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Citations for Thermo Fisher :
501 - 550 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 7 μg/ml polybrene Polybrene (Thermo Fisher Scientific GmbH 11360039) was added to each viral sample ...
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bioRxiv - Microbiology 2023Quote: ... in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). The relative change of viral RNA between the compound-treated infection samples and the control infection samples was calculated using the ΔCt values ...
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bioRxiv - Microbiology 2023Quote: ... A ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) was used to amplify and quantify cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... using a ViiA 7 Real-Time PCR system (Thermo Fisher). Ct values from n=6 (three measurements from two independent experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... using a ViiA 7 Real-Time PCR system (Applied Biosystems) along with primers ...
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bioRxiv - Plant Biology 2023Quote: ... according to manufacturer’s instructions on a ViiA 7 system (ThermoFisher). Primers are listed in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... The QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) was used for Real-time PCR reactions with the Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen, # D399), was used to estimate Reactive Oxygen Species (ROS ...
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bioRxiv - Immunology 2023Quote: ... Zeba Spin Desalting Columns (7 K MWCO, 2ml. Thermo Fisher) were then used to separate crosslinked proteins from excess crosslinker and reaction byproducts ...
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bioRxiv - Cell Biology 2023Quote: ... 7-AAD viability staining solution (Thermo Fisher Scientific, 00-6993) was added ...
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bioRxiv - Cell Biology 2023Quote: ... The QuantStudio 7 Flex real-time PCR system (Applied Biosystems) with the following program was employed for qPCR analysis ...
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bioRxiv - Biophysics 2022Quote: ... MCF-7 cells were cultured in MEM (Thermo fisher, 11095072) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... on a QuantStudio 7 Flex Real-Time PCR System (ThermoFisher). Relative gene expression was determined using the change-in-threshold (2-DDCT ...
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bioRxiv - Cancer Biology 2022Quote: ... CellEvent Caspase-3/7 Green Flow Cytometry Kit (Thermo Fisher) was used to measure fraction of apoptotic cells by flow cytometry (BD LSR Fortessa with HTS sampler) ...
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bioRxiv - Developmental Biology 2023Quote: ... 7% glycerol) containing protease and phosphatase inhibitors (Thermo Scientific; 1861281). Cells were scraped into a 1.5-mL tube ...
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bioRxiv - Biophysics 2023Quote: ... 7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen. USA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...
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bioRxiv - Immunology 2023Quote: ... on a QuantStudio 7 Flex Real-Time PCR System (ThermoFisher) in 384-well plates (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Invitrogen CellEvent Caspase-3/7 green detection reagent (C10423, ThermoFisher) was used as stated in manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... on a ViiA 7 Real-Time PCR System (Applied Biosystems), using the following primers ...
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bioRxiv - Cancer Biology 2024Quote: ... CellEvent Caspase-3/7 Green ReadyProbes™ Reagent (Invitrogen, R37111) was used ...
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bioRxiv - Cell Biology 2023Quote: ... on a QuantStudio 7 Flex Real-Time PCR System (ThermoFisher) in 384 well plates (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... on the ViiA 7 Real-Time PCR instrument (Applied Biosystems). The following primers were used for Gli1 (Fwd GAG GTT GGG ATG AAG AAG CA ...
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bioRxiv - Biochemistry 2023Quote: ... Chromatograms were analyzed using Chromeleon 7 software (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Data were processed using ViiA™ 7 Software (Applied Biosystems) and analyzed with the 2(−ΔCT ...
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bioRxiv - Physiology 2023Quote: ... with the ViiA 7 Real-Time PCR System (Applied Biosystems). Relative mRNA levels were normalized to GAPDH ...
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bioRxiv - Plant Biology 2024Quote: ... in a ViiA 7 Real-time PCR system (Applied Biosystems) for 40 cycles with two steps per cycle ...
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bioRxiv - Cell Biology 2023Quote: ... and analyzed on the QuantStudio 7 system (Thermo Fisher Scientific) using the standard sybergreen protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and COS-7 cells using Lipofectamine 2000 (Thermo Fisher Scientific), Lipofectamine LTX (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... on the QuantStudio 7 Real-Time PCR System (ThermoFisher Scientific). mRNA expression levels for Osmr (Taqman probe #Mm01307326_m1 ...
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bioRxiv - Genomics 2023Quote: ... on a ViiA-7 Real-Time PCR thermocycler (Applied Biosystems). RNA levels were normalized against Beta-actin reference gene following the 2-ΔCt method ...
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bioRxiv - Molecular Biology 2024Quote: ... a Caspase-3/7 assay kit was used (Invitrogen, C10427). Each tube was brought to a volume of 1 ml staining buffer and 1 μl of CellEvent Caspase-3/7 reagent was added ...
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bioRxiv - Molecular Biology 2024Quote: ... in a ViiA 7 Real-Time PCR machine (Life Technologies). When measuring ‘A-tailed’ RNA levels ...
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bioRxiv - Microbiology 2024Quote: ... and QuantStudio 7 Flex Real-Time PCR Systems (Applied Biosystems). Transcript copy numbers of target genes were determined using the QuantStudio Real-Time PCR Software v1.3 and then normalized to the housekeeping transcript for Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... Hela and MCF-7 cells using TRIzoL (Thermo Fisher Scientific). RNA was reversely transcribed to cDNA via iScriptTM Reverse Transcription Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed on a QuantStudio 7 Pro (Applied Biosystems) and analyzed using Design and Analysis v2.6 software (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 7 µm sections were cut with a cryostat (Thermo Scientific, blade temperature ...
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bioRxiv - Immunology 2024Quote: ... and a ViiA 7 Real-Time PCR System (ThermoFisher Scientific) as described (Andersson et al. ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were immersed in a 1:1-mixture of ethanol and methyl salicylate (Fisher Scientific GmbH, Schwerte, Germany) for 20 min and then in 100% methyl salicylate for about 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... freshly mitochondria isolated from pupae were resuspended in respiration buffer (0.5 mg/ml) containing Tetramethylrhodamine methyl ester perchlorate (0.5 μM) (Thermo Fisher). The excitation spectra were scanned from 520 nm to 580 nm using 590 nm emission wavelengths ...
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bioRxiv - Microbiology 2021Quote: ... and cells were overlayed with carboxy-methyl cellulose (CMC) containing media (0.6% CMC, MEM supplemented with 1X Penicillin/Streptomycin (Gibco), 2% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:25∼28 molar ratio for 2 hrs on ice ...
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bioRxiv - Neuroscience 2022Quote: [Ca2+]c was measured by ratiometric analysis using acetoxy-methyl-ester Fura-2 (Fura-2/AM; F1221 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 500 µL of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) (Thermo Scientific, TS48915) and heated for 1 h at 70 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... To count the apoptotic events cells were treated with a fluorescent dye detecting Casp3/7 activation (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
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bioRxiv - Neuroscience 2019Quote: ... were designed based on the appropriate National Center for Biotechnology Information (NCBI) reference genome using the Methyl Primer Express v1.0 software (Thermofisher Scientific) to amplify 250-350 bp regions of interest upstream or downstream the transcription start site (TSS ...