Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
-
bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
-
bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
-
bioRxiv - Molecular Biology 2021Quote: ... biotin labelled Hi-C 3.0 DNAs were purified by 20 μl Dynabeads® MyOne™ Streptavidin C1 beads (Thermo Fisher 65002). The biotinylated-DNA on C1 beads was used to perform on-beads library making with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
-
bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
-
bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
-
bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
-
bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
-
bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
-
bioRxiv - Immunology 2022Quote: ... were transfected at cell densities between 8.0×105 to 1.0×106 cells/ml with equal masses of plasmids containing the heavy chain and light chain genes using FreeStyleTM MAX Reagent (ThermoFisher Scientific 16447100); both plasmids were diluted in OptiPROTM SFM (ThermoFisher Scientific 12309050) ...
-
bioRxiv - Microbiology 2021Quote: ... The antibody production was conducted by co-transfection of the light chain and heavy chain sequences into Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific, A14635). The culture supernatant was collected at 7 days post-transfection ...
-
bioRxiv - Immunology 2020Quote: ... Four µg of the heavy chain and 4 µg of the corresponding light chain were mixed together in 375 µl Opti-MEM (Gibco, ThermoFisher Scientific, UK) before addition of polyethylenimine (PEI MAX (Polysciences ...
-
bioRxiv - Cell Biology 2022Quote: Plasmids pcDNA3.4 comprising heavy chains and light chains of antibodies were transiently expressed in ExpiCHO-S cells using the ExpiCHO™ expression system (Thermo Fisher). Briefly ...
-
bioRxiv - Immunology 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were co-transfected by polyethylenimine-transfection to Expi293F™ cell (ThermoFisher, A14527). Cells were cultured at 36.5°C ...
-
bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: Polymerase chain reactions utilized DreamTaq polymerase (ThermoFisher, Waltham, MA) and the supplied reaction buffer components ...
-
bioRxiv - Bioengineering 2023Quote: ... ms anti cardiac myosin heavy chain (Invitrogen, MA1-26180); rb anti – myosin light chain (Cell Signalling ...
-
bioRxiv - Cell Biology 2023Quote: ... or anti clathrin Heavy Chain (MA1-065, Thermo Fisher) at 1 μg mL−1 in PBS blocking buffer with 5% BSA and 0.01 % w/v saponin ...
-
bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
-
bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2019Quote: HEK293-SUMO3m cells were grown in DMEM (Thermo Fisher Scientific) containing light (0Lys ...
-
bioRxiv - Biochemistry 2020Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Invitrogen) with 5% (v/v ...
-
bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were maintained in DMEM (Thermo Fisher Scientific, 10569010) supplemented with 10% FBS and penicillin/streptomycin ...
-
bioRxiv - Neuroscience 2019Quote: HEK293 cells or FreeStyle 293F cells (Invitrogen, Waltham, MA, USA) were cultured in DMEM medium with 10% FBS and 1% Pen-Strep at 37°C with 5% CO2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and pHelper62 into HEK293 cells using Lipofectamine LTX (Invitrogen 15338030) following standard protocols62 ...
-
Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... The construct was transiently transfected into HEK293 cells (Thermo Fisher) with PEI MAX (Polysciences ...
-
bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in DMEM (High Glucose DMEM; Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were cultured in DMEM + GlutaMAX-I (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... HEK293 was grown in DMEM (Thermo Fisher, USA, Cat# 11995065) supplemented with 10% FBS (HyClone ...
-
bioRxiv - Cancer Biology 2020Quote: ... VCaP and HEK293 were grown in DMEM with Glutamax (Gibco); LNCaP ...
-
bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ HEK293 parental cells (Invitrogen) were seeded at 1×106 cells per 10 cm culturing dish in 15 ml media (DMEM High Glucose ...
-
bioRxiv - Immunology 2020Quote: ... HEK293 cells were maintained in DMEM (Thermo Fisher cat. 11965) supplemented with 10% FBS ...
-
bioRxiv - Biochemistry 2021Quote: 0.3×106 HEK293 cells in DMEM media (Gibco #31966-021) supplemented with 10% fetal bovine serum were seeded in each well of 12-well plates ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... Dulbecco’s Modified Eagle’s Medium for HEK293 cells) (Gibco, Thermofisher Scientific) containing 10% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... Dulbecco’s Modified Eagle’s Medium for HEK293 cells) (Gibco, Thermofisher Scientific) containing 10% (v/v ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293-6E cells were transfected using 293fectin protocol (Thermo Fisher). The SIRT6 biosensors were expressed using mammalian expression vector pTT5 (National Research Council ...
-
bioRxiv - Microbiology 2021Quote: ... and RaTG13 RBD were produced using HEK293-F cells (Invitrogen) cultivated in suspension using Freestyle medium (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... HEK293 and COS-7 cells and Lipofectamine 2000 (Thermo Fisher) reagent for NIH3T3 cells ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were maintained in DMEM (Gibco™, #31966-021) supplemented with 10% FBS and 1X Non-essential Amino Acid supplement (Sigma ...
-
bioRxiv - Physiology 2022Quote: ... HEK293 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine Serum (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 transfections were performed with Lipofectamine 2000 reagent (ThermoFisher, #11668019) according to standard procedures and the cells expressing SorLA mutants and TNFα-GFP (or GFP ...