote: Yellow-green carboxylate fluoSpheres (REF F8803, 2% solids, 100 nm diameter, exc./em. 505/515 nm, Invitrogen, Thermo Fisher Scientific) were diluted 1000× in ultrapure water and sonicated for 10 minutes in a water bath sonicator (Labsonic LBS1-0.6 ...
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bioRxiv - Biophysics 2021Quote: Yellow-green carboxylate fluoSpheres (REF F8803, 2% solids, 100 nm diameter, exc./em. 505/515 nm, Invitrogen, Thermo Fisher Scientific) were diluted 1000× in ultrapure water and sonicated for 10 minutes in a water bath sonicator (Labsonic LBS1-0.6 ...
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bioRxiv - Bioengineering 2020Quote: ... The Cas12a-crRNA ribonucleoprotein complex was assembled by mixing 100 nM Cas12a and 100 nM crRNA in 1X NEBuffer 2.1 in the Proflex PCR system (ThermoFisher Scientific) at 25°C for 15 minutes (volume 28.5 µL) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with 20 nM siRNA oligonucleotides and 20 nM Negative Control siRNA using Lipofectamine(tm) RNAiMax (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... 530 and 657 nm (anthocyanins) or 320 nm (phenolic compounds) in a Multiskan Spectrum (Thermo Fisher Corp., Waltham, MA, U.S.A.). Chlorophyll concentrations were calculated using the following equations ...
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bioRxiv - Biochemistry 2020Quote: ... Beta-lactamase activity was determined at 390 nm (yellow) and 486 nm (red) using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... of the system was measured under the same imaging conditions (with a 50 nm z-step size) using 100 nm fluorescent TetraSpeck microspheres (Invitrogen) embedded in 1% agarose gels ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Optical resolution was assessed by imaging fluorescent beads (yellow-green fluorescent beads, 40 nm or 170 nm in diameter, Invitrogen) immobilized on glass slides (Fig ...
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bioRxiv - Biophysics 2023Quote: ... diluted ×5000 in ultrapure water or a solution of Red carboxylate fluoSpheres (REF F8786, 2% solids, 20 nm diameter, exc./em. 580/605 nm, Invitrogen, ThermoFisher) diluted 3000 ×in ultrapure water.
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bioRxiv - Microbiology 2023Quote: ... falciparum parasites were incubated with 1 µM quarfloxin and either 50 nM DRAQ5 Abcam) or 100 nM Lysotracker DeepRed (ThermoFisher) or MitoTracker Deep Red FM (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... RNA was quantified fluorometrically at 260/290 nm and 260/230 nm using NanoDrop and the Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... measurement (ratio of OD at 260 nm to OD at 280 nm is greater than 1.7) using a Nano-Drop spectrophotometer (Thermo Scientific). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 were seeded in twenty-four-well plates for 24 h in the presence of 200 nM of PMA then transfected with 50 nM siRNA using Lipofectamine RNAIMAX (Invitrogen) according to the manufacturer’s instructions and incubated for a further 3 days ...
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bioRxiv - Biophysics 2024Quote: ... 40 nm dark-red beads (catalog no. F8789) and 40 nm red-orange beads (catalog no. F8794) were obtained from Invitrogen. Norland Optical Adhesive 74 (catalog no ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was performed on Machery & Nagel Alugram® xtra SIL G/UV 254 visualization under UV light (254 nm) and/or (366 nm) and/or by dipping in anisaldehyde stain and subsequent heating.Commercial reagents and solvents (Acros Organics, Fluorochem ...
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bioRxiv - Biophysics 2023Quote: ... for different fluorescent color beads (40 nm diameter, red or dark red beads with emission maximum at 605 or 680 nm, F10720, Invitrogen) by illuminating with a 561 nm (or 640 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipophilic tracer 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO, excitation, 488 nm; emission 525/50 nm) was purchased from Invitrogen (Carlsbad, CA, USA). Counting beads for flow cytometry experiments were purchased from Biolegend (424902 ...