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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 8 2 4 difluorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... We then added 8 μL/well of RT mix to the plate: 4 μL of 5x RT Buffer (Thermo Scientific), 0.35 μL of SUPERase-In RNAse Inhibitor (20 U/μL ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Microbiology 2021Quote: 8 x 103 HeLa-ACE2 cells and 3.2 x 104 HeLa or HeLa-sgLRRC15 cells (1:4 ratio) were co-plated per well in 8-well chamber slides (Nunc). The following day ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Molecular Biology 2019Quote: Samples were loaded onto 8% SDS-polyacrylamide gels or 4–12% Tris/HEPES-SDS Bolt™ precast gels (Thermo-Invitrogen) for electrophoresis ...
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bioRxiv - Bioengineering 2021Quote: ... the mouse Achilles tendon samples were stained with 0.2% of 4 mg/ml fluorescein diacetate (FDA) (596-09-8, ACROS Organics) and 0.5% of 1 mg/ml propidium iodide (PI ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... and then cells lysates were prepared using RIPA lysis buffer as described previously.30 Total proteins (8 µg/sample) were loaded onto 4%-20% Novex Tris-Glycine gels (Invitrogen), transferred onto polyvinylidene fluoride membrane ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... 200 mM Tris-HCl, pH 8.0, 8 M Urea, 4% CHAPS, 1 M NaCl, supplemented with protease and phosphatase inhibitors, Thermo Scientific) was added to the pellets and the extracts were sonicated 10 x 3s with a probe sonicator ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 15,000×g for 15 minutes at 4°C (ThermoFisher Fiberlite F15s-8×50c rotor). The cell pellets were washed in 100ml of 30mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000g for 30 min at 4°C with a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T was co-transfected with lentiviral expression constructs (12 µg), viral packaging plasmid (psPAX2, 8 µg), and viral envelope plasmid (pMD2.G, 4 µg) using Lipofectamine 2000 (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and plated onto coverslips (1:2 split) or Nunc 8 well chamber slides (Thermo Fisher # 155409PK) (20-40k cells per well).
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
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bioRxiv - Microbiology 2021Quote: ... The library was subsequently cloned into the plasmid pCR™8/GW/TOPO™using the pCR™8/GW/TOPO™ TA Cloning Kit (Thermofisher).
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bioRxiv - Plant Biology 2021Quote: ... The cells were then washed in PBS and placed individually on the cap of an 8-tube PCR strip (Axygen Low Profile 8-Strip PCR Tubes; Fisher Scientific 14-223-505). The presence of a single meiotic cell without attached debris was confirmed microscopically during both pick-up and release (10X magnification ...
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bioRxiv - Developmental Biology 2022Quote: ... was placed into a 6-well tissue culture dish (0.8-1.0ml) and the 6-well plate fitted with Falcon™ Cell Culture Inserts with 8 um pore size (Fisher Scientific, 08-771-20). The lungs were transferred from the holding medium onto the membrane with a sterile razor blade and 5-10 ul of holding media to keep the lungs wet ...
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bioRxiv - Microbiology 2020Quote: ... washed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA, USA) two times ...
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bioRxiv - Microbiology 2021Quote: ... and 10 ml of 1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Life Technologies). Sf9 insect cells (ATCC CRL-1711 ...
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bioRxiv - Bioengineering 2022Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 1 M was purchased from Gibco (Waltham, MA), sodium chloride 5 M was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 M guanidine hydrochloride (GuHCl) was obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... supplied with 8 U/ml protease K (Thermo Fisher, AM2548) and for expansion of Neisseria additional 1 mg/ml Lysozyme according to Lim et al.45 Digested gels were expanded in hourly changed ddH2O until the expansion saturated ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 125 μl in 8-well lab-Tek chambered coverglass (Nunc); or 20 μl in 384-well glass bottom SensoPlate (Greiner) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 8-well chamber slides (Nunc Lab-Tek chamber slide system ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with Essential 8 Flex Supplement (50x, Thermo Fisher Scientific) and penicillin–streptomycin (0.1% ...
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bioRxiv - Physiology 2022Quote: Cells plated on 8-well Chamber Slides (Thermo Scientific, 154534) with indicated treatments were fixed with 4% formaldehyde and permeabilized with 0.2% Triton-X 100 at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Commissural neurons were grown on 8-well LabTek slides (Nunc) coated with poly-L-Lysine (20 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Commissural explants were grown on 8-well LabTek slides (Nunc) coated with poly-Lysine (20 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... TE buffer 1X (10mM Tris, 1mM EDTA, pH 8 - Invitrogen) and 0,7X AMpure beads to procede with sample purification) ...
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bioRxiv - Genomics 2020Quote: ... We used 8 μL anti-rabbit IgG Dynabeads (Life Technologies) for CTCF or Rad21 antibodies and washed the beads with cold RIPA buffer 1 (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were resolved on 8% acrylamide gel (ThermoFisher Scientific), transferred to nitrocellulose membrane ...
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bioRxiv - Neuroscience 2019Quote: ... with 8% FBS (Teak Serum) and 1% Pen-Strep (Gibco) and were transfected at 60% confluency with 10µg of Double UP and either no pCAG-Cre or 1ng pCAG-Cre using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... were maintained in Essential 8 Flex medium (Gibco, A28583-01) supplemented with Essential 8 Flex Supplement (Gibco ...