Labshake search
Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for Recombinant Rabbit IFNB1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Recombinant MARV-Angola GPdTM (IBT Bioservices) was tethered to Fluospheres NutrAvidin-Microspheres yellow-green or red (Thermo Fisher Scientific, Waltham, MA) using the EZ-link Micro Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
-
bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
-
bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
-
bioRxiv - Molecular Biology 2022Quote: ... using conditions recommended by the supplier (1× PNK buffer without ATP, 2 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 2 h) ...
-
bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...
-
bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 700µl FACS buffer with 3.5ul recombinant RNase inhibitor and Hoechst dye (Thermo Fisher, H3570, 1:2000) and incubated 4min on ice covered from light ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
-
bioRxiv - Genomics 2022Quote: ... 2.5uL of the Slic Reaction is used for subcloning to generate a recombinant donor pFast vector with the Bac-to-Bac™ Baculovirus Expression System (Gibco) as per manufacturer instructions using TOP10 E ...
-
bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg HexaPro) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
-
bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
-
bioRxiv - Microbiology 2023Quote: ... and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2023Quote: 20µg of rhEPO and reference product Eprex® were treated with 20 units of recombinant PNGase F (Thermo Fisher Scientific, USA) at 37 ºC for hours ...
-
bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
-
bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
-
bioRxiv - Cancer Biology 2023Quote: ... LN single cell suspension were stimulated for 5h at 37°C with 5ug/ml of LCMV-GP61-80 in the presence of 50 U/ml recombinant murine IL2 (Thermofisher P2747) and 1mg/ml Brefeldin A (Sigma) ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
-
bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
-
bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
-
Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
-
bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
-
bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were detected with goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 rabbit (Thermo Fisher Scientific Cat# A32731TR ...
-
bioRxiv - Neuroscience 2020Quote: ... the primary antibody rabbit anti-GFP (Sysy 132002, RRID:AB_887725) and the secondary antibody AlexaFluor 488 goat anti-rabbit (Invitrogen).
-
bioRxiv - Neuroscience 2020Quote: ... and a secondary anti-rabbit antibody (donkey anti-rabbit Alexa Fluor 488 (1:1000, #A21206, Thermo Fisher Scientific). Images were acquired with a fluorescent microscope (BZX800 ...
-
bioRxiv - Molecular Biology 2022Quote: ... rabbit monoclonal anti-LIMP2 (SCARB2; #702770) and rabbit polyclonal anti-FASN (#PA5-22061) antibodies were purchased from ThermoFisher. Mouse monoclonal anti-IRS4 (#sc-100854 ...
-
bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 568 anti-rabbit IgG (A11036) and Alexa Fluor 647 anti-rabbit IgG (A21245) from Thermo Fisher.
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
-
bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit COL5A1 (1:500; #PA5-83158) and anti-rabbit SIX3 (1:200; #PA5-103901) from Thermo Fisher; anti-mouse COL3A1 (1:300 ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary goat anti-rabbit Alexa Fluor 546 or goat anti-rabbit Alexa Fluor 488 antibodies (1:1000; Invitrogen) were incubated for one hour ...
-
bioRxiv - Neuroscience 2022Quote: ... and a secondary anti-rabbit antibody (donkey anti-rabbit Alexa Fluor 488 (1:1000, #A21206, Thermo Fisher Scientific). Images were acquired with a fluorescent microscope (BZX800 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rabbit anti-SDHC or rabbit anti-SDHD antibody (1:200, AB_2634976 and 1:200, AB_2717499, Fisher Scientific, Germany) as well as mouse anti-CD4 monoclonal antibody 4B12 (1:1000 ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were incubated with an anti-rabbit secondary antibody (1:300 AlexaFluor 594nm goat anti-rabbit, Invitrogen A11037), nuclei were stained with Hoechst 33342 (Invitrogen™ R37605 ...
-
bioRxiv - Cell Biology 2023Quote: ... Alexa-594 donkey anti-rabbit/anti-mouse or Alexa-700 donkey anti-rabbit secondary antibodies (ThermoFisher Scientific, UK) and DAPI (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... and rabbit anti-lysozyme (1:500, Novus) with goat anti-rabbit IgG Alexa Fluor 568 (Thermo Fisher Scientific); rabbit anti-villin (1:1000 ...
-
bioRxiv - Biochemistry 2024Quote: ... Alexa 488-conjugated anti-rabbit IgG and Alexa 633-conjugated anti-rabbit IgG (all from Thermo Fisher Scientific). Mitochondria were stained with MitoTracker Orange CMXRos (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were incubated with an anti-rabbit secondary antibody (1:300 AlexaFluor 594nm goat anti-rabbit, Invitrogen A11037), nuclei were stained with Hoechst 33342 (Invitrogen™ R37605 ...