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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004, Invitrogen, 1:5000) 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... After incubation for 1h with the appropriate secondary antibodies (Alexa Fluor 488 or 594 (Thermo Fisher)) ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 1h on ice with or without RNase (10μg/mL RNase A) (Thermo Fisher). Lysate was centrifuged at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: Cells were harvested via centrifugation at 21,000xg 4°C 1h (Thermo scientific, Sorvall Lynx 4000 centrifuge), the supernatant discarded and the resulting pellet resuspended in 1ml of spent medium ...
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bioRxiv - Cell Biology 2022Quote: ... followed by PBS washes (3x) and staining with Hoechst (Life Technologies, 1h, 1:10000 in PBS). Cells were imaged using a 20x water immersion objective on a high content confocal microscope (Molecular Devices™ ImageXpress Micro XL).
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bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1h followed by incubation with goat α-rabbit Alexa Flour 488 (Thermo Fisher, A-11008) for 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and goat anti-mouse Alexa Fluor 488 secondary antibody (1:500, 1h; A-21121, Invitrogen, Germany) were used ...
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bioRxiv - Genomics 2022Quote: ... primary incubation was performed for 1h (anti-fibrillarin monoclonal mouse, 1:100, MA3-16771, Thermo Scientific), followed by incubation with secondary antibody (anti-mouse monoclonal FITC ...
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bioRxiv - Plant Biology 2022Quote: ... with and without the addition of 3-Amino-1H-1,2,4-triazole (Acros Organics (Thermo Fisher Scientific)) to test the strength of the interaction.
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bioRxiv - Plant Biology 2022Quote: ... with and without the addition of 3-Amino-1H-1,2,4-triazole (Acros Organics (Thermo Fisher Scientific)) to test the strength of the interaction.
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bioRxiv - Developmental Biology 2023Quote: ... after 1h of secondary antibody incubation fluorochrome conjugated phalloidin (Alexa Fluor™ 546 Phalloidin, Invitrogen™) was added to the secondary antibody solution for F-actin membrane staining for another hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Each coverslip was then incubated for 1h at 37ºC with Duolink PLA probes (Thermo Fisher Scientific) diluted in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Cut samples were incubated 1h at 37°C with Acridine orange 10 μM (A1301, Thermo Fisher), with or without Histamine 100 μM (H7127 ...
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bioRxiv - Genetics 2024Quote: ... followed by 1h incubation in the dark with anti-mouse Alexa Fluor 350 (1:50) (Invitrogen). DNA fibers were then visualized and imaged as described above (DNA fiber analysis).
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Developmental Biology 2022Quote: ... injected fluorescent oocytes were selected and matured with 1-methyl adenine (3.0 μM Acros Organics). Eggs were fertilized with diluted sperm ...
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bioRxiv - Plant Biology 2021Quote: ... 30 μL of N-methyl-N-trimethylsilyltrifluoroacetamide plus 1% trimethylchlorosilane (MSTFA + 1% TMCS, Thermo Scientific) was added to the MSTFA vials ...
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bioRxiv - Biophysics 2021Quote: ... A Methyl-β-Cyclodextrin solution (200 mM) in Live Cell Imaging Solution (Thermo Fisher Scientific) was added to the cholesterol lipid film at a molar ratio of 10 to 1 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then pegylated with MS(PEG)8 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) for 15 minutes prior to grid preparation.
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bioRxiv - Physiology 2024Quote: ... for 1 hour and then incubated with Calcium Orange AM methyl ester (Invitrogen Cat. # C3015) + 0.01% Pluronic acid (Invitrogen Cat ...
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bioRxiv - Microbiology 2024Quote: ... Methyl-esterified free fatty acids were analyzed using a ThermoFisher Exactive GC mass spectrometer (ThermoFisher), and ions were introduced by electron impact ionization (70 eV) ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated upon shaking at the growth temperature for 5 min with following concentrations: FM 5-95 (2 µg/ml; Thermo Fisher Scientific), DiSC3(5 ...
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bioRxiv - Cell Biology 2021Quote: ... The sgRNA sequences targeting CD33 exon 2 were 5’-TCCATAGCCAGGGCCCCTGT and 5’-GCATGTGACAGGTGAGGCAC.25 U937 cells were washed three times in PBS (Gibco 10010-023) and resuspended in complete Nucleofector Kit C (Lonza Biosciences VCA-1004 ...
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bioRxiv - Pathology 2020Quote: ... Aliquots of 15 μL per sample were loaded at a rate of 5 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) which was equilibrated with 98% Buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at 37°C (with 5% CO2 and humidity) before indicator loading with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA (2-5 µg) was treated with DNAse I (Thermo Fisher Scientific, Cat # AM1906) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then blocked with 2% BSA and 5% donkey serum (ThermoFisher Cat # NC9624464) in PBS for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
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bioRxiv - Genomics 2020Quote: ... 5 µl of 10X Turbo DNase buffer and 2 µl Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then reduced with 5 mM Tris(2-carboxyethyl)phosphine (ThermoFisher Scientific) for 30 minutes at 37 °C and then alkylated in the dark for 30 minutes at 25 °C with 40 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
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bioRxiv - Neuroscience 2021Quote: ... and two injections of 5-ethynyl-2’-deoxyuridine (EdU: 0.01 mg/kg, Invitrogen #A10044), made as a 10 mM stock solution in DMSO and diluted for injection to 40% strength in 0.9% sterile saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... and were supplemented with 10mM EdU (5-ethyl-2’-deoxyuridine) (ThermoFisher Scientific, Rockford, IL) during the final 6 hours of incubation to mark proliferating cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mixed with 5 μl of 2 x Novex TBE-Urea sample buffer (ThermoFisher Scientific) and denatured for 3 minutes at 70°C ...
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bioRxiv - Molecular Biology 2022Quote: ... For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg ...
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bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...