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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Rabbit IgG Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Sheared lysates were pre-cleared using 2 µg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 hr at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... ERCC spike mix 2 (Ambion) was added to the RNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated overnight at 4°C and then washed three times with 0.3 % PBST followed by incubation with a horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (Alexa Fluor Plus™ 647 goat anti-rabbit IgG, ThermoFisher, USA; 1:5000) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... anti-IgG antibodies (Invitrogen). Actin was labeled with phalloidin-TRITC (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... anti-IgG antibodies (Invitrogen). Samples were permeabilized with 0.5 % Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... containing a 1/250 dilution of Alexa 488-conjugated anti-Spike antibody (GTX632604 conjugated using the Zenon Alexa Fluor 488 Mouse IgG Labeling Kit, ThermoFisher) and washed 4 times in FACS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse-IgG and anti-rabbit-IgG were from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse IgG 647 and anti-rabbit IgG 568 (Life Technologies) were used at 1:1000 dilution.
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV RBD (residues Arg306-Phe527) were inserted into pFastBac (Invitrogen) with an N-terminal gp67 signal peptide and a C-terminal 6 × His tag ...
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bioRxiv - Microbiology 2019Quote: ... The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon) and goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (Thermo Fisher Scientific). Viral plaques were visualized by incubation with NBT/BCIP solution (Roche ...
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bioRxiv - Zoology 2022Quote: ... while GFP was detected using an anti-GFP rabbit polyclonal primary antibody and an Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific). Cell nuclei were counterstained with DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... the intestines were incubated 2 h at room temperature with secondary antibody Alexa Fluor 488 donkey anti-rabbit IgG (#A21206, Invitrogen) 1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 hours with a secondary goat anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (Invitrogen). The sections were then coverslipped and mounted on microscope slides ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... secondary antibodies were applied for 2 h in the dark (1:250 anti rabbit IgG coupled to AlexaFluor-555 [Invitrogen, A31572] and 1:250 anti mouse IgG coupled to AlexaFluor-488 [Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... antibody and the secondary Alexa Fluor 488 (green) labeled goat anti-rabbit IgG (H + L; F (ab’)2 fragment) (Invitrogen) targeting the anti-E ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation for 2 hours in secondary antibody (Alexa Fluor 633 goat anti-rabbit IgG (H+L) (Invitrogen A21071) 1:250 in block solution) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 antibody (Antibodies Online; catalog no. ABIN6952432) followed by staining with secondary antibody Alexa-647 (goat anti-mouse; Thermo Fisher; A21235) and DAPI for nuclei staining ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV2 spike and RBD were conjugated with Alex647 fluorescence (Thermo Fisher A30009).
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bioRxiv - Cancer Biology 2021Quote: ... primary antibodies were detected using Alexa Fluor 680 F(ab’)2 fragments of goat anti-mouse immunoglobulin G (IgG) or anti-rabbit IgG (Life Technologies) diluted at 1:8,000 in Blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated goat anti-rabbit IgG F(ab’)2 and HRP-conjugated anti-mouse IgG(H+L) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... IAV (H1N1 PR8 strain) and SARS-CoV-2 were grown in Vero cells with VP-SFM medium (11681-020, Gibco). DENV ...
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bioRxiv - Immunology 2021Quote: ... the synthetic DNA encoding the RBD of SARS-CoV-2 was synthesized by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA) and used as template for the amplification of the three RBM epitopes (Table 2) ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from nasopharyngeal swab samples (labeled as S2, S3, S4, S9) collected from SARS-COV-2 infected COVID-19 patients using TRIzol (Invitrogen) reagent following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S and RBD proteins were transiently expressed in Freestyle 293F system (ThermoFisher). In brief ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... extracted DMS-modified RNA from SARS-CoV-2 infected Vero cells was fragmented using the RNA Fragmentation kit (ThermoFisher Scientific). 1.5 μg of rRNA subtracted total RNA was fragmented at 70°C for 2.5 min ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: The codon-optimised full-length SARS-CoV-2 S gene was designed and purchased from GeneArt (Thermo Fisher Scientific, Poland). The gene was cloned into pCAGGS vector sequence verified that was a gift from Xingchuan Huang ...
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bioRxiv - Microbiology 2021Quote: ... As controls we used SARS-CoV-2-S-RBD pre-incubated only with buffer and non-specific mouse serum (Invitrogen). Plates were extensively washed with phosphate-buffered saline/0.05% Tween-20 (PBST) ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... BHK-21 were transfected to express the SARS-CoV-2 S protein using Lipofectamine 3000 (Thermo Fisher Scientific, Madrid, Spain) and after 24h transfected cells were inoculated with a replication-deficient rVSV-luc pseudotype (MOI ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Immunology 2021Quote: ... Each dilution was incubated at 37°C and 5% CO2 for 1 hour with an equal volume of 1000 plaque forming units/ml (PFU/ml) of SARS-CoV-2 (isolate USA-WA1/2020) diluted in DMEM (Gibco) containing 2% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 prefusion stabilized S trimer was incubated with yellow-green streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... were cultured in the presence of SARS-CoV-2-S (9pmol) (PepTivator) in 24-well plates and maintained in IMDM medium (Gibco) supplemented with penicillin (100 units/mL) ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).