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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: 0.5 M EDTA pH 8.0 (Invitrogen™/Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Epoxy M-270 beads (14301, Invitrogen) were coated with 40 μg of either aDB1-prep3-730 or aDB1-prep3-730-P peptides ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 M NaCl (AM9760G, Ambion) freshly supplemented with 10 U/ ml proteinase K (P8107S ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 M (#AM9260G, Thermo Fisher Scientific); 1 mM phenylmethanesulfonyl fluoride (#93482 ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Neuroscience 2019Quote: ... were loaded at 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 98% buffer A ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated upon shaking at the growth temperature for 5 min with following concentrations: FM 5-95 (2 µg/ml; Thermo Fisher Scientific), DiSC3(5 ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA sequences targeting CD33 exon 2 were 5’-TCCATAGCCAGGGCCCCTGT and 5’-GCATGTGACAGGTGAGGCAC.25 U937 cells were washed three times in PBS (Gibco 10010-023) and resuspended in complete Nucleofector Kit C (Lonza Biosciences VCA-1004 ...
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bioRxiv - Pathology 2020Quote: ... Aliquots of 15 μL per sample were loaded at a rate of 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) which was equilibrated with 98% Buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Developmental Biology 2021Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Developmental Biology 2020Quote: ... HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154, Fisher Scientific, Waltham, MA, USA). Following purification of total RNA ...
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bioRxiv - Cell Biology 2020Quote: ... SKOV3-Red and SKOV3-M (1 × 106 of each) were resuspended in PBS with 5 μg/mL Hoechst 33342 (Life Technologies, Carlsbad, USA), and incubated for 20 min at 37°C in water-bath ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed with PBS and incubated with 100 μL of 5 M H2-DCFDA in PBS (Molecular Probes, catalogue number D399). H2O2 levels were assayed on a fusion spectrofluorimeter (PackardBell ...
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bioRxiv - Neuroscience 2022Quote: Wild-type mice received a single iontophoretic injection of each anterograde tracer: biotinylated dextran amine (BDA; 10 kDa; 5% solution in 0.125 M phosphate buffer, pH 7.4; Thermo Fisher Scientific, Cat. No. D1956) or Phaseolus vulgaris leucoagglutinin (PHA-L ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant containing soluble CCTX2 was then filtered through a 0.22 μm PES membrane filter and loaded onto a 5 mL Tricorn column packed with immobilized D-galactose gel (Pierce™, Thermo Scientific™) previously equilibrated with 6 CV loading buffer (20 mM Na-HEPES ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μl forward primers (5 μM) and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were homogenised with a FastPrep-24 5G instrument using TallPrep Lysing Matrix M tubes (MP, Cat# 116949025) in 2 ml Trizol and RNA was isolated using the RiboPure Kit (Invitrogen, Cat# AM1924), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... Microscopy images for tdTomato and zsGreen fluorescence were taken of the 10-μ;m sections using an EVOS FL Auto 2 Imaging System (Invitrogen, Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2020Quote: ... the dimerized GFP nanobody LaG-16–G4S–LaG-2 (green lobster) was covalently linked to magnetic beads (Dynabeads M-270 epoxy Thermo Fisher Scientific) as previously described (45) ...
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bioRxiv - Microbiology 2020Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The ubiquitylated worm CMG was then incubated for 30 minutes at 4°C with 2 µl (per 10 µl ubiquitylation reaction mix) magnetic beads (Dynabeads M-270 Epoxy; Life Technologies, 14302D) that had been coupled to anti-worm SLD-5 antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA from DNAse-treated samples were reverse transcribed using M-MLV for complementary DNA generation (cDNA) (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s protocol including the options to add 20 μl of 2 M dithiothreitol (Life Technologies/Thermo Scientific; https://www.thermofisher.com) per 1 ml buffer RLC and on-column Dnase digestion with Dnase I (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... and washed for 3min on a C18 reverse phase precolumn (3μm particle size, 100 Å pore size, 75 m inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6μm particle size ...
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bioRxiv - Cell Biology 2023Quote: ... connected to an analytical column (EASY-Spray column, 50 m × 75 μm ID, PepMap RSLC C18, 2 μm, 100 Å, Thermo Scientific) using the autosampler of an Easy nLC 1000 (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... we used 0.2 μm diameter carboxylate-modified polystyrene fluorescent microspheres (FluoSpheres, far red/FR λ=660/680, 2% solid diluted in water, F8807, ThermoFisher Scientific) that were activated to promote covalent coupling with primary amines of proteins exposed on the parasite surface ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA (2-5 µg) was treated with DNAse I (Thermo Fisher Scientific, Cat # AM1906) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then blocked with 2% BSA and 5% donkey serum (ThermoFisher Cat # NC9624464) in PBS for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
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bioRxiv - Genomics 2020Quote: ... 5 µl of 10X Turbo DNase buffer and 2 µl Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then reduced with 5 mM Tris(2-carboxyethyl)phosphine (ThermoFisher Scientific) for 30 minutes at 37 °C and then alkylated in the dark for 30 minutes at 25 °C with 40 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: ... Grids were blotted for 2–5 s in a Vitrobot (Mark IV, Thermo Fisher) at 10–15 °C and 100% humidity ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
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bioRxiv - Neuroscience 2021Quote: ... and two injections of 5-ethynyl-2’-deoxyuridine (EdU: 0.01 mg/kg, Invitrogen #A10044), made as a 10 mM stock solution in DMSO and diluted for injection to 40% strength in 0.9% sterile saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... and were supplemented with 10mM EdU (5-ethyl-2’-deoxyuridine) (ThermoFisher Scientific, Rockford, IL) during the final 6 hours of incubation to mark proliferating cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mixed with 5 μl of 2 x Novex TBE-Urea sample buffer (ThermoFisher Scientific) and denatured for 3 minutes at 70°C ...
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bioRxiv - Molecular Biology 2022Quote: ... For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg ...
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bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...