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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... samples were incubated in the presence of an anti CD16/32 Fc receptor-blocking antibody followed by surface staining of the following antibodies in three panels: Panel 1: APC-Cy7 CD3, FITC CD4, PerCP-Cy5.5 CD8, APC CD25 (BD 557596, 551162, 565159) (ThermoFisher 11-0042-86, 25-0441-82, CD25); Panel 2 ...
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bioRxiv - Microbiology 2024Quote: ... Briefly, the Accudenz collected cells were added to in vitro ookinete media (RPMI 1640, 20% v/v FBS (Fisher Scientific, Cat#350-11-CV Lot # 35011126), 0.05% w/v hypoxanthine (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... A cutoff on the endothelial cells in the channel of the adhesion molecule antibody (E-selectin [Santa Cruz Biotechnology: sc-59766 PE] and VCAM1 [Life Technologies: 11-1061-82 FITC]) was used to identify high expressing cells within the BMECs ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification was performed using a range of 1:1 to 1:11 ratio of β-tubulin and plasmid containing the gene of interest to calculate a standard curve (Thermo Fisher Design and Analysis software). Expression analysis calculation was performed using the ΔΔCq method ...
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bioRxiv - Pathology 2021Quote: RNA samples containing residual DNA contaminants were further treated with 2 μL of buffer (10×) and 2 μL of TurboTM DNAse (2 U/μl) (Ambion®). Samples were incubated at 37°C for 30 min followed by DNAse inactivation by adding 2 μL of DNAse Inactivation Reagent (Ambion®) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked in 2% goat serum + 2% Bovine Serum Albumin for 2 hours at room temperature before primary antibody (GFP, 1:500, Invitrogen #A6455) was added and the embryos were incubated overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% heat-inactivated autologous plasma and 2 mM L-glutamine (Life Technologies), unless otherwise stated ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Neuroscience 2022Quote: ... GlutaMAX and FGF-2 (2 ng/ml) (all reagents from Thermo Fisher Scientific) in 24-well plates for 2 weeks on coverslips coated with poly-D-lysine (100 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... 2 min) to nitrocellulose membranes using an iBlot 2 Dry Blotting System (Invitrogen). Membranes were briefly washed in PBS-T (0.1% Tween-20/PBS) ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 2 mA cm−2 in a semi-dry electroblotter (Thermo Fisher Scientific) for 45 min using 1 × TBE buffer ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 U/ml of interleukin-2 (IL-2; Thermo Fisher Scientific, PHC0026) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
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bioRxiv - Immunology 2021Quote: ... EBM-2/EGM-2 medium was supplemented with puromycin (1 mg/ml, Gibco) for 3 d ...
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bioRxiv - Immunology 2022Quote: ... 2 h goat anti-rabbit IgG F(ab’)2-AF488 secondary antibody (Invitrogen), 15 min DAPI (1:1000) ...
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bioRxiv - Neuroscience 2024Quote: ... 150uL of Tyrode’s containing 2 uM Fura 2-AM (Invitrogen F1221, Lot 2559176)/ Pluronic F-127 (Invitrogen P3000MP ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µL Invitrogen TURBO DNase and 2 µL SUPERase•In RNase Inhibitor (Invitrogen). After digest ...
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bioRxiv - Microbiology 2023Quote: ... for adipose fat or 2 mg/mL collagenase type 2 (ThermoFisher Scientific, 17101015) for lung samples ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Myocytes were then loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously (Batiste et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 2 μM Fura-2 AM (Thermo Fisher Scientific, F1225) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mM glutamine and 2 mM HEPES (Thermo Fisher Scientific, Waltham, MA, USA). Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco 15630080) and 1 % penicillin-streptomycin (PS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10% glycerol (2 mM 2-mercaptoethanol (BME) and protease inhibitor tablet (ThermoFisher Scientific) added immediately before use) ...
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bioRxiv - Neuroscience 2019Quote: Plated DRG cultures were loaded with fura-2 by 1-hour incubation with a mixture of 2 μM fura-2 AM (Thermo Fisher Scientific) and 0.01% pluronic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Cell Biology 2020Quote: S phase status was monitored by measuring 5-ethynyl-2’-deoxyuridine (EdU) incorporation with the Click-iT™ Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (ThermoFisher, C10632) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg of RNA were reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit with RNAse Inhibitor (Applied Biosystems, Vilnius, Lithuania). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Lungs were homogenised with a FastPrep-24 5G instrument using TallPrep Lysing Matrix M tubes (MP, Cat# 116949025) in 2 ml Trizol and RNA was isolated using the RiboPure Kit (Invitrogen, Cat# AM1924), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were pulse labeled for 2 hours with 10 µM EdU and stained using the Click-iT EdU Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... To create the lag-2 full-length construct we used the Multisite-Gateway Three-Fragment Vector construction kit (Thermo Fisher Scientific, 2012). 60ng /µl of this 17.623bp long lag-2 full-length construct was injected together with the co-injection markers (pCFJ601 50ng/µl ...
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bioRxiv - Cell Biology 2019Quote: ... staining was performed in three-dimensional cysts with 2 mM calcein AM and 4 mM ethidium homodimer I (Live/Dead® Viability/Cytotoxicity kit; Life Technologies) for 40 min at 37°C in the dark ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were visualized in a 2% agarose stained by ethidium bromide to check for RNA integrity and quantify using Qubit™ RNA BR Assay Kit (ThermoFisher, Q10210). gRNAs targeting tbxta and tyr CDS in zebrafish and rx3 in medaka were in vitro transcribed from PCR containing a pool of different gRNA primers at equal concentration ...
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bioRxiv - Biochemistry 2020Quote: ... Each sample was labeled for 2 h at 22°C with a different TMT-tag obtained from a TMT10plex kit (Thermo Fisher Scientific), and then quenched (0.27% hydroxylamine ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA). Quantitative PCR reactions and measurements were performed with a CFX384 Real-Time PCR detection system or an iQ5 Multi-color real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was treated with 2 U of DNase per 10 μg of total RNA using the TURBO DNA-free™ Kit (Thermo Fisher Scientific, former Ambion). Integrity of DNA-free RNA was verified in Bleach Gel (Aranda et al. ...
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bioRxiv - Systems Biology 2021Quote: Cells were harvested at a concentration of 1-3 × 106 cells/mL and resuspended at 2 × 107 cells/mL in 800 μL of Reagent A from the Mitochondrial Isolation Kit for Cultured Cells (Thermo Fisher #89874) on ice ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 µg of total RNA using oligo(dT) primers and Superscript II First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Quantitative RT-PCR was performed with FastStart Universal SYBR Green Master Mix and a LightCycler 96 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified RNA (2 µg) was used to perform cDNA synthesis using the ABI High Capacity cDNA Reverse Transcription Kit (Thermo Fisher 4368814) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from Caco-2 cells grown in 24 well plates for 21 days using the PureLink RNA Mini kit (Thermo Fisher, 12183025) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... (all the primer sequences are listed in Table 1 and the PCR conditions are listed in Table 2) respectively.The PCR product were purified using Quick gel extraction kit (Invitrogen: Cat no. # K210012). The purified double-stranded 5′UTR fragment and 3′UTR fragment (containing overhangs for insert and vector fragment ...